Sulphated glycoprotein

Another metabolite isolated from the specie, Pseudopterogorgia americana, is corallan, 39, the chemistry of which was investigated by Molchanova and coworkers [29], Corallan, 39, a sulphated glycoprotein contains D-glucose, D- and L-galactoses, D-arabinose, L-flicose and D-glucoronic acid. Some structural features of the polysaccharide moiety of 39 are shown in scheme 3. 

Entactin Sulphated glycoprotein, dumbell shaped, 150 kDa single chain Extracellular matrix Binds laminin ... 

A homogeneous sulphated glycoprotein composed of protein (40%), carbohydrate (53%), and sulphate (1.6%) and containing D-galactose, 2-amino-2-deoxy-D-galactose, 2-amino-2-deoxy-D-glucose, and neuraminic acid as the principal sugars, has been isolated from the small intestine of the rabbit. 

Sulphated glycoproteins (mol. wts. >2.4 x 10 ) are biosynthesized, at least in the uterine luminal epithelial cells of the rat, under the stimulation of oestrogen but not progesterone, which inhibits their biosynthesis. ... 

Sulphated glycoproteins from porcine fundic mucosa inhibit pepsin activity by becoming bound to proteins. The physiological role of these sulphated glycoproteins is speculated to be in the protection of the components of the gastric gland from peptic digestion. 

Hormonal effects on the biosynthesis of sulphated glycoprotein in the endometrium of rabbit uterus have been shown to be confined to the microsomal level. Oestrogen increases the incorporation of L-fucose and 2-acetamido-2-deoxy-D-glucose from their respective nucleotides into a microsomal fraction, while progesterone suppresses the incorporation of the two sugars. 

Pathological Renal calculi Ca salts sulphate Glycoproteins... 

Extraction with urea of the insoluble material obtained following the digestion of rabbit uterus with collagenase yielded a structural glycoprotein (mol. wt. 1.6 X 10 ) containing 5.1% of carbohydrate. A sulphated glycoprotein has been obtained from endometrial scrapings of rabbit uterus after treatment with... 

Glycoproteins or mucoproieins. Compounds of proteins with carbohydrates. All glycoproteins contain a hexosamine and usually sulphate, ethanoate and glucuronic acid. The carbohydrate-protein linkages are, in some cases covalent and in others of the salt type. Aqueous solutions of glycoproteins are extremely viscous. 

Herring, G. M. Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate. Calc. Tiss. Res. 24, 29 (1977)... 

The main constituents of the extracellular matrix are (1.) collagen, particularly the fibrillary types I (36%), III (36%) and V (16%) as well as the non-fibrillary types FV (9%), VI (0.2%) and to a minor degree also type VII, (2.) glycoproteins (fibronectin, tenascin, laminin, undulin, elastin, entactin), and (2.) proteoglycans of both the core protein and the glycosaminoglycan type (e.g. chondroitin, heparan sulphate, hyaluronic add, dermatan sulphate). 

These proteins are composed of simple proteins in combination with carbohydrates like mucopolysaccharides, which include hyaluronic acid and chondroitin sulphates. On hydrolysis, mucopolysaccharides yield more than 4% of amino-sugars, hexosamine and uronic acid e.g., ovomucoid from egg white. Soluble mucoproteins are neither readily denatured by heat nor easily precipitated by common protein precipitants like trichloroacetic acid or picric acid. The term glycoproteins is restricted to those proteins that contain small amounts of carbohydrate usually less than 4% hexosamine. 

Like a number of polyanionic compounds, including sulphated polysaccharides, polyhydroxycarboxylates and various tannins, the flavonoids that we tested seem to interact with the surface glycoprotein gpl20 to prevent binding of the virus to the sCD4 receptor (41). 

Sulphated PS, potent antiviral agents, were also evaluated in vitro as inhibitors of influenza virus replication [119], The fact that the sulphated PS are inhibitory to some myxoviruses and retroviruses but not to others seems to depend on the composition of the amino acid sequences of the viral envelope glycoproteins that are involved in virus-cell binding and fusion [120],... 

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