Soybean cells Subject

Figure 3. Distribution of C in plant tissues as a function of solubility. Barley, corn, cotton, peanut cell cultures, and soybeans were treated with V C] PCNB for 3 days. Lake water rich in blue green algae was treated for 9 h. Peanut plants were treated for 2 days and subjected to a 2-day post-treatment incubation.

G proteins are located in different compartments within the cell (Nurnberg and Ahnert-Hilger, 1996). Although most G proteins are found attached to the plasma membrane and intracellular membranes, some are also located within the cytoplasm (Rudolph et al., 1989). Therefore, G proteins in preparations of disrupted cells, or in cell and tissue extracts are also subject to pertussis toxin-mediated ADP-ribosylation. In this case, precautions have to be taken to prevent proteolysis, and protease inhibitors should be included in the buffer (aprotonin, p-aminobenzamidine, leupeptin, phenylmethylsul-fonyl fluoride, or soybean or lima bean trypsin inhibitors) (Carty, 1994). 

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