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Preparation of Slides

05% Coomassie Brilliant Blue G250 (w/v) or 0.1% Amido Black 10 B in B PBS [Pg.152]

Melt Reagent A in a water bath or in a microwave oven. Dilute some milliliters of the molten solution with hot water to get 0.5% agar or agarose. Pour this solution onto a carefully defatted slide (3x7 cm) and dry in a stream of warm air. Store a stock of these pre-coated slides dusdess they are stable at RT for months. [Pg.152]

Pipet 4 ml of the hot molten Soln. A onto the covered slides laying on a horizontally leveled table. After gelation, punch wells using a stamp as shown in Fig. 4.1. Suck out carefully residual material from the wells using a Pasteur pipette connected to a pump. [Pg.152]


Preparation of Slides Using CaSki (or Other Adherent Cultured) Cells... [Pg.391]

Preparation of Slides—Most material may be dispersed easily in xylene or balsam. When these liquids dry the material remains firmly adherent to the glass slide. Dunn (1930) recommends the use of a stiff brush when mounting small particles with xylene or balsam. He claims the use of a brush in smearing the slide tends to produce a uniform distribution. Cover glasses may be used on the slide, but it must be remembered that under high magnification the use of a cover glass interferes... [Pg.90]

Observations on the preparation of slides for particle-size determination. J. Ind. flyg. Toxicol., 24 51-52. [Pg.530]

The need for deparaffinization of FFPE slides for FISH analysis does not differ from standard preparation of slides for histological staining methods like H E, special stains or IHC. Following mounting by baking, the slides are deparaffinized in xylene (or substitutes) and rehydrated in a series of ethanol. [Pg.67]

The animated and interactive blackboard easy access to attractive and realistic graphics (graphs, preparation of slides, animated molecular models, statistics packages, spreadsheets) possibility of interaction. [Pg.324]

Routine microscopic counts, like all bacterial counts, are to be regarded as estimates of numbers only. They cannot be made with absolute accuracy even with the most careful technique. Errors will arise from inaccuracies in measurement of the minute quantities of milk examined at any one time, from faulty staining or preparation of slides, from mistakes in observation and the like. These limitations, while important, are not difficult to overcome in sufficient measure to make microscopic grading a satisfactory method of controlling the quality of unpasteurized milk. As it is only in this way that counts of the bacteria themselves can be made, it must be recognized that accurately carried out microscopic counts of individual bacteria give the truest picture of the actual conditions of raw milk that can be obtained with any technique. [Pg.379]

Preparation of Slides. A 2.5 x 2 cm. slide, about 0.5 mm. thick, is sawed and groimd from a sound and homogeneous part of the stone being studied. It is then polished with finer and finer abrasives and the operation is finished with alumina obtained after 5 hours of sedimentation. [Pg.313]

Theisen, A. A. M. E. Harward, 1962. A paste method for preparation of slides for clay mineral identification by X-ray diffraction. Sod. Sci. Soc Amer. Proc. 26 90-91. [Pg.185]

Propylene oxide is an allergic and irritant agent, used as a solvent and raw material in the chemical industry as starting material and intermediate for a broad spectrum of polymers. It can be used as a dehydrating agent for the preparation of slides in electron microscopy. Occupational dermatitis was also reported while using a skin disinfectant swab. [Pg.1169]

Preparation of slides for mounting of vibratome sections Take a slide and create two narrow ridges by means of applying nail polish (Fig.l)... [Pg.59]


See other pages where Preparation of Slides is mentioned: [Pg.79]    [Pg.148]    [Pg.151]    [Pg.390]    [Pg.176]    [Pg.76]    [Pg.99]    [Pg.96]    [Pg.26]    [Pg.182]    [Pg.677]    [Pg.680]    [Pg.303]    [Pg.59]    [Pg.244]   


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Slide preparation

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