Tryptone agar

Rgure 2. Patterns formed by Salmonella [16]. (a) Pattern formation on minimal medium (mannitol plates supplemented with MOPS buffer, pH 7). (b) Pattern of spots formed on tryptone agar (0.3%) photographed 10.5h after inoculation in the center of the plate. Provided by M. Eisenbach and reproduced with the permission of The American Society For Microbiology. 

The G-46 (mutant) was maintained on tryptone agar slants. Swiss... 

Stock culture maintained on Liver tryptone agar stab Liver tryptone agar stab Malt agar slope Liver tryptone agar stab Yeast agar stab i... 

An incubated culture of Actinomyces antibioticus was prepared using a medium consisting of 1% tryptone-peptone, 0.5% starch, 0.2% K HPO, 0.2% NaCI and 0.25% agar in distilled water, grown at a temperature of approximately 25° to 35°C, the incubation being complete after 6 to 10 days. 50 liters of this incubated culture are extracted approximately six times with ether, using 20 liters of ether for each extraction. 

Test Procedure. The test organisms were subcultured onto Tryptone Soya Agar (TSA) slopes and incubated at 37 °C for 24 hours. After incubation for each inoculum, 6 ml sterile distilled water (SDW) was added to each slope in turn, the organisms washed off and the resultant suspension homogenised. A 1 ml aliquot of the suspension was added to 100 mis SDW and homogenised to form the inoculum. 

Materials for media (Bacto Tryptone, Bacto Yeast Extract and Difco SOB Medium, agar powder and NaCl) and antibiotics (ampicillin sodium and kanamycin sulfate). 

Total aerobic viable count pour 1 ml into plate containing 25 ml of tryptone glucose extract agar or TSA half concentration. Incubate at 22°C for 5 days. 

Swab transportation tryptone saline, peptone water, enriched buffered gelatine, buffered saline, buffered gelatine, and brain-heart infusion are generally recommended media that may be used for swab transportation. For RCS + testing agar strip TC (BIOTEST) or M air T air sampler for level I is used. 

Inoculate the surface of tryptone soya agar slant for bacteria and Sabouraud dextrose agar slant for fungi from recently revived stock culture of each of the test microorganisms. 

Promptly pour into each petri dish about 15 to 20 ml of sterile melted tryptone soya agar medium previously melted and cooled to approximately 45°C. 

Note TSA = tryptone soya agar SDA = Sabouraud dextrose agar. 

Rogosa-type medium (60) made as follows 2% Tryptone (Difco), 0.5% yeast extract (Difco), 0.5% peptone (Difco), 0.5% glucose, 0.005% Tween 80 (Nutritional Biochemicals Corp.), and 2% agar (Difco) in a filtered or centrifuged fourfold dilution (with water) of tomato juice (containing no preservatives). The medium is adjusted to pH 5.5 with HC1 before adding agar. 

Erwinia herbicola NCIMB 12126 was obtained from the National Collection of Industrial and Marine Bacteria (Aberdeen, UK), HEPES buffer sachets and magnesium acetate were obtained from Sigma (Poole, UK), adenylate kinase assay kits were obtained from Acolyte Biomedica (Salisbury, UK), sterile tissue culture grade distilled water was obtained from Gibco (Paisley, UK), L-broth and tryptone soya agar plates were obtained from Oxoid (Basingstoke, UK). 

The plate counts at To were estimated by diluting the neat broth culture 1 in 10 with Neutralised Peptone Water (NPW) containing per L 1.0 g Bacteriological Peptone (Oxoid, L37), 8.8 g Sodium Chloride (May Baker), 3.0 g Amisol 910 (Degussa) and 30.0 g Tween 80 (BDH, 560234H) and then decimally with Phosphate Buffered Saline (Oxoid). For biocide treated samples 1 mL was diluted in 9 mL of NPW, mixed and allowed to stand for 5 min (for neutralisation of the biocide). The solution in NPW was diluted decimally (0.1 mL in 0.9 mL) in PBS. Appropriate dilutions (0.1 mL) were plated out on Tryptone Soya Agar Plates (bioMerieux) and incubated at 37 °C for 24 h. 

LB agar (5g bacto-yeast extract, lOg bacto-tryptone, 10 g NaCl, 15 g granulated agar water added to 1L autoclaved). 

LB-Amp plates Dissolve 10 g tryptone, 5 g yeast extract, 10 g NaCl, and 15 g agar in 1L deionized water and sterilize by autoclaving. Add lmL of l,000x (100mg/mL) ampicillin stock to cool (<50°C), mix and pour plates. 

Inoculate a sample of the bacterium from an agar slope into a medium of 9 mL Tryptone Soya Broth or Nutrient Broth and incubate overnight in an orbital shaker at 37°C. 

Sunesson A.L., Nilsson C.A., Carlson R., Blomquist G. and Andersson B. (1995d) Influence of temperature, oxygen and carbon dioxide levels on the production of volatile metabolites from Streptomyces albidoflavus cultivated on gypsum board and tryptone glucose extract agar. In Sunesson A.L., Volatile metabolites from microorganisms in indoor environments-sampling, analysis and identification. Thesis, Umea University, Sweden. 

Coded and dated, sterile, tryptone soya agar plates should be exposed for two hours at all test sites within the isolator. These should be incubated in accordance with a written SOP at the appropriate temperature for up to five days, or as otherwise chosen by the microbiologist... 

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