Soybean cells

Koch, G., Wells, R. Grisebach, H. (1987). Differential induction of enzyme in soybean cell cultures by elictor or osmotic stress. Planta, 171, 519-24. 

R. M. Zacharius and E. B. Ralan, Isoflavonoid changes in soybean cell suspensions when challenged with intact bacteria of fungal elicitors. J. Plant Physiol 135 122... 

Hepatitis B surface antigen (HBsAg) Soybean cell culture No immunogenicity assays performed. - 95... 

Plant. Fewer and Owen (1989) found 3,5,6-trichloro-2-pyridinol as the major metabolite in plants. Cultured soybean cells metabolized triclopyr to dimethyl triclopyr-aspartate and dimethyl triclopyrglutamate which can be rehydrolyzed to form the parent compound. 

Fewer, P. and Owen, WJ. Amino acid conjugation of triclopyr by soybean cell suspension cultures. Pestle. Biochem. Physiol, 33 249-256, 1989. 

Wang et al. (1996) found that a 1 ppm solution of 1,4-dichlorobenzene was taken up by carrots Daucus car Ota, 49%), soybeans Glycine max, 50%), and red goosefoot Chenopodium rubrum, 62%), but not by tomatoes (Lycopersicon esculentum). Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenol. The authors further observed that the uptake, metabolism, and toxicity of 1,4-dichlorobenzene differed among the species tested. 

Welle, R. and Grisebach, H., Phytoalexin synthesis in soybean cells elicitor induction of reductase involved in biosynthesis of 6 -deoxychalcone. Arch. Biochem. Biophys., 272, 97, 1989. 

Terzaghi, W.B., Bertekap, R.L., and Cashmore, A.R., Intracellular localization of GBF proteins and blue light-induced import of GBF2 fusion proteins into the nucleus of cultured arahidopsis and soybean cells. Plant /., 11, 967, 1997. 

Plant cell culture has been used extensively for the production of biopharmaceuticals. A few examples are illustrated here, and a more extensive list can be found in Table 6.4. Smith et al. (2002) employed both soybean and tobacco cell lines to produce a hepatitis virus surface antigen (HBsAg), to be used as a vaccine, in shaker flask cultures. The authors found that the titers of HBsAg in soybean cell culture were 65 pg/g fresh weight, and 10-fold lower in tobacco cell culture, resulting in productivities of 1 mg/L/d and 0.16 mg/L/d, respectively. These numbers correspond well with those found for yeast batch cultures (1.5 mg/L/d). 

Averyhart-Fullard, V., Datta, K. Marcus, A. (1988). A hydroxy-proline-rich protein in the soybean cell wall. Proceedings of the National Academy of Sciences (USA) 85, 1082-5. 

A feature of some pterocarpan phytoalexins (e.g., pisatin and glyceollin of pea and soybean, respectively) is their hydroxylation at position 6a, a reaction catalyzed by a microsomal cytochrome P450 monooxygenase.49 50 A cDNA encoding this enzyme was recently characterized from elicited soybean cell cultures.51 The microsomal protein, expressed in yeast cells, catalyzed the stereoselective hydroxylation of (6a/ , lla/ )-3,9-dihydroxypterocarpan to its 6a-hydroxy derivative. It was also demonstrated that the enzyme expression is regulated at the transcriptional level.51... 

HAGMANN, M.-L., HELLER, W., GRISEBACH, H Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures. Eur. J.Biochem., 1984,142,127-131. 

Additions of valine, leucine, and isoleucine were found to relieve the growth inhibitory effects of TP on Bacillus cell cultures, soybean cell cultures, and Arabidopsis seedlings. ALS isolated from a number of sources was found to be sensitive to TP at nM levels. The barley enzyme has been amenable to purification. A purification procedure that gives >60 % recovery and 235-fold purification is described. 

Contents P.LKing Plant Tissue Culture and the Cell Cycle. - KHahlbrock, J. Schroder, J. Vieregge Enzyme Regulation in Parsley and Soybean Cell Cultures. - P. J. Wang,... 

Lee, S., Park, J. and Lee, Y., 2003, Phosphatidic acid induces actin polymerization by activating protein kinases in soybean cells. Mol. Cell 15 313-319. 

Shigaki, T. and Bhattacharyya, M.A., 2000, Decreased inositol 1,4,5-trisphosphate content in pathogen-challenged soybean cells. Mol. Plant-Microbe Interactions 13 563-567. 

A GST enzyme from pea Is very effective In catalyzing GSH conjugation of the herbicide fluorodlfen ( ). This enzyme has a pH optimum and other properties that are comparable to mammalian GST enzymes (85). This enzyme activity was observed In other plant species, but fluorodlfen resistant species appeared to have higher levels of this enzyme than susceptible species ( ). Additional studies with pea Indicated the presence of two soluble GST Isozymes, one that utilized fluorodlfen and one that utilized -cinnamic acid as substrates (W7). These Isozymes appeared to form aggregates during purification. In addition, a microsomal GST was detected In pea that utilized both -cinnamic acid and benzo(a)pyrene as substrates (WT.). Soybean cell suspension cultures metabolized -clnnamlc acid In a 6% yield to a product that corresponded to the GSH conjugate of t-clnnamlc acid by paper chromatography (107). 

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