Smooth muscle cells, isolated

Wisniewski HM, Frackowiak J, Mazur Kolecka B. In vitro production of beta-amyloid in smooth muscle cells isolated from amylid angiopathy-affected vessels. Neurosci Lett 1995 183 120-123. 

Shmigol A, Eisner DA, Wray S 1998b Carboxyeosin decreases the rate of decay of the [Ca2+]j transient in uterine smooth muscle cells isolated from pregnant rats. Pfitigers Arch 437 158-160... 

Wang Q, Hogg RC, Large WA 1992 Properties of spontaneous inward currents recorded in smooth muscle cells isolated from the rabbit portal vein. J Physiol 451 525—537 ZhuGe R, Sims SM, Tuft RA, Fogarty KE, Walsh JV 1998 Ca2+ sparks activate K+ and Cl-channels, resulting in spontaneous transient currents in guinea-pig tracheal myocytes. J Physiol 513 711-718... 

Airway smooth muscle cells isolated from canine tracheae and bronchi subjected to cyclic strain exhibit increased cell number and DNA synthesis in cell culture. The content of total cellular protein, especially contractile proteins including myosin, myosin light chain kinase, and desmin, was increased compared to cells cultured under static conditions. 

Costa P, Soulie-Vassal ML, Sarrazin B, Rebillard X, Navratil H, Bali JP. Adrenergic receptors on smooth muscle cells isolated from human penile corpus cav-ernosum. J Urol 1993 150 859-863. 

Aaronson, P.I. and Benham, C.D. (1989) Alterations in [Ca ]i mediated by sodium-calcium exchange in smooth muscle cells isolated from the guinea-pig ureter. Journal of Physiology, 4Iftl-18. 

Fukumitsu, T., Hayashi, H., Tokuno, H., and Tomita, T. (1990) Increase in calcium channel current by -adrenoceptor agonists in single smooth muscle cells isolated from porcine coronary artery. British Journal of Pharmacology, 100 593-599. 

Hughes, A.D., Parkinson, N.A., and Wijetunge, S. (1996) alpha2-Adrenoceptor activation increases calcium channel currents in single vascular smooth muscle cells isolated from human omental resistance arteries. Journal of Vascular Research, 33 25-31. 

Loirand, G., Pacaud, P., Baron, A., Mironneau, C., and Mironneau, J. (1991) Large conductance calcium-activated non-selective cation channel in smooth muscle cells isolated from rat portal vein. Journal of Physiology (London), 437 461-475. 

Worley, J.F., Deitmer, J.W., and Nelson, M.T. (1986) Single nisoldipine-sensitive calcium channels in smooth muscle cells isolated from rabbit mesenteric artery. Proceedings of the National Academy of Sciences of the United States of America, 83 5746-5750. 

Orlandi A, Ehrlich P, Ropraz P, Spagnoli LG, Gabbiani G (1994) Rat aortic smooth muscle cells isolated from different layers and at different times after endothelial denudation show distinct biological features in vitro. Arterioscler Thromb 14 982-989... 

ROCK pathway. In a subsequent study by Haydont et al. (2005), Pravastatin, a hydrophiUc statin, reduced CTGF, TGF(3, and collagen production from intestinal smooth muscle cells isolated from patients with radiation enteritis and improved radiation enteropathy in an animal model (haydont et al. 2007a). 

Inoue Y, Sperelakis N 1991 Gestational change in Na+ and Ca2+ channel current densities in rat myometrial smooth muscle cells. Am J Physiol 260 C658-C663 Kamishima T, McCarron JG 1996 Depolarization-evoked increases in cytosolic calcium concentration in isolated smooth muscle cells in rat portal vein. J Physiol 492 61-74 Khan R, Mathroo-Ball B, Arulkumaran S, Ashford ML 2001 Potassium channels in the human myometrium. Exp Physiol 86 255-264... 

Ca2+ signals viewed in isolated smooth muscle cells... 

FIG. 2. Confocal image of an isolated smooth muscle cell from guinea-pig ileum which has been permeabilized with staphylococcal os-toxin and incubated with 150 /iM Fluo-3 acid which stains the SR. The SR is predominantly localized to the periphery of this type of smooth muscle as seen on the left hand side where the image plane is through the centre of the cell, whereas an extensive network is seen where the image plane is adjacent to the plasma membrane as seen in the right hand portion of the cell. 

The fermentation broth of an unidentified endophytic fungus isolated from plants growing in Hawaii has afforded microcarpalide I (59), an alkyl-substituted nonenolide that is weakly cytotoxic as a result of its ability to disrupt microfilaments. Extracts from the strain 112/13 collected from the bark oiFicus microcarpa L. (Moraceae) demonstrated a strong abrogation of microfilament activity. In A-10 rat smooth muscle cells, a 5 Xg/mL dose was able to induce a 50%—75% loss of actin filaments. Microcarpalide 1... 

Resident CSCs were first isolated in murine hearts. Characterization of these cells was based on the expression of the stem cell-related surface antigens c-Kit and Sea-1. In the first study, freshly isolated c-Kit /Lin cells were shown to be clono-genic and to differentiate into myocytes, smooth muscle cells, and endothelial lineage cells [35]. Those cells generated functional myocardium when injected into ischemic areas of the heart. The second study characterized CSCs as Sca-l/c-Kit. When treated in culture with 5-azacytidine, those cells differentiated into a myogenic lineage. Subsequently, intravenous injection of the cells in an ischemia/reperfusion model resulted in infarct healing with cardiomyocyte transdifferentiation... 

Cardiovascular heart diseases (CHD) are considered as the clinical expression of advanced atherosclerosis. One of the initial steps in atherogenesis is the oxidative modification of LDL and the uptake of the modified lipoprotein particles by macrophages, which in turn become lipid laden cholesterol-rich cells, so-called foam cells [159]. An accumulation of foam cells in the arterial wall is the first visible sign of atherosclerosis and is termed fatty streak, the precursor to the development of the occlusive plaque [160]. It is well known that oxidation of LDL can be initiated in vitro by incubating isolated LDL particles with cells (macrophages, lymphocytes, smooth muscle cells, or endothelial cells), metal ions (copper or iron), enzymes, oxygen radicals, or UV-light. However less is known about the mechanisms by which... 

Boyer, J.-C., Christen, M.-O., Balmes, J.-L., Bali, J.-P., Bianchi, B. Calcitonin gene-related peptide-induced relaxation of isolated human colonic smooth muscle cells through different intracellular pathways, Biochem. Pharmacol. 1998, 56, 1097-1104. 

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