Regenerated fibroin

Y.H. Wu, Q.C. Shen, and S.S. Hu, Direct electrochemistry and electrocatalysis of heme-proteins in regenerated silk fibroin film. Anal. Chim. Acta 558, 179-186 (2006). 

Rossle, M., Panine, P., Urban, V. S., and Riekel, C. (2004). Structural evolution of regenerated silk fibroin under shear Combined wide- and small-angle x-ray scattering experiments using synchrotron radiation. Biopolymers 74, 316-327. Rousseau, M. E., Lefevre, T., Beaulieu, L., Asakura, T., and Pezolet, M. (2004). Study of protein conformation and orientation in silkworm and spider silk fibres using Raman microspectroscopy. Biomacromolecules 5, 2247-2257. 

Yang, Y. H., Shao, Z. Z., Chen, X., and Zhou, P. (2004). Optical spectroscopy to investigate the structure of regenerated Bombyx mori silk fibroin in solution. Biomacromolecules 5, 773-779. 

Swinerd, V. M., Collins, A. M., Skaer, N. J. V., Gheysens, T., Mann, S., Silk inverse opals from template-directed beta-sheet transformation of regenerated silk fibroin. Soft Matter 2007, 3, 1377-1380. 

Liu H, Liu Y, Qian J, Yu T, Deng J. Feature of entrapment of glucose oxidase in regenerated silk fibroin membranes and fabrication of a L -dimethylferrocene-mediating glucose sensor. Microchemical Journal 1996, 53, 241-252. 

As to fibers, it was reported that the inferior mechanical properties of silk from cocoons compared to spider silk result from the silkworm spinning process. If silkworm silk is processed at a constant pulling speed rather than constant force pulling, it possesses excellent properties, approaching the spider dragline silk (Shao and Vollrath, 2002). This suggests that the silkworm silk has the potential to produce better fibers, and the regenerated fibroin, which is easy to harvest, has the possibility to be fabricated into a reconstituted super-fiber. 

Regenerated spidroin and fibroin dissolved in various solvents are used as spinning dope while the coagulation baths are mainly alcohol (Table 4). In addition certain fiber post-treatments, such as drawing, are used as well. 

Table 4 Examples of wet spinning processes using regenerated fibroin, spidroin, and recombinant spidroin, with different solvents and coagulation baths (Zhou et al., 2006a)...

Figure 10 A novel micron-sized hybrid of silk fibroin and CaCOj, with a hollow structure and unusual rice-like shape is synthesized in the presence of regenerated silk fibroin via aggregation-mediated crystallization (a) mineralization of HAP on the electrospun mat of silk fibroin (b).

Ha, S.W., Tonelli, A.E., and Hudson, S.M. "Structural studies of Bombyx mori silk fibroin during regeneration from solutions and wet fiber spinning". Biomacromolecules 6(3), 1722-1731 (2005). 

Huang, X.T., Shao, Z.Z., and Chen, X. "Investigation on the biodegradation behavior of Bombyx mori silk and porous regenerated fibroin scaffold". Acta Chimica Sinica 65(22), 2592-2596 (2007). 

Jeong, L., Lee, K.Y., Liu, J.W., and Park, W.H. "Time-resolved structural investigation of regenerated silk fibroin nanofibers treated with solvent vapor". Int. ]. Biol. Macromol. 38(2), 140-144 (2006). 

Marsano, E., Corsini, P., Arosio, C., Boschi, A., Mormino, M., and Freddi, G. "Wet spinning of Bombyx mori silk fibroin dissolved in N-methyl morpholine N-oxide and properties of regenerated fibres". Int. J. Biol. Macromol. 37(4), 179-188 (2005). 

Matsumoto, K., Uejima, H., Iwasaki, T., Sano, Y., and Sumino, H. "Studies on regenerated protein fibers 3. Production of regenerated silk fibroin fiber by the self-dialyzing wet spinning method". J. Appl. Polym. Sci. 60(4), 503-511 (1996). 

Motta, A., Fambri, L., and Migliaresi, C. "Regenerated silk fibroin films Thermal and dynamic mechanical analysis". Macromol. Chem. Phys. 203, (10-11), 1658-1665 (2002). 

Zhu, J.X., Shao, H.L., and Hu, X.C. "Morphology and structure of electrospun mats from regenerated silk fibroin aqueous solutions with adjusting pH". Int. ]. Biol. Macromol. 41(4), 469-474 (2007). 

Min BM et al (2006) Regenerated silk fibroin nanofibers water vapor-induced structural changes and their effects on the behavior of normal human cells. Macromol Biosci 6(4) 285-292... 

Xie F et al (2006) Effect of shearing on formation of silk fibers from regenerated Bombyx mori silk fibroin aqueous solution. Int J Biol Macromol 38(3-5) 284-288 Li C et al (2006) Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 27(16) 3115-3124... 

Madduri S, Papaloizos M, Gander B (2010) Trophically and topographically functionalized silk fibroin nerve conduits for guided peripheral nerve regeneration. Biomaterials 31 (8) 2323-2334... 

Qian J, Liu Y, Liu H et al. Characterization of regenerated silk fibroin membrane for immobilisation of peroxidase and construction of an amperometric hydrogen peroxide sensor employing phenazine methosulphate as electron shuttle. J Electroanal Chem 1995 397 157-162. 

Typical NMR spectra of the aqueous solution of the regenerated silk fibroin prepared from B. mori cocoon are shown in Fig. 3." ... 

R. Nazarov, H.J. Jin, D.L. Kaplan, Porous 3-D scaffolds from regenerated silk fibroin. Biomacromolecules 5 (2004) 718-726. 

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