Potatoes transgenic

Transgenic potatoes that express an Erwinia pectate lyase isoenzynne... 

Such observations led to the hypothesis that regulated expression of PL3 in transgenic potatoes could contribute to enhanced tissue disintegration during storage or thermal treatment. This report addresses the properties of several independent transgenic potato plant lines that express PL3, either constitutively or tuber-specific. We also describe the results obtained following application of Erwinia bacteria to wounded potato tubers. 

The development of a by-production system could further minimize the cost of such plant-derived products. For example, potatoes are the raw material for the production of technical grade starch. During this process, soluble proteins are separated by heat treatment and sold as animal fodder. Recombinant proteins could be produced in transgenic potato tubers as a by-product of starch extraction, and this would be useful for proteins produced in large amounts with a low commercial impact, like structural fiber proteins. 

Chakraborty S, Chakraborty N and Datta A. 2000. Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus. Proc Natl Acad Sci USA 97 3724-3729. 

Application to Study of Starch Metabolism in Transgenic Potato Tubers... 72... 

SWEETLOVE, L.J., BURRELL, M.M., AP REES, T., Starch metabolism in tubers of transgenic potato (Solanum tuberosum) with increased ADPglucose pyrophosphorylase, Biochem. J., 1996, 320,493-498. 

ZRENNER, R., SALANOUBAT, M., SONNEWALD, U., WILLMITZER, L., Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.), Plant J., 1995, 7, 97-107. 

Edible food sources have been tested to deliver vaccines orally for example, transgenic potato tuber-based vaccines have been developed. Other food sources, such as bananas, tomatoes, and corn, are being tested in laboratories (see Section 11.12). Mucosal vaccines, utilizing genetically modified enterotox-ins, are delivered intranasally. Research in this area has to ensure the safety aspect of using enterotoxins. 

Of the 14 volunteers for the Phase I study, 11 were given the transgenic potatoes containing the toxin as vaccine and three had ordinary potatoes. Blood and stool samples were collected from the volunteers to evaluate the vaccine s ability to stimulate both systemic and intestinal immune responses. Ten of the 11 volunteers who ingested the transgenic potatoes had fourfold rises in serum antibodies at some point after immunization, and six of the 11 developed fourfold rises in intestinal antibodies. The potatoes were well tolerated and no one experienced serious adverse effects. 

Shulga NY, Rukavtsova EB, Krymsky MA, Borisova VN, Melnikov VA, Bykov VA, Buryanov Yl. (2004) Expression and characterization of hepatitis B surface antigen in transgenic potato plants. Biochemistry 69 1158-1164. 

Stobiecki, M. et al.. Monitoring changes in anthocyanin and steroid alkaloid glycoside content in lines of transgenic potato plants using liquid chromatography/mass spectrometry. Phytochemistry, 62, 959, 2003. 

Martin-Alonso, J.M., Castanon, S., Alonso, R, Parra, E, and Ordas, R. (2003). Oral immunization nsing tuber extracts from transgenic potato plants expressing rabbit hemorrhagic disease virus capsid protein. Transgenic Res. 12 127-130. 

Ohya, K., Matsumura, T., Itchoda, N., Ohashi, K., Onuma, M., and Sugimoto, C. (2005). Ability of orally administered IFN-alpha-containing transgenic potato extracts to inhibit Listeria monocyogenes infection. J. Interferon. Cytokine Res. 25(8) 429 66. 

Potato Potato tubers have been used extensively as the crop of choice in over three separate human cliiucal trials so far and have been utilized to produce vaccines, human serum albumin, TNF-a and various antibodies. However, since transgenic potatoes expressing the desired biopharmaceutical must be delivered in an uncooked form, they have been known to... 

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