SERRS scattering:surface-enhanced

Surface-enhanced resonance Raman scattering (SERRS), 21 327-328 advantage of, 21 329 Surface Evolver software, 12 11 Surface excess, 24 135, 136 Surface extended X-ray absorption fine structure (SEXAFS), 19 179 24 72 Surface filtration, 11 322-323 Surface finish(es). See also Electroplating in electrochemical machining, 9 591 fatigue performance and, 13 486-487 Surface finishing agents, 12 33 Surface force apparatus, 1 517 Surface force-pore flow (SFPF) model,... 

Another method for assaying the activity and stereoselectivity of enzymes at in vitro concentrations is based on surface-enhanced resonance Raman scattering (SERRS) using silver nanoparticles (116). Turnover of a substrate leads to the release of a surface targeting dye, which is detected by SERRS. In a model study, lipase-catalyzed kinetic resolution of a dye-labeled chiral ester was investigated. It is currently unclear how precise the method is when identifying mutants which lead to E values higher than 10. The assay appears to be well suited as a pre-test for activity. 

Li et al. have performed a comparative study on the surface-enhanced resonance hyper-Raman scattering and surface-enhanced resonance Raman scattering (SERRS) of dyes adsorbed on Ag electrode and Ag colloid [210]. 

S S CONTENTS Preface, C. Allen Bush. Methods in Macromo-lecular Crystallography, Andrew J. Howard and Thomas L. Poulos. Circular Dichroism and Conformation of Unordered Polypeptides, Robert W. Woody. Luminescence Studies with Horse Liver Dehydrogenase Information on the Structure, Dynamics, Transitions and Interactions of this Enzyme, Maurice R. Eftink. Surface-Enhanced Resonance Raman Scattering (SERRS) Spectroscopy A Probe of Biomolecular Structure and Bonding at Surfaces, Therese M. Cotton, Jae-Ho Kim and Randall E. Holt. Three-Dimensional Conformations of Complex Carbohydrates, C. Allen Bush and Perse-veranda Cagas. Index. 

Surface-enhanced resonance Raman scattering (SERRS) has also been achieved using silver colloid aggregates produced in situ in the chip. This method was used to detect an azo dye, 5-(2,-methyl-3,5,-dinitrophenylazo)quinolin-8-ol, which is a derivative of the explosive, TNT. With this method, it was possible to detect 10 iL of 10 9 M dye (or 10 fmol). This represented a 20-fold increase in sensitivity over that achieved using a macro flow cell [739]. 

SERRS surface-enhanced resonance Raman scattering... 

Further enhancement can be observed if the exciting light additionally couples into an electronic transition of the adsorbate (surface-enhanced resonance Raman scattering, SERRS). In this case, enhancement factors of... 

The classical Raman effect produces only very weak signals. There are two techniques which very successfully enhance this effect. The resonance Raman spectroscopy RRS is making use of the excitation of molecules in a spectral range of electronic absorption. The surface-enhanced Raman spectroscopy SERS employs the influence of small metal particles on the elementary process of Raman scattering. These two techniques may even be combined surface-enhanced resonance Raman effect SERRS. Such spectra are recorded with the same spectrometers as classical Raman spectra, although different conditions of the excitation and special sample techniques are used (Sec. 6.1). 

Surface enhanced resonance Raman scattering (SERRS) is an analytical technique with several advantages over competitive techniques in terms of improved sensitivity and selectivity. We have made great progress in the development of SERRS as a quantitative analytical method, in particular for the detection of DNA. However, one of the main advantages over fluorescence and other optical detection techniques is the ability to multiplex. 

Surface enhanced resonance Raman scattering (SERRS) [1-3] is a highly sensitive technique, so sensitive in fact that single molecule detection has previously been reported [4, 5]. It is a very attractive technique for the detection of biomolecules because it produces molecularly specific spectra which make it feasible to easily identify the components of a mixture in a single analysis without extensive separation procedures [6]. 

Faulds K, Fruk L, Robson DC, Thompson DG, Enright A, Smith WE, Graham D (2006) A new approach for DNA detection by SERRS. Faraday Discuss 132 261-268 Faulds K, Smith WE, Graham D (2004) Evaluation of surface enhanced resonance Raman scattering (SERRS) for highly sensitive and quantitative DNA analysis. Anal Chem 76 412-417... 

Graham D, Mallinder BJ, Whitcombe D, Smith WE (2001) Surface enhanced resonance Raman scattering (SERRS) - a first example of its use in multiplex genotyping. ChemPhysChem 2(12) 746-748... 

A further resonant contribution to the enhancement is possible when the molecule has an electronic transition in resonance, or dose to resonance, with the exciting laser so that one obtains surface-enhanced resonant Raman scattering or surface-enhanced resonant Raman spectroscopy (SERRS). In these circumstances the resonant enhancement typically contributes an additional factor of between 100 and 1000 to the intensity of the signal and this makes SERRS a particularly attractive approach for analytical apphcations because of its extremely high sensitivity. 

Faulds, K., Jarvis, R., Smith, W.E., Graham, D., and Goodacre, R. (2008) Multiplexed detection of six labelled oligonucleotides using surface enhanced resonance Raman scattering (SERRS). Analyst, 133, 1505-1512. 

Using biomolecules with chromophoric groups the Raman bands are both resonance (RRS) — and surface enhanced (RRS + SERS = SERRS). Instead of the usual term SERS, the Raman effect is, in this case, called surface enhanced resonance Raman scattering (SERRS). SERRS spectroscopy was first applied to biochemistry of heme chromophores by Cotton et al. Since then, SERS and SERRS have been extended to systematic investigations of biomolecules in the adsorbed state... 

Surface Enhanced Resonance Raman Scattering (SERRS)... 

Surface Enhanced Raman Scattering of Biomolecules Table 5. Resonance Raman spectroscopy of biological chromophores (SERRS studies are underlined)... 

K. Kneipp, Y. Wang, R. R. Dasari, and M.S. Feld, Approach to Sin -Molecute Detection Using Surface-Enhanced Resonance Raman-Scattering (SERRS) - a Study Using Rhodamine 6g on Colloidal Silver, Appl. Spectrosc. 49, 780 (1995)... 

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