Single-strand conformation polymorphism SSCP analysis

K11. Kanno, H Fujii, H., and Miwa, S Molecular heterogeneity of pyruvate kinase deficiency identified by single strand conformational polymorphism (SSCP) analysis. Blood 84 (Suppl. 1), 13a... 

Single-strand conformation polymorphism (SSCP) analysis... 

Teschauer, W., Mussack, T., Braun, A., Waldner, H. and Fink, E. (1996) Conditions for single strand conformation polymorphism (SSCP) analysis with broad... 

Jl. Jaksch, M., Gerbitz, K. D., and Kilger, C., Screening for mitochondrial DNA (Mt DMA) point mutations using non radioactive single strand conformation polymorphism (SSCP) analysis. Clin. Biochem. 28, 503-509 (1995). 

One of the most popular methods for identifying differences in the sequence of ampUcons is single-stranded conformation polymorphism (SSCP) analysis, which has been used very often in chnical genetic research (Hayashi, 1992 Bunge et al., 1996 Hestekin and Barron, 2006). In January 2007 more than 10,000 publications were listed in PubMedunder the term SSCP. In the last two decades, SSCP has also been found useful in tackling various problems related to food analysis. 

Rehbein H (2005). Identification of the fish species of raw or cold-smoked salmon and salmon caviar hy single-strand conformation polymorphism (SSCP) analysis. Eur. Food Res. Technol., 220 625-632. 

Fukuda, M. and Tamaki, Y., Subtyping of D11S488 STR alleles by single-strand conformation polymorphism (SSCP) analysis in two cases of disputed parentage, Nihon Hoigaku Zasshi, 52, 42, 1998. 

Mwenda JM, Flashiba K, Bambra CS, Shotake T. Analysis of primate major histocompatibility complex (MHC)-DQAl locus by PCR-single strand conformation polymorphism (SSCP). Cell Mol Biol 1997 43 471-475. 

Single-strand conformation polymorphism analysis (SSCP, SSCA) Scanning... 

Figure 37-i I A schematic of single-strand conformation polymorphism analysis (SSCP). PCR amplicons carrying the polymorphic site (shown as x and y) are diluted and then denatured to form single-stranded fragments. When the fragments are electrophoresed, they assume secondary structures. When conditions are right, polymorphisms are detected by differential band patterns compared with a known sample. 

Matrix-assisted laser desorption ionisation/time of flight (i.e., MALD-TOF) MS Electrospray ionization MS SSCP - Quantitative single-strand conformation polymorphism analysis... 

Rehbein H, Gonzales-Sotelo C, Perez-Martin R, et al. (1999b). Differentiation of sturgeon caviar by single strand conformation polymorphism (PCR-SSCP) analysis. Arch. Lebens-mittelhyg., 50 13-17. 

Rehbein H, Sotelo CG, Perez-Martin RI, et al. (2002). Differentiation of raw or processed eel by PCR-based techniques restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP). Eur. Food Res. Technol, 214 171-177. 

Teschauer W, Mussack T, Braun A, Waldner H, Fink E (1996). Conditions for single strand conformation polymorphism analysis with broad applicability a study on the effects of acrylamide, buffer and glycerol concentrations in SSCP analysis of exons of the p53 gene. Eur. J. Clin. Chem. Clin. Biochem, 34 125-131. 

Hayashi, K. 1996. PCR-SSCP. Single strand conformation polymorphism analysis of PCR products. In Laboratory Protocols for Mutation Detection (U. Landegren, ed.), pp. 14-22. Oxford... 

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