Microscopy counting slide preparation

Once the sample was prepared, meristems were scored with light microscopy using the x40 objective, and the mitotic index was estimated by counting up a total number of 1000 cells in three slides of the same sample. 

Microscope slide methods A specimen smear on a glass slide followed by simple or differential staining remains one of the fastest ways to determine numbers of bacteria or yeasts. A minimum number of cells of 10" cm are needed. Both viable and nonviable cells may be enumerated and results can be obtained in 5 min. A number of slide counting devices exist with the Breed slide method being one of the most widely used. The Breed smear was one of the first methods to use microscopy for counting bacteria in milk and involved the preparation of milk films, staining with Methylene Blue, and then microscopic examination. 

---