Single-stranded conformational polymorphism

Progress in molecular biology has provided a new perspective. Techniques such as the polymerase chain reaction and single-strand conformation polymorphism analysis have greatly facilitated the molecular analysis of erythroenzymopathies. These studies have clarified the correlation between the functional and structural abnormalities of the variant enzymes. In general, the mutations that induce an alteration of substrate binding site and/or enzyme instability might result in markedly altered enzyme properties and severe clinical symptoms. 

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Single-strand conformation polymorphism (SSCP) analysis... 

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Single-strand conformation polymorphism (SSCP) Wastewater bioreactors (including denitrifying and phosphate-removal system, Chinese traditional medicine wastewater treatment system, beer wastewater treatment system, fermentative biohydrogen producing system, and sulfate-reduction system) Microbial community structures, diversity and distribution in different wastewater treatment processes, and relationship between the structures and the status of processes [157]... 

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Figure 6-5. Rapid and sensitive methods for detecting mutations in DNA. (a) Denaturing gradient gel electrophoresis. (b) Single-strand conformation polymorphism.

Several rapid and sensitive methods have been developed to detect mutations in cDNA and genomic DNA. These include ribonuclease (RNase) protection analysis, denaturing gradient gel electrophoresis, and single-strand conformation polymorphism analysis. These methods can be used in conjunction with the PCR technique to detect small deletions or insertions and single base substitutions. 

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