Viruses HeLa cell lines

Chadeuf, G. et al. (2000). Efficient recombinant adeno-associated virus production by a stable rep-cap HeLa cell line correlates with adenovirus-induced amplification of the integrated rep-cap genome. J. Gene Med. 2, 260-268. 

We recently reported the synthesis of polymeric platinum II derivatives of cis-dichlorodiamineplatinum II, cis-DDP (2 1,2). These polyamines (2) exhibit good inhibition towards a wide range of tumor cells including L929, WISH and HeLa cell lines with good cell differentiation. Some extend the life of (induced) terminally ill cancerous mice by 50%. Further the polyamines exhibit activity towards virus at concentration levels where the cells are unaffected. Recent biological studies are aimed at evaluation of certain polyamines on inhibiting select tumors and viruses for specific disease control. 

Polio virus Ptualysis, Aseptic meningitis RNA polymerase assay Plaque assay, RT-PCR (Hela cell line)... 

There are now available a number of lines of cells, mainly originating from malignant tissue, which can be serially subcultured apparently indefinitely. These established cell lines are particularly convenient as they eliminate the requirement for fresh animal tissue for such sets or series of cultures. An example of these continuous cell lines are the famous HeLa cells, which were originally isolated from a cervical carcinoma of a woman called Henrietta Lacks, long since dead but whose cells have been used in laboratories all over the world to grow viruses. 

This is a widely studied cell line derived from a human cervix adenocarcinoma (Gey et al, 1952). The cells are epithelial-like in morphology and are susceptible to polio virus type 1 and adenovirus type 3. HeLa cells are used for the expression of recombinant proteins, including mouse metallothionein 1 gene, human Cu/Zn superoxide dismutase and hepatitis B surface antigen. They have been widely used as an in vitro model system because of the ease with which they can be cultivated but one drawback of this is that the cell line has been responsible for widespread contamination of other cell lines (Nelson-Rees et al, 1981). 

Cell-free virus or infected cells (infectious centers, ICs) can be measured at frequencies as low as 10 6. The sensitivity of the FIA rivals that of PCR, where examining the input from 106 cells is an upper limit. The FIA detects all strains of HIV, including primary isolates from patients (9,57,61,64,67-69). ICs may be detected in tissue culture cell lines and PHA blast cultures infected in vitro. The assay has also been used to quantify infectious HIV in AIDS patients (69). The assay is performed by plating either infected cells or cell-free virus on a monolayer of HeLa cells expressing CD4, CXCR4, and CCR5. Detection of clinical isolates is highly dependent on the HeLa-CD4 cell line used and is a... 

Studies of the role of viruses in chromosomal anomalies are of two types the studies in which the infection takes place in vivo and the infected cells, usually leukocytes, are cultured in vitro to study chromosomal anomalies [184-187] and studies in which the effect of viruses on the mammalian karyotype has been investigated in vitro using either primary cell cultures or cell lines (cells capable of indefinite division—in humans, at least 70 consecutive divisions with 3-day intervals between cell subculture). When primary cell cultures are used (cells freshly derived from a living organism), usually lymphocytes stimulated with phytohemagglutinin are infected with the virus. Since only one cell division can be observed in primary cell cultures, established cell lines, such as HeLa cells, would seem to be preferable for studying the effect of viruses on the karyotype. But these cultures are heteropioid, and therefore most cell lines are inadequate for chromosomal studies. Fortunately, diploid cell lines are now available. 

---