Collagenases bacterial

BACKGROUND Structure and function of collagens Bacterial and mammalian collagenases Bacterial collagenases... 

In vitro degradation with collagenase. Bacterial collagenase from... 

MA M9 M09.001 Bacterial collagenase (Vibrio-type) Potential agent for burn debridment... 

The PL-catechin conjugate showed greatly amplified concentration-dependent inhibition activity against bacterial collagenase (ChC) on the basis of the catechin unit, which is considered to be due to effective multivalent interaction between ChC and the catechin unit in the conjugate. The kinetic study suggests that this conjugate is a mixed-type inhibitor for ChC. Hyaluronidase is an enzyme which catalyzes hydrolysis of hyaluronic acid and is often involved in a number... 

Synthetic Inhibitors of Bacterial and Mammalian Interstitial Collagenases... 

Figure 8.1. Schematic showing the hydrolysis of the type I tropocollagen (TC) heterotrimer [ol1(I)]20t2(I), by tissue (right) and bacterial (left) collagenases.

A number of other scissile bond replacements have been explored in an attempt to produce inhibitors of the bacterial collagenases, but all have exhibited relatively low potency Table 8.6). These include a compound with an A-methylamide residue in subsite P l (46), two carboxylates (47)-(48), the hydroxamate N-terminal (49) and C-terminal analogues (50)-(51), and one A-carboxyalkyl (52) derivative. 

Bacterial and mammalian collagenases their inhibition, 29(1992) 271 1-Benzazepines, medicinal chemistry of, 27... 

In a parallel preliminary experiment, hypochlorite instead of colla-genase was employed for matrix degradation. After hypochlorite treatment, tubules in sound dentin beneath the lesions contained material positive for PAS- and Richardson s stains, which was not observed after the buffer- and collagenase treatments. The bacterial degradation of the organic matrix was, therefore, simulated enzymatically. 

Lesion in bovine dentin with tubules protruding from degraded intertubular matrix (left degraded matrix right intact matrix). Demineralization in 0.1 M acetic acid pH 4.0, with subsequent exposure to bacterial collagenase. Fixed and demineralized with glutar-dialdehyde-acetic acid, post-fixed with osmium tetroxide ultrathin sections stained with uranyl acetate - lead citrate. 

Herring, G. M. Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate. Calc. Tiss. Res. 24, 29 (1977)... 

Other well-known zinc proteases are collagenase angiotensin-converting enzyme (important in regulating blood pressure) thermolysin, a bacterial endopep-tidase of Mr 34 600 containing 316 residues in its single polypeptide chain 144 and the Zn2+ G protease from Streptomyces albus, a D-alanyl-D-alanine car-boxypeptidase that catalyzes. carboxypeptidation and transpeptidation in cell wall metabolism. 

Oshima, G., Shimabukuro, H., and Nagasawa, K. 1979. Peptide inhibitors of angiotensin I-converting enzyme in digests of gelatin by bacterial collagenase. Biochim. Biophys. Acta 566, 128-137. 

---