Cytogenetic analysis

B. Cytogenetic analysis in cultured Chinese hamster or human cells... [Pg.290]

The major Impetus to the development of methods for the prenatal detection of genetic disorders derives. In historical terms, from the roughly simultaneous development of three major techniques (11-14). One was the technique, and the willingness to use It, for obtaining samples of amnlotlc fluid early In gestation. The second was the development of techniques for the culture of human cells in vitro, and the third was the development of better techniques for cytogenetic analysis. As will be described below, with the availability of these three techniques It became possible first to work out methods for the examination of fetal chromosomes, and then, by extension, to devise ways of determining other characteristics of the fetus. [Pg.71]

Cytogenetic Analysis and Sex Determination. Since amnlotlc fluid cells are fetal In origin, they have the chromosome complement of the fetus Itself Therefore, the chromosomes of the fetus can be examined by karyotyping the cultured amnlotlc fluid cells There are two principal reasons for examining fetal chromosomes One, the numerically less frequent reason. [Pg.78]

Hematopathology evaluation of biopsy specimen-morphologic inspection, immunohistochemistry for cell-surface antigens to characterize lymphoma cells, cytogenetic analysis... [Pg.1375]

Cytogenetic analysis Laboratory identification of chromosomes to detect mutational defects. [Pg.1564]

A2. Anastasi. J., Interphase cytogenetic analysis in the diagnosis and study of neoplastic disorders. Am. J. Clin. Pathol. 95 Suppl. 1, S22-S28 (1991). [Pg.34]

Hozier, J., Sawyer, J., Moore, M., Howard, B., and Clive, D., Cytogenetic analysis of the L5178Y/TK TK mouse lymphoma mutagenesis assay system, Mutat. Res., 84,169,1981. [Pg.310]

A positive result from a chromosome aberration assay (MNT, cytogenetic analysis or small colonies in MLA) suggests a second in vivo assay for clastogenesis or aneugenesis. Centromere staining of mitotic cells allows the distinction of aneugens and clastogens. [Pg.263]

Cytogenetic analysis of patients with chronic myelogenous leukemia (CML) reveals an unusual translocation between chromosomes 9 and22 termed the Philadelphia chromosome."... [Pg.212]

Thompson, E D. Hiles, R.A. (1981) A method for determining the maximum tolerated dose for in vivo cytogenetic analysis. Food Cosmet. Toxicol, 19, 347-351 Thompson, E.D., Coppinger, W.J., Piper, C.E., McCarroll, N., Oberly, T.J. Robinson, D. (1981) Mutageiucity of alkyl glycidyl ethers in three short-term assays. Mutat. Res., 90, 213-231... [Pg.485]

Kallioniemi A, Kallioniemi OP, Sudar D et al. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 1992 258 818-821. [Pg.86]

Ross DM, Branford S, Moore S et al. Limited clinical value of regular bone marrow cytogenetic analysis in imatinib-treated chronic phase CML patients monitored by RQ-PCR for BCR-ABL. Leukemia... [Pg.146]

Tice, R.R., Boucher, R., Luke, C.A. Shelby, M.D. (1987) Comparative cytogenetic analysis of bone marrow damage induced in male B6C3F1 mice by multiple exposures to gaseous 1,3-butadiene. Environ. Mutag., 9, 235-250... [Pg.222]

Fomenko, V.N. Katosova, L.D. (1973) The results of cytogenetic analysis of the peripheral blood in women workers in contact with chloroprene latex. In Kazan, Nauchnye Trudy Akusherkso-Ginekologich-eskaya Profpatologiya, pp. 33-37 (in Russian)... [Pg.247]

Sram, R.J., Zudova, Z. Kuleshov, N.P. (1980) Cytogenetic analysis of peripheral lymphocytes in workers occupationally exposed to epichlorohydrin. Mutat. Res., 70, 115-120... [Pg.627]

Pacchierotti, F., Bassani, B., Leopardi, P. Zijno, A.. (1991) Origin of aneuploidy in relation to disturbances of cell-cycle progression. 11 Cytogenetic analysis of various parameters in mouse bone marrow cells after colchicine or hydroquinone treatment. Mutagenesis, 6, 307-311... [Pg.717]

Pelclova, D., Rossner, P. Pickova, J. (1987) Cytogenetic analysis of peripheral lymphocytes in... [Pg.861]

Darroudi, F. Natarajan, A.T. (1983) Cytogenetic analysis of human peripheral blood lymphocytes in vitro treated with resorcinol. Mutat. Res.. 124. 179-189... [Pg.1128]

Oral Rat Mouse Intraperitoneal Rat Mouse Subcutaneous Rat Mouse Intravenous Mouse Dog Dermal Rabbit Cytogenetic analysis In vitro In vivo Ames test Chromosome Rat bone —S9 +S9 aberrations SCE marrow ... [Pg.115]

Klinger, K. (1995) Cytogenetic analysis, m Nonisotopic Methods in Molecular Biology A Practical Approach (Levy, E. R. and Herrington, C S, eds ), Oxford University Press, Oxford, UK, pp. 25—50... [Pg.395]

Anastasi, J, Thangavelu, M., Vardiman, J. W., Hooberman, A L., Bian, M L., Larson, R. A, and Le Beau, M M. (1991) Interphase cytogenetic analysis detects minimal residual disease m a case of acute lymphoblastic leukemia and resolves the question of origin of relapse after allogeneic transplantation. Blood 77, 1087-1091. [Pg.416]

Mostly negative results were obtained in animal studies following oral exposure to 2,3,7,8-TCDD. Cytogenetic analysis of the bone marrow did not reveal any increase in chromosomal aberrations in... [Pg.198]

Mammalian cells Cytogenetic damage CHO or other cell types In vitro cytogenetic analysis... [Pg.82]

Cytogenetic Mouse or rat bone marrow In vivo cytogenetic analysis... [Pg.82]

Cytogenetic damage Mouse (or other species) blood (lymphocytes) In vivo cytogenetic analysis Chromosomal aberrations <2 mo M H M M... [Pg.83]

Cytogenetic damage Mouse spermatocytes In vivo cytogenetic analysis Chromosomal rearrangements <3 mo M H S NA... [Pg.83]

Various types of cells can be scored for chromosomal aberrations. Spermatogonial cells, spermatocytes, oocytes, early embryos, bone marrow cells, and lymphocytes have been used successfully after in vivo treatments of an animal. For short-term cytogenetic analysis, however, an in vitro cell-culture assay is often chosen. Such in vitro assays usually use a peripheral lymphocyte system or a monolayer cell-culture system. [Pg.110]

Five plant systems or types of study have been selected for coverage in this report plant cytogenetic analysis, chlorophyll mutations, the Tradescantia stamen hair test, the maize waxy locus system, and plant metabolic activation systems. Descriptions of other plant systems may be found in the proceedings of workshops on the use of higher plants as monitors for environmental mutagens94 and on the use of pollen systems to detect biologic activity of environmental pollutants.71... [Pg.112]

CHROMATID GAP A small achromatic region of a (stained) chromatid, detected in cytogenetic analysis "gap" is used, rather than "break," if the achromatic region is smaller than the width of the chromatid. [Pg.238]

COLCEMID A chemical that inhibits the formation of the mitotic spindle that is involved in the division of eukaryotic cell nuclei colcemid (or the related substance colchicine) is used in some procedures to facilitate cytogenetic analysis. [Pg.239]

Schmid, W. The micronucleus test for cytogenetic analysis, pp. 31-53. In A. Hollaender, Ed. Chemical... [Pg.284]

Koshi K, Serita F, Sawatari K, et al. 1987. Cytogenetic analysis of bone marrow cells and peripheral blood lymphocytes from rats exposed to chromium fumes by inhalation. Mutat Res 181 365. [Pg.434]

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