Electrophoresis single-strand conformation polymorphism

Hofman-Bang, J., et al.. High-efficiency multiplex capillary electrophoresis single strand conformation polymorphism (multi-CE-SSCP) mutation screening of SCN5A a rapid genetic approach to cardiac... 

Gasser, R.B., Zhu, X.Q. and Woods, W.G. (1999) Genotyping Taenia tapeworms by single-strand conformation polymorphism of mitochondrial DNA. Electrophoresis 20, 2834—2837. 

Stothard, J.R., Frame, IA. and Miles, MA. (1997) Use of polymerase chain reaction-based single strand conformational polymorphism and denaturing gradient gel electrophoresis methods for detection of sequence variation of ribosomal DNA of Trypanosoma cruzi. International Journal for Parasitology 27, 339-343. 

Snyder TM, McGown LB (2005) Multiplex single strand conformation polymorphism analysis by capillary electrophoresis with on-the-fly fluorescence lifetime detection. Appl Spectrosc 59 335-339... 

Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 1989 86 2766-2770. 

Figure 6-5. Rapid and sensitive methods for detecting mutations in DNA. (a) Denaturing gradient gel electrophoresis. (b) Single-strand conformation polymorphism.

Several rapid and sensitive methods have been developed to detect mutations in cDNA and genomic DNA. These include ribonuclease (RNase) protection analysis, denaturing gradient gel electrophoresis, and single-strand conformation polymorphism analysis. These methods can be used in conjunction with the PCR technique to detect small deletions or insertions and single base substitutions. 

III) SSCP (single stranded conformation polymorphisms) Fragments amplified from sensitive and resistant isolates of V. nashicola were analysed by capillary electrophoresis... 

The most widely used genetic screening technique, PCR-RFLP detects a mutation at a specific restriction endonuclease cleavage site at the mutation locus [9], The products from other techniques such as ARMS (amplification refractory mutation system), SSCP (single-strand conformational polymorphism), HPA (heteroduplex polymorphism), and CDCE (constant denaturant capillary electrophoresis) and PCR are usually separable using polymer networks. 

Condie, A., Ecles, R., Bprresen, A.-L., Coles, C., Cooper, C., and Prosser, J. (1993) Detection of point mutations in the p53 gene comparison of single-strand conformation polymorphism, constant denaturant gel electrophoresis, and hydroxylamine and osmium tetroxide techniques. Human Mutat. 2, 58-66. 

Highsmith WE Jr, Nataraj AJ, Jin Q, O Connor JM, Ei-Nabi SH, Kusukawa N, Garner MM, Use of DNA toolbox for the characterization of mutation scanning methods. II evaluation of single-strand conformation polymorphism analysis. Electrophoresis 1999 20 1195-203. 

Gasser RB, Monti JR, ZhuX, Chilton NB, HungG-C, Guldberg P (1997). Polymerase chain reaction-linked single-strand conformation polymorphism of ribosomal DNA to fingerprint parasites. Electrophoresis, 18 1564—1566. 

Hu M, Chilton NB, Zhu X, Gasser RB (2002). Single-strand conformation polymorphism-based analysis of mitochondrial cytochrome c oxidase subunit 1 reveals significant substructuring in hookworm populations. Electrophoresis, 23 27-34. 

Rehhein H, Mackie IM, Pryde S, et al. (1998). Comparison of different methods to produce single-strand DNA for identification of canned tuna hy single-strand conformation polymorphism analysis. Electrophoresis, 19 1381-1384. 

Endo, Y., Zhang, L., Katashima, R., Itakura, M., Doherty, E. A. S., Barron, A. E., and Baba, Y. Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by capillary and microchip electrophoresis for detection of mutations in K-ras gene. Electrophoresis, 26, 3380, 2005. 

Ren, J. and Ueland, P. M. Temperature and pH effects on single-strand conformation polymorphism analysis by capillary electrophoresis. Hum. Mutat., 13, 458, 1999. 

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