Surface-Enhanced Resonance Raman Scattering SERRS

Surface-Enhanced Resonance Raman Scattering (SERRS) [Pg.325]

The technique of in situ Raman spectroscopy at metal electrodes has been recently reviewed by Pettinger. Adsorbed monolayers of proteins have been subjected to interfacial spectroelectrochemical characterization us- [Pg.325]

Since little is known about the interfacial behavior of enzymes other than glucose oxidase (GOD) at an electrode, Taniguchi et examined the electron-transfer reactions and adsorption behavior of several flavoen-zymes by fluorescence and SERRS measurements. They found that the intensities were useful in determining the location of flavin adenine dinucleotide (FAD) in the flavoenzymes, which suggested possible interfacial structures of these flavoenzymes. [Pg.326]

Surface-enhanced resonance Raman scattering (SERRS), 21 327-328 advantage of, 21 329 Surface Evolver software, 12 11 Surface excess, 24 135, 136 Surface extended X-ray absorption fine structure (SEXAFS), 19 179 24 72 Surface filtration, 11 322-323 Surface finish(es). See also Electroplating in electrochemical machining, 9 591 fatigue performance and, 13 486-487 Surface finishing agents, 12 33 Surface force apparatus, 1 517 Surface force-pore flow (SFPF) model,... [Pg.911]

Another method for assaying the activity and stereoselectivity of enzymes at in vitro concentrations is based on surface-enhanced resonance Raman scattering (SERRS) using silver nanoparticles (116). Turnover of a substrate leads to the release of a surface targeting dye, which is detected by SERRS. In a model study, lipase-catalyzed kinetic resolution of a dye-labeled chiral ester was investigated. It is currently unclear how precise the method is when identifying mutants which lead to E values higher than 10. The assay appears to be well suited as a pre-test for activity. [Pg.30]

Li et al. have performed a comparative study on the surface-enhanced resonance hyper-Raman scattering and surface-enhanced resonance Raman scattering (SERRS) of dyes adsorbed on Ag electrode and Ag colloid [210]. [Pg.933]

S S CONTENTS Preface, C. Allen Bush. Methods in Macromo-lecular Crystallography, Andrew J. Howard and Thomas L. Poulos. Circular Dichroism and Conformation of Unordered Polypeptides, Robert W. Woody. Luminescence Studies with Horse Liver Dehydrogenase Information on the Structure, Dynamics, Transitions and Interactions of this Enzyme, Maurice R. Eftink. Surface-Enhanced Resonance Raman Scattering (SERRS) Spectroscopy A Probe of Biomolecular Structure and Bonding at Surfaces, Therese M. Cotton, Jae-Ho Kim and Randall E. Holt. Three-Dimensional Conformations of Complex Carbohydrates, C. Allen Bush and Perse-veranda Cagas. Index. [Pg.306]

Surface-enhanced resonance Raman scattering (SERRS) has also been achieved using silver colloid aggregates produced in situ in the chip. This method was used to detect an azo dye, 5-(2,-methyl-3,5,-dinitrophenylazo)quinolin-8-ol, which is a derivative of the explosive, TNT. With this method, it was possible to detect 10 iL of 10 9 M dye (or 10 fmol). This represented a 20-fold increase in sensitivity over that achieved using a macro flow cell [739]. [Pg.211]

Further enhancement can be observed if the exciting light additionally couples into an electronic transition of the adsorbate (surface-enhanced resonance Raman scattering, SERRS). In this case, enhancement factors of... [Pg.51]

Surface enhanced resonance Raman scattering (SERRS) is an analytical technique with several advantages over competitive techniques in terms of improved sensitivity and selectivity. We have made great progress in the development of SERRS as a quantitative analytical method, in particular for the detection of DNA. However, one of the main advantages over fluorescence and other optical detection techniques is the ability to multiplex. [Pg.353]

Surface enhanced resonance Raman scattering (SERRS) [1-3] is a highly sensitive technique, so sensitive in fact that single molecule detection has previously been reported [4, 5]. It is a very attractive technique for the detection of biomolecules because it produces molecularly specific spectra which make it feasible to easily identify the components of a mixture in a single analysis without extensive separation procedures [6]. [Pg.354]

Faulds K, Fruk L, Robson DC, Thompson DG, Enright A, Smith WE, Graham D (2006) A new approach for DNA detection by SERRS. Faraday Discuss 132 261-268 Faulds K, Smith WE, Graham D (2004) Evaluation of surface enhanced resonance Raman scattering (SERRS) for highly sensitive and quantitative DNA analysis. Anal Chem 76 412-417... [Pg.377]

Graham D, Mallinder BJ, Whitcombe D, Smith WE (2001) Surface enhanced resonance Raman scattering (SERRS) - a first example of its use in multiplex genotyping. ChemPhysChem 2(12) 746-748... [Pg.377]

Faulds, K., Jarvis, R., Smith, W.E., Graham, D., and Goodacre, R. (2008) Multiplexed detection of six labelled oligonucleotides using surface enhanced resonance Raman scattering (SERRS). Analyst, 133, 1505-1512. [Pg.319]

Using biomolecules with chromophoric groups the Raman bands are both resonance (RRS) — and surface enhanced (RRS + SERS = SERRS). Instead of the usual term SERS, the Raman effect is, in this case, called surface enhanced resonance Raman scattering (SERRS). SERRS spectroscopy was first applied to biochemistry of heme chromophores by Cotton et al. Since then, SERS and SERRS have been extended to systematic investigations of biomolecules in the adsorbed state... [Pg.3]

K. Kneipp, Y. Wang, R. R. Dasari, and M.S. Feld, Approach to Sin -Molecute Detection Using Surface-Enhanced Resonance Raman-Scattering (SERRS) - a Study Using Rhodamine 6g on Colloidal Silver, Appl. Spectrosc. 49, 780 (1995)... [Pg.413]

Prochazka et al. demonstrated the suitability of surface-enhanced resonance Raman scattering (SERRS) spectroscopy to monitor silver coordination of free base 5,10,15,20-tetrakis(l-methyl-4-pyridyl) porphyrin adsorbed on the Ag colloidal surface. The approach was based on factor analysis (FA) which allows the analysis of the time development of SERRS spectra in terms of varying content of particular porphyrin forms. Their SERRS spectra can be isolated even if none of them is present singularly in any SERRS spectmm. Depending on the chemical properties of the molecular systems, SERRS spectra of two different porphyrin metalated forms have been obtained Ag+ and Ag metalated. The latter form was found to be evoked by pre-treatment of the laser-ablated Ag colloids by the thiosulphate ions. The metalation kinetics obtained show a substantially slower metalation by Ag than that by Ag+ cations. [Pg.772]

SURFACE-ENHANCED RESONANCE RAMAN SCATTERING (SERRS) SPECTROSCOPY OF BACTERIAL MEMBRANES THE FLAVOPROTEINS. [Pg.1861]

Surface-Enhanced Resonance Raman Scattering (Serrs) Spectroscopy of Bacterial... [Pg.3821]

Surface-enhanced resonance Raman scattering (SERRS) is a very sensitive technique with many applications, especially in forensic analysis. SERRS was successfiil in differentiating the majority of the 26 inks examined in an experiment designed to show that the method is less invasive than TLC and can be used coupled with an HPTLC plate. [Pg.2189]

K Kneipp, Y Wang, RR Dasari, MS Feld. Approach to single-molecule detection using surface enhanced resonance Raman scattering (SERRS) A Study using Rhodamine 6G on colloidal silver. Appl Spectrosc 46 780-784, 1995. [Pg.736]

Modern developments in equipment for the detection of Raman scattering make the method much more applicable to use in the area of forensic science. This chapter primarily discusses the new technique of surface-enhanced resonance Raman scattering (SERRS) which has potential not yet exploited in the forensic science area. However, before commencing a discussion of this technique, a brief review of the use of Raman scattering in forensic science is given. [Pg.741]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...