Bacto yeast extract

Bacto-tryptone (1 g), bacto-yeast extract (0.5 g) and NaCl (1 g) were dissolved in 95 mL of deionized water and the pH was adjusted to 7.0 with 5 m NaOH. The volume was adjusted to 100 mL with deionized water. A portion of the resulting solution (5 mL) was placed in a 20 mL test tube with a poromeric silicone plug and the rest was placed in a 500 mL Sakaguchi flask with a poromeric silicone plug. The media were sterilized by autoclaving (121 °C, 20 min). 

Isolation of Plasmid Enriched DNA. The three strains of B. megaterium were cultured in yeast-soy broth (4% Bacto-yeast extract and 1.6% Bacto-soytone from Difco Laboratories, Detroit, pH 7.2). Plasmid DNA from each strain was isolated and purified by cesium chloride-ethidium bromide equilibrium density centrifugation following the procedure of Carlton and Brown ( ). Subsequent gel electrophoresis (0.5% agarose) revealed the presence of some chromosomal DNA contamination. 

Materials for media (Bacto Tryptone, Bacto Yeast Extract and Difco SOB Medium, agar powder and NaCl) and antibiotics (ampicillin sodium and kanamycin sulfate). 

LB broth 10 g/L Bacto-tryptone, 5 g/L Bacto-yeast extract, 10 g/L NaCl, adjusted to pH 7.0 with NaOH and sterilized by autoclaving. 

THY broth Todd-Hewitt broth (Beckton Dickisnson, MD, USA) supplemented with 1% Bacto yeast extract (Beckton Dickisnson, MD, USA). 

Streptomyces sp. NRRL 1143 Bacto yeast extract Enzyme hydrolyzed casein... 

SOB medium 20g/L bacto-tryptone (Difco Laboratories), 5g/L bacto-yeast extract (Difco Laboratories), 0.5 g/L NaCl, 16.6 g/L KC1. 

Luria broth—Luria broth Prepare five 100-ml and five 10-liter solutions containing 10 g/liter Bacto-Tryptone, 5 g/liter Bacto-Yeast extract, 5 g/liter NaCl, 0.5 g/liter antifoam agent (e.g., Polyglycol P-2000), and 1 g/liter glucose (autoclave separately). Autoclave the main solution and separate solution of concentrated glucose for 20 min at 105 to 110°C and cool use sterile conditions to add the glucose solution to the main solution and use within 24 hr. 

Bacto Tryptone Bacto Yeast Extract SP-Sepharose fast flow... 

LB medium (for 1 L) 10 g bacto-tryptane, 5 g bacto yeast extract, 10 g NaCl in water, adjust to pH 7.0 with 5 M NaOH. 

YT media (10 g bacto-yeast extract, 16 g bacto-tryptone, 5 g NaCl water added to 1 L and pH adjusted to 7.0 with NaOH autoclaved). 

LB agar (5g bacto-yeast extract, lOg bacto-tryptone, 10 g NaCl, 15 g granulated agar water added to 1L autoclaved). 

SOC media (5g bacto-yeast extract, 20g bacto-tryptone,... 

YPD, 6 liters 1% (w/v) Bacto-yeast Extract, 2% (w/v) Bacto-peptone, 2% (w/ v) glucose. Autoclave 1-liter volumes for the minimum exposure time necessary for sterilization, and begin cooling immediately after the exhaust cycle. We use Fernbach flasks. 

X TY medium dissolve 16 gBacto tiyptone, 10 g Bacto yeast extract, and 5 g NaQ in 800 ml of distilled water, adjust to pH 7.0 with 1 M NaOH, adjust the volume to 1 litre with distilled water, sterilize by autoclaving... 

YPAD liquid medium 2% (w/v) bacto peptone, 1% (w/v) bacto yeast extract, 2% (w/v) dextrose, lx adenine hemisulfate solution. Autoclave or filter sterilize and store at 4°C. 

Luria-Bertani (LB) medium sodium chloride (NaCl, 10 g/L), Bacto tryptone (10 g/L), and Bacto yeast extract (5 g/L). Add 15 g/L Bacto agar to LB medium to prepare LB agar for plating. Dissolve in ddH O and autoclave. 

Luria-Bertani (LB) with carbenicillin or LB with chloramphenicol For 1 L of medium, add 10 g of sodium chloride (NaCl), 10 g of Bacto Tryptone, and 5 g of Bacto Yeast Extract. Dissolve in ddH O, adjusting the volume to 1 L. Autoclave and allow medium to cool down below... 

YPD plates 1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, and 2% Bacto agar. 

YPAD medium 1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, and 0.006% adenine sulfate. For optimal yeast growth, make this medium from components, not a premixed YPD powder, and filter-sterilize. 

SOS solution 1 M sorbitol, 6.5 mM CaClj, 0.25% Bacto yeast extract, 0.5% Bacto peptone. 

Potassium acetate Bacto yeast extract Glucose Bacto agar... 

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