PREPARING MOLD SLIDE CULTURES

Prepare lactophenol—cotton blue dye by mixing equal volumes of component A and component B. 

Pour approximately 10mL of sterile molten potato dextrose agar into a Petri plate and allow to solidify. 

Prepare a sterile (autoclaved) growth chamber (glass Petri plate, sterilized tubing, sterile microscope slide and cover slip) as shown in Fig. 12.4. 

Using a sterile spatula, cut the agar into cubes of suitable size that fit within the dimensions of the microscope slide cover slip. 

Using a sterile needle, collect a portion of mycelial mass and aseptically inoculate the corners of the agar cuhe. 

---