Silkworm silk

Philosamia gnthia ricini = wild-type silkworm silk. 

Films or membranes of silkworm silk have been produced by air-drying aqueous solutions prepared from the concentrated salts, followed by dialysis (11,28). The films, which are water soluble, generally contain silk in the silk I conformation with a significant content of random coil. Many different treatments have been used to modify these films to decrease their water solubiUty by converting silk I to silk II in a process found usehil for enzyme entrapment (28). Silk membranes have also been cast from fibroin solutions and characterized for permeation properties. Oxygen and water vapor transmission rates were dependent on the exposure conditions to methanol to faciUtate the conversion to silk II (29). Thin monolayer films have been formed from solubilized silkworm silk using Langmuir techniques to faciUtate stmctural characterization of the protein (30). ResolubiLized silkworm cocoon silk has been spun into fibers (31), as have recombinant silkworm silks (32). 

Silk fibers or monolayers of silk proteins have a number of potential biomedical applications. Biocompatibility tests have been carried out with scaffolds of fibers or solubilized silk proteins from the silkworm Bombyx mori (for review see Ref. [38]). Some biocompatibility problems have been reported, but this was probably due to contamination with residual sericin. More recent studies with well-defined silkworm silk fibers and films suggest that the core fibroin fibers show in vivo and in vivo biocompatibility that is comparable to other biomaterials, such as polyactic acid and collagen. Altmann et al. [39] showed that a silk-fiber matrix obtained from properly processed natural silkworm fibers is a suitable material for the attachment, expansion and differentiation of adult human progenitor bone marrow stromal cells. Also, the direct inflammatory potential of silkworm silk was studied using an in vitro system [40]. The authors claimed that their silk fibers were mostly immunologically inert in short and long term culture with murine macrophage cells. 

A spider s orb-web is formed by extrusion of a concentrated protein solution and stretching of the resulting fiber. The cross-strands, which are stronger than steel, resemble silkworm silk. The molecules contain microcrystalline p sheet domains that are rich in Gly-Ala repeats as well as polyalanine segments. The capture spiral is formed from much more elastic molecules that contain many -tum-forming sequences. These assume a springlike p spiral. See Box 2-B. 

It has been proposed, as for other silks, that the peptide chains in the stalks are aligned perpendicular to the long dimension of the fiber and are folded back on themselves many times to form a (3 sheet with only 8 residues between folds.3 The chains of silk fibroin, the major protein of silkworm silk, contain 50 repeats of the sequence 13... 

From the viewpoint of zootaxa, the silkworm and the spider belong to insect and arachnid of arthropod, respectively. Their silk proteins (fibroin for silkworm silk and spidroin for spider major ampullate silk) do not have any genetic heritage in common and their amino acids sequence compositions are different too. However, the silkworm and spider employ a similar spinning process to produce silk. Furthermore, the silkworm silk and the major ampullate silk have a number of similar structural characteristics, both at the level of the secondary protein structure and the condensed silk morphology. Therefore, for the sake of convenience, they are discussed together in some parts of this text. 

Although the amino acid sequence as well as the secondary structure of fibroin differs from those of spidroin, the fibers spun from these proteins, that is, silkworm silk and spider silk have comparable mechanical properties. These may be attributed to the structural characteristics, both at the molecular and filament level. The superior mechanical properties of silk-based materials, such as films, coatings, scaffolds, and fibers produced using reconstituted or recombinant silk proteins, are determined by their condensed structures. 

In view of the various level of structural organization, it is worthwhile to draw a comparison between natural silk fibers (silkworm silk and spider silk) and man-made silk-based materials. 

Figure 4 The defects (arrows pointed) on the natural spun silkworm silk (a), and a comparison of stress-strain curves of silks drawn at different speeds from the silkworm Bombyx mori (b).

As to fibers, it was reported that the inferior mechanical properties of silk from cocoons compared to spider silk result from the silkworm spinning process. If silkworm silk is processed at a constant pulling speed rather than constant force pulling, it possesses excellent properties, approaching the spider dragline silk (Shao and Vollrath, 2002). This suggests that the silkworm silk has the potential to produce better fibers, and the regenerated fibroin, which is easy to harvest, has the possibility to be fabricated into a reconstituted super-fiber. 

Hudson et al. used Ca(NC>3)2—MeOH to dissolve silkworm silk and did not manage to obtain good fibers directly. They also casted film from such a solution and then dissolved the film in formic acid and trifluoroacetate acid... 

Zarkoob et al. (1998, 2004) were the first to report on the electrospinning of silkworm silk and Nephila clavipes dragline protein. They used an HFIP solution of protein as the spinning dope. The resulting fibers had a wide distribution in diameter and the continuity during spinning could be significantly improved. 

Shao, Z.Z. and Vollrath, F. "Materials Surprising strength of silkworm silk." Nature 418(6899), 741 (2002). 

There are all sorts of silk that are found in nature. The stuff that is usually found in textiles comes from silkworms (Bombyx mori). They are not really worms, but the larvae of moths. They emerge from very small eggs with an incredible lust for mulberry leaves, which they consume until they are ready to. pupate and weave a cocoon around themselves. Unlike spiders, which spin silk from their rear end, silkworm silk is actually hardened saliva, which comes out of the mouth. The larva has a small spinneret on its lip, through which the silk emerges. The cocoon is formed from a single strand of silk that... 

Spider silks are a little different and much more extensible. They are made up of two proteins, spidroin I and spidroin II, which vary somewhat with species and diet. Like silkworm silk, these proteins contain high proportions of glycine and alanine (about 42% and 25%, respectively), but differ in the proportions of the other, more bulky amino acids present In addition, these proteins-have 4—9 alanine residues strung together in a block, which, in turn, are linked by glycine... 

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