Products single-strand conformation polymorphism analysis

Suzuki Y, Orita M, Shiraishi M, Hayashi K, Sekiya T. Detection of ras gene mutations in human lung cancers by single-strand conformation polymorphism analysis of polymerase chain reaction products. Oncogene 1990 5 1037-43. 

Figure 12.1. Single-strand conformation polymorphism analysis. Single-stranded DNAs are generated by denaturation of the PCR products and separated on a nondenaturing polyacrylamide gel. A fragment with a single-base modification generally forms a different conformer and migrates difter-ently when compared with the wild-type DNA. Reproduced with permission Ifom Ref 9.

Duthoit F, Godon JJ, Montel MC (2003) Bacterial community dynamics during production of registered designation of origin Salers cheese as evaluated by 16S rRNA gene single-strand conformation polymorphism analysis. Appl Environ Microbiol 69 3840-3848... 

Hayashi, K. 1996. PCR-SSCP. Single strand conformation polymorphism analysis of PCR products. In Laboratory Protocols for Mutation Detection (U. Landegren, ed.), pp. 14-22. Oxford... 

Other methods that did not require sequence information but produced species-specific electrophoretic gel patterns were also developed. Single-strand conformational polymorphism analysis is one such method, which was used to discriminate fish and meat products. In this method, the same region of the DNA is amplified, double-stranded amplification products are denatured and single-stranded products are allowed to form their sequence-dependent secondary structures, and their corresponding migration patterns on the gel are observed [51]. 

DNA and RNA with different sequences and mutations show different electrophoretic migration behavior. There are simple electrophoretic methods, which can be used to detect mutations and minimal differences within the sequences of the PCR product. Popular methods are single-strand conformational polymorphism and heteroduplex analysis. 

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