Swab plating

Swab plating Metals Various 0.01-6 0.25-150 Special applications... 

Brush plating is a special technique which dispenses with a container and uses a swab soaked in electrolyte applied to the work. In jet plating a stream of electrolyte is applied to the cathode. Both are methods of selective plating, applying an electrodeposit to only a part of an article. Little has been published about the techniques or the properties of coatings they produce. 

Nishannon A. Pokja M.S. (1977) Comparative studies ofmicrohial contamination of surfaces hy the contact plate and swab methods. 7,4/7/)/ Bacterial, 42, 53-63. 

After the experiments, the Prototype Unit was disassembled and the component parts were individually swabbed with sterilized cotton wools (4 cm2). Each samples were stored in 1 ml sterilized distilled water in an eppendorf tube. 50 pi of sample was transferred to TSA and MEA plates. The TSA plates were incubated at 37 °C for 24 h and bacterial colonies were counted. The number of fungal colonies was determined from the MEA plates after incubating at 30 °C for 5 days. The results show no viable bacterial and fungal colonies were present in the interior parts of the Prototype Unit. Viable colonies are found on the external surface of the unit. This suggests that air passing through the Prototype Unit was sterilized by the action of the formulated catalyst. 

Cotton swabs or RODAC plate (nutrient agar culture medium)... 

Contact plate areas vary from 24 to 30 cm. When swabbing is used in sampling, the area covered should be greater than or equal to 24 cm but no larger than 30 cm. ... 

The use of contact plates is the recommended technique. The RODAC plate method is the simplest, but it is useful only for flat surfaces. Swab samples afford examination of corners, crevices, and other area inaccessible to RODAC plates. 

Repeat this for each test organism. For positive control use sterile DW instead of sanitizer solution. For negative control use sterile strips. Swab each strip thoroughly with a premoistened Ca alginate swab and place in Lecithin broth (or other suitable neutralizer). Vortex for 30 sec, then perform the usual pour plate method. 

Level 1 Supporting areas immediately adjacent level 1 Potential product/container contact areas Other support areas (class 100,000) Other support areas to aseptic processing areas but no product contact (class 100,000 or lower) 1 CFU/plate or swab 3 CFUs/plate or swab All sites 10 CFUs/plate Floor 20 CFUs/plate All sites 25 CFUs/plate Floor 30 CFUs/plate All sites 50 CFUs/plate Floor 100 CFUs/plate... 

Two days before testing incubate in inverted position a sufficient number of RODAC plates (or contact plates), swabs, agar strips, and plates for fingerprints. 

To minimize disruptions to critical operations, surface sampling should be performed at the stop or the end of operations. Surface sampling may be accomplished by the use of contact plates or by the swabbing method. 

The area to be swabbed is dehned using a sterile template of appropriate size. In general, it is in the range of 24 to 30 cm. The swab is then rinsed with vortex and the contents plated on TSA plates. 

Count the number of microbial colonies on each plate. The microbial estimates are reported per contact plate or per swab. Examine the types of colonies and submit the representative colonies for identification. Mold does not carry special status relative to bacteria. Any significant shifts in type or number require action — regardless of mold vs. bacteria. 

Class 100 1 CFU/m3 1 CFU/plate or swab 1 CFUs/ 10 fingers 2 CFUs/ plate... 

Environmental control monitoring SOP No. All result of active air sampling settle plates RODAC plates, swabs and operator s gloved hand plates within the acceptance limit... 

Sampling contact plates and swabs shall be used. 

For very rapid on-farm screening of antibiotic residues in animal tissues, the swab test on premises (STOP) has been widely employed (86). This test involves inserting a cotton swab directly into meat tissues, allowing it to absorb tissue fluids. The swab is the removed and placed on a test plate to be incubated with Bacillus subtilis ATCC 6633 spores at 29 C overnight for evidence of inhibition around the swab. 

Note 2 Fungi spores from an active culture were suspended in 1 ml sterile 0.85 NaCl, then lawned on 150 mm Mueller-Hinton agar plates with a sterile cotton swab... 

Culturing of Test and Control Samples. Both control and treated spore strip samples were cultured at 37 °C in trypticase soy broth for 48 hr to determine kill. Swab samples, taken from sections of the surface of the muddy-moldy pages before and after exposure to ethylene oxide, were placed in 1 mL of 0.001M phosphate buffered saline solution at pH 7.0 for plating on appropriate media. These samples were taken from a 6.45-cm2 section. 

To determine the effect of adding moisture to dried contaminated pages, 1 mL of the phosphate buffered saline solution at pH 7.0 was added to a section of each of several muddy-moldy pages before treatment with ethylene oxide. Swabs were taken from these selected areas before and after the gaseous treatment. Duplicate plates were made of all samples. 

Discard the solutions and precipitates into the container provided by your teacher. Rinse the plate with water, and clean the wells using a cotton swab. 

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