Sodium butyrate effect

This study describes the effect of sodium butyrate on glyco-lipids from four human colonic tumor cell lines, SKCO-1, HT-29, SW-480 and SW-620 and a human fetal intestinal line, FHS. 

Effect of Sodium Butyrate on Morphology and Cell Growth. FHS, SKCO-1, HT-29 did not show any significant morphological changes with sodium butyrate. SW-480 and SW-620 cells produce angular cells rich in cellular membranes. These processes were pronounced with SW-620 cell lines. 

In the present study, sodium butyrate had a differentiated effect on cell morphology. Sodium butyrate caused the SW-620 lines to become markedly angular with extension of many membraneous processes. These effects were also seen with the SW-480 cell lines but were less pronounced. No morphological changes were observed when SKCO-1, HT-29 and FHS cell lines were cultured in sodium butyrate. 

The concentration of sodium butyrate was observed to have a differential effect on cell growth in colonic cell lines. After culturing for 8 days with 5 mM sodium butyrate, the cell protein per flask of the SCKO-1 line was decreased to less than 10% of the control cultures. In the SW-620 culture,cell protein per... 

Table IV. Effect of Sodium Butyrate on Choleragen Receptors and GM1 Content of HeLa Cells...

Kruh, J., Effects of sodium butyrate, a new pharmacological agent, on cells in culture, Mol. Cell. Biochem., 42, 65-82, 1982. 

Yanagi, S., Yamashita, M., Imai, S. 1993. Sodium butyrate inhibits the enhancing effect of high fat diet on mammary tumorigenesis. Oncology. 50, 201-204. 

Several other pyrolytic studies were performed on nylon 6. In one such study [4], the influence of several aliphatic carboxylates on nylon 6 thermal degradation was studied. The carboxylates that were evaluated include sodium butyrate, sodium caproate, sodium a-ethylcaproate, sodium caprylate, sodium laurate, potassium caproate, potassium laurate, and lithium caproate. Small amounts of these aliphatic carboxylates strongly increase the thermal decomposition rate even at 280° C. The effect of aliphatic carboxylates can be explained by the deprotonation of one of the amide groups of the polymer followed by the nucleophilic substitution of a neighboring carbonyl group, in a reaction as shown below ... 

Baker PN, Morser J Burke DC (1980) Effects of sodium butyrate on a human lymphoblastoid cell line (Namalwa) and its interferon production. Journal of Interferon Research 1 (1) 71-77. 

Gum LR, Kam WK, Byrd LC et al (1987) Effects of sodium butyrate on human colonic adenocarcinoma cells. Induction of placental-Uke alkaline phosphatase. L Biol Chem 262 1092-1097... 

HDAC inhibitors were found to increase both the expression of SMN2 and Smn protein in various cell types. The initial evidence regarding their potential therapeutic utility in SMA came from the studies of a weak, non-specific HDAC inhibitor phenylbutyrate [47]. Both this agent along and sodium butyrate showed promise in a mouse model and in an open-label pilot study, but was not effective in a human Phase II clinical trial [40, 48, 49]. 

Susan Perrtne of the Children s Hospital Oakland Research Center decided to try to reawaken the dormant fetal globin gene. She and her colleagues injected a sodium butyrate solution (the sodium salt of butyric acid) into three sickle cell patients and three (3-thalassemia patients. As a result of the two- to three-week treatment, fetal hemoglobin production was boosted as much as 45% in these individuals. One (3-thalassemia patient even experienced a complete reversal of the symptoms. Moreover, this treatment had few adverse side effects. 

C.B. Lozzio, B. B. Lozzio, E. A. Machado, J. E. Fuhr, S. V. Lair, and E. G. Bamberger, Effects of sodium butyrate on human chronic myelogenous leukaemia cell line K562, Nature, 281 (1979) 709-710. 

Owen, M.A.G., Waines, R, Bradley, G. and Davies, S. (2006) The effect of dietary supplementation of sodium butyrate on the growth and microflora of Clarias gariepinus (Burchell 1822). Proceedings of the XII International Symposium Fish Nutrition Feeding, May 28 - June 1, 2006 149. 

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