Unscheduled DNA synthesis assay

Hoechst. 1984d. Evaluation of Hoe 002671 - substance technical - in the rat primary hepatocyte unscheduled DNA synthesis assay Final report. Conducted for Hoechst Aktiengesellschaft, Frankfurt, Germany. Litton Bionetics, Inc., Kensington, MA. FBI project no. 10400-001.[unpublished study]... 

Chlordecone (1.67-10.00 mg/L) increased the frequency of sister chromatid exchange in CHO cells but only in the absence of S9 activation and only in the presence of cell-cycle delay the results were confirmed in a repeat trial (Galloway et al. 1987). By contrast, chlordecone alcohol was negative for sister chromatid exchange induction both with and without S9 activation (Galloway et al. 1987). Subcytotoxic doses of mirex did not induce unscheduled DNA synthesis in primary hepatocytes recovered from rats, mice, or hamsters (Maslansky and Williams 1981 Williams 1980). Similar results were obtained by Probst et al. (1981) using primary rat hepatocytes exposed to 1,000 pmol/L mirex. Chlordecone was also uniformly negative in unscheduled DNA synthesis assays of primary rat hepatocytes (Probst et al. 1981 Williams 1980). 

There were no indications of genotoxic potential for 2,4-D acid, or any of its derivatives, in bacterial assays, in unscheduled DNA synthesis assay, or in mouse bone marrow micronucleus tests. ... 

Charles JM, Cunny HC, Wilson RD, et al Ames assays and unscheduled DNA synthesis assays on 2,4-dichlorophenoxyacetic acid and its derivatives. Mutat Res 444 207-16, 1999... 

Nitropropane is genotoxic in a variety of assays including the kmes Salmonella assay, in vitro sister chromatid exchange, and chromosome aberrations and unscheduled DNA synthesis assay. ... 

Metabolism and genetic toxicity have been reported to differ with the isomer of nitro-toluene. p-Nitrotoluene was not mutagenic in bacterial assays, but it did increase sister chromatid exchange frequencies and chromosomal aberrations in vitro-, in vivo it did not increase the frequency of micronuclei in bone marrow of treated rodents. Similar findings were reported for the ortho isomer, except that it did not induce chromosomal aberrations in vitro. Only the ortho isomer induces DNA excision repair in the in vivo-in vitro hepatocyte unscheduled DNA synthesis assay. Furthermore, ort/jo-nitrotoluene binds to hepatic DNA to a much greater extent than meta- or para-nitrotoluene, and investigators suggest that it may act similarly to the rodent hepatocarcino-gen 2,6-dinitrotoluene. ... 

Hazleton Labs. 1988e. Mutagenicity tests on meta-cresol in a rat primaryhepatocyte unscheduled DNA synthesis assay. Unpublished data submitted to EPA/OTS. Fiche no. OTS0517692. 

Litton Bionetics (1982) Evaluation of Triethanolamine No. 80/175 in the Primary Rat Hepatocyte Unscheduled DNA Synthesis Assay [cited in Knaak et al, 1997]... 

Ethylene dibromide caused a dose-dependent increase in liver DNA alkaline-labile sites and single-strand breaks (as determined by alkaline elution assay) in female Sprague-Dawley rats. It was positive in an unscheduled DNA synthesis assay in rat spermatocytes in vitro but was negative in the spermatocytes of rats dosed in vivo. Ethylene dibromide gave positive results in an amphibian Pleurodeles yvaltl) mieronueleus test but gave negative results in dominant lethal tests. 

Hodgson JR, Myhr BC, McKeon M, et al. 1982. Evaluation of di-(2-ethylhexyl)phthalate and its major metabolites in the primary rat hepatocyte unscheduled DNA synthesis assay. Environ Mutagen 4388. 

Mirsalis JC, Hamilton CM, O Loughlin KG, et al. 1996. Chromium(VI) at plausible drinking water concentrations is not genotoxic in the in vivo bone marrow micronucleus or liver unscheduled DNA synthesis assays. Environ Mol Mutagen 28 60-63. 

Negative results were obtained in Ames and sister chromatid exchange, mouse lymphoma, and unscheduled DNA synthesis assays. 

MEK was not mutagenic in Ames Salmonella) and Escherichia coli tests, but induced aneuploidy in Saccharomyces cerevisiae. MEK was not found to be genotoxic in the mouse lymphoma assay, in Chinese hamster ovary cell, unscheduled DNA synthesis assay, and micronucleus assays. 

Tests for genetic toxicity (Ames assay using Salmonella typhimurium, mouse bone marrow micronucleus assay, chromosome aberrations in human lymphocytes and/or Chinese hamster ovary cells, in vitro unscheduled DNA synthesis assay in primary rat hepatocytes) were all negative. 

Burlinson B, Ashby J. 1988. Inactivity of benzo(a)pyrene in a weanling rat liver, unscheduled DNA synthesis assay. In Evaluation of short-Term tests for carcinogens Report of the International Collaborative Program. Prog Mutat Res 1 389-390. 

Anderson, D., Blowers, S.D., Marrs, T.C., and Rice, P., An in vitro and an in vivo unscheduled DNA synthesis assay with zinc oxide-hexachloroethane (Zn/HCE) smoke. Hum. Exp. Toxicol., 15, 38-44, 1996. 

DNA Adducts Unscheduled DNA Synthesis Assay in Liver Cells Sister-Chromatid Exchange Assay Gene Mutation Assays... 

Amphlett, N. W., Mitchell, 1. D., Rees, R. W., and Haynes, G. A. (1996). A prc xrsal for a two-dose/ single-sample in vivoHn vitro rat hepatocyte unscheduled DNA synthesis assay. Mutagenesis 11, 19-26. 

In the Ames mutagenicity assay, an extract of bloodroot tested weakly positive in Salmonella typhimurium (Ames assay) with metabolic activation, while no mutagenic activity of the compound sanguinarine was observed with or without metabolic activation. No mutagenic activity was observed in E. colt in the unscheduled DNA synthesis assay in rat hepatocytes (Frankos et al. 1990). 

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