Slide preparation living cells

Except for some of the fixation studies (for which Raman data were also acquired) and for studies in aqueous media (see later), all sample preparations used reflection-coated glass slides as sample substrates. Raman data are prone to show some glass signatures when these slides are used consequently, some studies that relied on a combination of Raman and IR data were carried out on CaF2 disks. Similarly, CaFj was used as a substrate for IR studies of live cells, as prolonged exposure to water damaged the reflective slides. 

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