Tryptone

An incubated culture of Actinomyces antibioticus was prepared using a medium consisting of 1% tryptone-peptone, 0.5% starch, 0.2% K HPO, 0.2% NaCI and 0.25% agar in distilled water, grown at a temperature of approximately 25° to 35°C, the incubation being complete after 6 to 10 days. 50 liters of this incubated culture are extracted approximately six times with ether, using 20 liters of ether for each extraction. 

Reports on a few other desulfurization organisms also exists, including Nocardia [98], S. acidocaldarius [5,99], Arthrobacter [100], R. toruloides, [101], C. elegans [102], Klebsiella spp. [103], and Alcaligenes xylosoxydans strain deposited with the ATCC under PTA-4669 [104], A S. acidocaldarius species was reported to desulfurize DBT and produce sulfate [5], A similar strain was later used to study the DBT desulfurization [99] in complex media using tryptone however, the authors reported that the strain was not active when grown on sucrose. It was apparently inhibited by DBT and also affected by the interface in oil-water mixtures [105],... 

From Oxoid peptone tryptone yeast extract. 

Luria-Bertani (LB) medium tryptone (25 g), yeast extract (13 g), NaCl (25 g)... 

Bacto-tryptone (1 g), bacto-yeast extract (0.5 g) and NaCl (1 g) were dissolved in 95 mL of deionized water and the pH was adjusted to 7.0 with 5 m NaOH. The volume was adjusted to 100 mL with deionized water. A portion of the resulting solution (5 mL) was placed in a 20 mL test tube with a poromeric silicone plug and the rest was placed in a 500 mL Sakaguchi flask with a poromeric silicone plug. The media were sterilized by autoclaving (121 °C, 20 min). 

Tryptone (5 g), yeast extract (2.5 g) and NaCl (5 g) were dissolved in distilled water, the volume was adjusted to 500 mL and then autoclaved (20 min, 120 °C). A small portion of this Luria-Bertani (LB) medium (10 mL) was placed into a sterile 100 mL shake flask and ampicillin and chloramphenicol solutions were added (LBamp+cm) to final concentrations of 100 p,g mL and 20 p.g mL respectively. The solution was inoculated with E. coli JM109 pGro7 pJOE4072.6 and shaken overnight at 37 °C and 200 rpm. This overnight culture (2 mL) was used to inoculate 200 mL LBamp+cm in a... 

Luria-Bertani (LB) medium (tryptone peptone 10 g yeast extract, 5 g L , NaCl 5 g... 

Test Procedure. The test organisms were subcultured onto Tryptone Soya Agar (TSA) slopes and incubated at 37 °C for 24 hours. After incubation for each inoculum, 6 ml sterile distilled water (SDW) was added to each slope in turn, the organisms washed off and the resultant suspension homogenised. A 1 ml aliquot of the suspension was added to 100 mis SDW and homogenised to form the inoculum. 

Materials for media (Bacto Tryptone, Bacto Yeast Extract and Difco SOB Medium, agar powder and NaCl) and antibiotics (ampicillin sodium and kanamycin sulfate). 

The clones were transferred into 96 deep-well microtiter plates filled with 100 pL LB medium (10 g NaCl, 5g yeast extract, 10 g trypton) supplemented with 50 pg mL kanamycin. After overnight shaking (600 rpm) at 37 °C, 600 pL LB medium containing kanamycin (50 pg mL ) were added. After an additional... 

Total aerobic viable count pour 1 ml into plate containing 25 ml of tryptone glucose extract agar or TSA half concentration. Incubate at 22°C for 5 days. 

Swab transportation tryptone saline, peptone water, enriched buffered gelatine, buffered saline, buffered gelatine, and brain-heart infusion are generally recommended media that may be used for swab transportation. For RCS + testing agar strip TC (BIOTEST) or M air T air sampler for level I is used. 

The swab, generally composed of a stick with an absorbent extremity, is immersed in 3 ml of an appropriate diluent (tryptone saline with suitable neutralizer) before sampling. 

Inoculate the surface of tryptone soya agar slant for bacteria and Sabouraud dextrose agar slant for fungi from recently revived stock culture of each of the test microorganisms. 

Promptly pour into each petri dish about 15 to 20 ml of sterile melted tryptone soya agar medium previously melted and cooled to approximately 45°C. 

Note TSA = tryptone soya agar SDA = Sabouraud dextrose agar. 

Rogosa-type medium (60) made as follows 2% Tryptone (Difco), 0.5% yeast extract (Difco), 0.5% peptone (Difco), 0.5% glucose, 0.005% Tween 80 (Nutritional Biochemicals Corp.), and 2% agar (Difco) in a filtered or centrifuged fourfold dilution (with water) of tomato juice (containing no preservatives). The medium is adjusted to pH 5.5 with HC1 before adding agar. 

Among the first to describe a procedure for preparing frozen concentrated cultures were Foster (1962) and Lamprech and Foster (1963). In their process, they grew S. lactis or S. lactis subsp. diacetylactis separately at 25 °C in a tryptone-yeast extract-glucose-magnesium phosphate medium. Cells early in the maximum stationary phase (10 to 15 hr of incubation) were recovered by centrifugation, resuspended... 

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