Brain-heart infusion

Cells (C), or supernatant liquor (S). b YCV, Yeast nitrogen base-Casamino acids-Vitamins. M, Synthetic medium. d BHI, Brain-heart infusion. Sab, Sabouraud.1 B, Mariat s medium. 

Fig. 24.—13C-N.m.r. Spectrum of a Galactofuranose-Containing Polysaccharide from Sporothrix schenckii Grown on a Medium Containing Brain-Heart Infusion. (Solvent, DsO temperature, 70° chemical shifts expressed as 8C, relative to external tetramethyl-silane.)...

Swab transportation tryptone saline, peptone water, enriched buffered gelatine, buffered saline, buffered gelatine, and brain-heart infusion are generally recommended media that may be used for swab transportation. For RCS + testing agar strip TC (BIOTEST) or M air T air sampler for level I is used. 

GRAM POSITIVE BACTERIA B.subtilis Brain Heart Infusion 37°C, 24h... 

GRAM NEGATIVE BACTERIA S.marcescens Brain Heart Infusion 37°C,24h... 

In the morning, BHI (brains-heart infusion) nutrient broth was inoculated with bacteria, from an overnight culture or agar, to be used within 4 h. Within that incubation time, Mueller-Hinton (MH) plates were allowed to reach room temperature. 

This stock solution is conveniently stored frozen at — 20°C in 20 ml and 80 ml amounts after checking for contamination using 1) Saboraud fluid medium at 31°C for 1 week (Appendix 4) 2) brain heart infusion broth at 37°C for 1 week (Appendix 4). (One check of each should contain calf serum). 

Various other broths for detecting bacterial and fungal contamination include brain heart infusion broth, tryptose phosphate broth and trypticase soy broth. These should be made up as per manufacturers (Oxoid Ltd. or Difco Labs. see Appendix 3) instructions, but some procedures are given in Appendix 4. 

Both batches of virus should be tested for bacterial contamination (Chapter 9) by inoculating an aliquot of virus into brain-heart infusion broth (leave at 37° C for one week) and into Saboraud fluid medium (32°C for one week) (Appendix 4). 

Although this procedure is necessary for production of samples of pure virions it is usually unnecessary if a viral preparation is only required for subsequent infections. In such circumstances the cells should be harvested aseptically and processed to step e, omitting the DNase and RNase. The debris from the disrupted cells is pelleted at 15,000g for 30 min and the supernatant used as a source of virus. It should be tested for bacterial contamination with brain-heart infusion broth and Saboraud fluid medium (Appendix 4). The virus should be stored at -70°C at about 1010 p.f.u./ml. 

Dissolve 37.0 g of brain heart infusion broth powder (Oxoid, Basingstoke) in 1 1 of distilled water. 

The MICs were determined on brain-heart infusion agar containing a gradient of the test antibiotic. Cultures with 10 bacteria/ml were streaked on the gradient. The MICs were read after overnight incubation at 37°C... 

Minimal inhibitory concentrations (MIC) determined in brain-heart infusion broth containing 10 cells/ml. Minimal bactericidal concentrations (MBC) determined by subculture into broth. Figures given are averages of two tubes except that the readings from four tubes were averaged for the E. coli data. 

Bacto brain heart infusion agar (Difco)... 

A dialysis bag containing 900 ml of 0.9 % NaCI in a total volume of 41 brain heart infusion (BHI, Difco) is inoculated with 100 ml of an overnight BHI culture of C. difficile strain VPI 10463. 

Preparation of culture medium and production of culture supernatants containing SLO. Seeding cultures of Streptococcus pyogenes, Richards strain, are prepared in Brain-Heart-Infusion medium by overnight incubation at 37°C. For long term storage of the strain, the culture is supplemented to 50% (by volume) with sterile glycerol and kept at -70°C. 

Brain heart infusion Bacto Profuse-Peptone No. 3 Ultrafiltration membrane (cut off 30kDa) Thiopropyl-Sepharose 6B Alkyl-Superose HR 10/110 column... 

When ultrasound was applied at moderate temperatures—below those used in pasteurization—to brain/heart infusion contaminated with Salmonella ty-phimurium, a reduction of contamination of 99% was observed [43], Once again the enhancement to the efficiency of the process induced by sonication decreased as temperature increased. Reductions in contamination were also observed in the similar treatment of skimmed milk. 

Typically, the H. pylori isolates were frozen immediately after isolation (-70 C). Cultures were subsequently thawed and plated onto brain heart infusion agar (1.2%, w/v) plates supplemented with 0.5% (w/v) yeast extract and 0.5% (v/v) fetal bovine sera. The H. pylori strains were allowed to grow for 3 days under microaerobic conditions at 37 C, subcultured into brain heart broth, and allowed to grow for an additional 3 days under the same conditions with agitation. 

Two types of rich media (brain heart infusion (BHI) and homogenized chicken meat media (CMM)) containing various concentrations of NaCl, glycerol, or raffinose and various water contents to obtain a values 0.86 to 0.99 at 25°C have been considered related to S. aureus growth. The CMM media was more viscous than the BFH media at given moisture content, probably because of the presence of unhydrolyzed proteins. The presence of raffinose also led to a higher viscosity than the presence of NaCl or glycerol. 

Enrichment broths have consisted of brain heart infusion (BHI) with cysteine and yeast extracts (Songer et al., 2009) and Oxoid C. difficile medium without agar (Rodriguez-Palacios et al., 2009 Weese et al., 2010 Table 3.1). These enrichment media may be supplemented with cefoxitin ( 16 pg/ml) and cycloserine ( 500 gg/ml) or moxalactam (32 pg/ml) and norfloxacin (12 gg/ml). Alcohol shock and subculture on commercial solid media described above follow incubation under anaerobic conditions for 2-12 days. 

Bacillus subtilis cells were grown in 1 liter brain heart infusion broth for 36 hours, centrifuged,and the cells were washed 2 times with sterile phosphate buffer Ten ml of the washed cell suspension was added to each flask which contained 0.5 gms of carrier. After 3 hours of contact time, the carriers were washed 3 times and maintained at 8°C overnight. 

Plastic pestles that conform to the interior of a 1.5-mL microfuge tube. Brain-heart infusion (BHI) broth (Difco, Sparks, MD) and sterile PBS. 

MgS04, glycerol, glucose, Luria-Betani (LB) broth, trypticase soy broth (TSB) (Difco, Detroit, MI), and brain heart infusion (BHI) media (Becton Dickinson, Franklin Lakes, NJ). 

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