Multiplexed detection

Nelson, R. W. Jarvik, J. W. Taillon, B. E. Tubbs, K. A. BIA/MS of epitope-tagged peptides directly from E. coli lysate Multiplex detection and protein identificatin at low-femtomole to subfemtomole levels. Anal. Chem. 1999, 71, 2858-2865. 

Wilson R, Cossins AR, Spiller DG (2006) Encoded microcarriers for high-throughput multiplexed detection. Angew Chem Int Ed Engl 45 6104—17... 

ML Marras, S. A., Kramer, F. R., et al. Multiplex detection of single-nucleotide variations using molecular beacons. Genet. Anal. 14(5—6), 151—156 (1999). 

Lee, H. J., Nedelkov, D. and Corn, R. M. (2006) SPR imaging measurements of antibody arrays for the multiplexed detection of low molecular weight protein biomarkers. Anal. Chem. 78, 6504-6510. 

Radioactivity, chemiluminescence or fluorescence have traditionally been used to monitor signals with protein arrays. The use of fluorescent dyes allows for increased sensitivity of detection, the ability to multiplex detection of several targets, and easy interfacing with detection instrumentation and automation. Surface plasmon resonance spectroscopy is another alternative, but successful development and commercialization has not yet been achieved. 

In the following sections, different methods of establishing functional DNA-protein conjugates are discussed, following a short historic overview of IPCR method development. As IPCR offers additional advantages in addition to the obvious enhancement of sensitivity, the unique potential for multiplex detection and an increased linear quantification range of the method is introduced. We show how various ELISA types suitable for... 

Furthermore, a simultaneous multiplex detection of several antibodies is impossible with the sequential incubation of biotinylated compounds and STV, as there is no selective linkage between a specific antibody and an individual DNA marker. 

Gerry NP, Witowski NE, Day J, Hammer RP, Barany G, Barany F. Universal DNA microarray method for multiplex detection of low abundance point mutations. J Mol Biol 1999 292 251-262. 

Protocol V Multiplex detection of mutations in clinical isolates of Rifr... 

Li HA, Cao ZJ, Zhang YH, Lau CW, Lu JZ (2010) Combination of quantum dot fluorescence with enzyme chemiluminescence for multiplexed detection of lung cancer biomarkers. Anal Meth 2 1236-1242... 

Keywords Total internal reflection Fluorescence Biosensor Chemical sensor Multiplex detection Array... 

Ngundi MM, Shriver-Lake LC, Moore MH et al (2006) Multiplexed detection of mycotoxins in foods with a regenerable array. J Food Prot 69 3047-3051... 

Because of the importance of micro-RNAs in gene regulation in both plants and animals, there is a need for profiling their expression in biological samples (51). Multiplexed detection methods in solution and in solid phase have been developed (52). 

Wark AW, Fee HJ, Corn RM. Multiplexed detection methods for profiling microRNA expression in biological samples. Angew. Chem. Int. Ed. 2008 47 644-652. 

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