Trichomonas species

Thong, K. W. and Coombs, G. H. (1987) Trichomonas species homocysteine desulphurase and serine sulphydrase activities. Exp. Parasitol. 63 143-151. 

Catalase is present in the cytoplasm of T. foetus (26) but absent from other Trichomonas species, Giardia lamblia (27) and E. histolytica (5). 

The metabolism of metronidazole has been studied in both species of pathogenic trichomonads that are responsible for sexually transmitted urogenital diseases in humans (Trichomonas vaginalis) and cattle (Tritrichomonas foetus). Metronidazole enters the cell as an inactive prodrug by... 

Trichomonad flagellates seemed to be a good first choice. The human pathogen Trichomonas vaginalis and the cattle pathogen Tritrichomonas foetus were much-studied species available in bacterium-free cultures and were thus amenable to biochemical and cell fractionation studies, approaches extensively practiced by our group. The available physiological data showed that the respiration of these species was not of mitochondrial type, because it could not be inhibited with cyanide and other mitochondrial inhibitors. Furthermore no cytochromes were detected in these trichomonads (Ryley 1955). 

Metronidazole is DOC for most infections caused by Entamoeba histolytica, Giardia species, and Trichomonas vaginalis. 

Miconazole [22916 47-8] has a potent antifungal action against dermatophytes and Candida species. It is also active against gram-positive bacilli and cocci. In vitrOy it is also effective against Trichomonas, Leishmaniay and Plasmodium. Miconazole is only moderately absorbed in the digestive tract. After intravenous administration, it is rapidly metabolized. 

When alanine is a major end-product of glucose catabolism, substantial amounts of nitrogenous compounds must be available to contribute the amino group. In a number of species alanine formation is accompanied by proteolysis which would release a pool of amino acids from which the nitrogen could be derived. In G. lamblia and Trichomonas vaginalis arginine catabolism is a likely provider of nitrogen for alanine synthesis. The efflux of alanine from G. lamblia is due to an alanine antiport which is also responsible for alanine uptake and acts to maintain a balance between intracellular and extracellular alanine concentrations (36). 

The simple purine salvage scheme of the mucosal pathogens Giardia, Trichomonas, and Entamoeba species) with the lack of crossover from adenine to guanine metabolism means that there is an absolute requirement for each of these purines. This suggests that inhibition of either pathway will inhibit the organisms growth (Chapter 6). 

Trichomonas and Trichomastix With one exception, all the species of these genera have been cultured only on complex media (beef broth, blood serum, egg, etc.). Even in the case of Trichomonas foetus (Weiss and BalP), which certainly needs confirmation, the medium contained extracts of plant materials. For optimum growth unknown substances had to be added in enzymatically digested proteins or serums. 

Antifungal - in vitro (Agar plate) against Rhizopus oryzae, R. oligosporus, Trichoderma species at 200.0 pg/disc. 162 Antitrichomonal - in vitro (Agar plate, Trichomonas vaginalis) at 10.0 pg/disc. 163... 

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