Transactivation response element

Daelemans, D., Schols, D., Witvrouw, M., Rannecouque, C., Hatse, S., Door-EN, S.V., Hamy, F., Klimkait, X, Clercq, E.D., and Vandamme, A.-M. A second target for the peptoid Xat/transactivation response element inhibitor CGP64222 Inhibition of human immunodeficiency virus replication by blocking CXC-chemo-kine receptor 4-mediated virus entry. 

Transactivation-response element of HIV-1 [19] hnine exchange <230 SELEX, HPLC, and MALDI-TOFMS... 

HIV Tat can cross nuclear membranes and localize in the nucleus, where it binds to TAR (transactivating response element), a stem-loop RNA found on... 

HIV human immune deficiency virus HPV human papillomavirus HSV herpes simplex virus ICAM intercellular adhesion molecule IL interleukin IL-la interleukin 1 a LNA locked nucleic acid mRNA messenger RNA NK natural killer PKC protein kinase C ply rC poly ribocytosine PNA peptide nucleic acid poly rl poly ribinosine PTHrP parathyroid hormone-related peptide thermal melting temperature change in relative to DNA TAR transactivator response element VSV vesicular stomatitis virus... 

A. Litovchick, A. G. Evdokimov, and A. Lapidot, Argitrine-aminoglycoside conjugates that bind to HIV transactivation responsive element RNA in vitro, FEBS Lett., 445 (1999) 73-79. 

The effects of divalent Mg " on the conformation and dynamics of the stem-loop transactivation response element (TAR) RNA from HIV-1 have been... 

One example is CGP64222, originally designed as a mimic of the transactivation response-element-binding domain of the HIV tat protein (TAR) has been shown to completely block tat binding to TAR. Subsequently, it has also been shown to block an early step in the life cycle of HIV by binding to CXCR4 selectively (Daelemans et al., 2000). 

This was allowed to interact with a stoichiometric amount of a 27-nucleotide form of the transactivation-response element (TAR) RNA hairpin loop of HIV-1. Reductive amination of the DCL was applied and the increased formation of one of the different combinations could be shown. In a later proof-of-principle study [62], the concept was extended and the combinatorial SELEX (Systematic Evolution of Ligands by Exponential Enrichment) method to DNA/RNA aptamers was directly combined with the reversible imine formation. The process led to the selection of modified aptamers, conjugated to a range of non-nucleotidic aldehydes. The method proved successful for the selection of small-molecule conjugated RNA aptamers that bind tightly to the TAR element of HIV-1 (Figure 5.27). 

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