Serum chick embryo

It appears that both halves of the transferrin molecule contain a recognition site for the receptor, and that both are necessary for binding. Thus, the two halves of ovotransferrin are much less effective than the whole molecule in binding to the receptor and donating iron into the chick embryo red blood cell.1143 One fragment of serum transferrin is ineffective.1124... 

There are a number of media available which are not based on a detailed investigation of growth requirements, but rather include crude mixtures of nutrients added to promote cell growth. These include lactalbumin hydrolysate (Appendix 1 Table 9) or yeast extract (Appendix 4) to provide an inexpensive source of amino acids or vitamins. Thus Melnick s monkey kidney media A and B (Melnick, 1955) contain lactalbumin hydrolysate and calf serum in Hanks and Earle s BSS, respectively. Chick embryo extract and tryptose phosphate broth (Appendix 1, Tables 11 and 12) are also used occasionally and their use is referred to where appropriate throughout the book. Mitsuhashi and Maramorosch mosquito cell medium contains lactalbumin hydrolysate, yeast extract and foetal calf serum in a specially developed saline (Mitsuhashi and Maramorosch, 1964 Singh, 1967). 

M21. Moses, A. C., Cohen, K. L., Johnsonbau, R., and Nissley, S. P., Contribution irf human somatomedin activity to the serum growth requirement of human sldn fibroblasts and chick embryo fibroblasts in culture. /. Clin. Endocrinol. Metab. 46,937-946 (1978). 

Manganese and cadmium are both carcinogens and at concentrations of 10 moll induce changes in synthetic polyribonucleotides indicative of base mis-pairing, while Mg " and show no effects. 5-Mercuriuridine triphosphate has been used as a probe of in vitro transcription studies, thiol affinity chromatography being used to separate the in vitro synthesized RNA from the cellular RNA. Zn, Cd, and Hg have been found to stimulate RNA and DNA synthesis in chick embryo cultures deprived of serum the stimulation appeared to represent a non-specific event. ... 

By the use of no-flow cytometry, Barald (1989) has isolated this subpopulation of cells from neural crest cultures and studied its behaviour under a variety of different culture conditions. The cells proliferate in the presence of 15% fetal bovine serum and high concentrations of chick embryo extract, but do not differentiate. However, in chick serum, elevated K+ or heart-, iris- or lung-conditioned medium, the cells stopped proliferating and all of the cells became neuron-like within 10 days (Barald, 1989). These cells also stained positively for choline acetyl transferase (ChAT). 

Yang, Y.W.-H., Browa D.R., Robcis, H.L., Rechler, M.M. de Pablo, F. (1993). Developmental regulation of insulin-like growth factor binding protein-2 in chick embryo serum and vitreous humor. Reg. Peptides, 48, 145-55. 

Lipases are present in the chick embryo (87) and in chicken fat (71), muscle (88), and brain (89). Kgeon heart muscle and muscles of the Idte and parakeet contain hpases (90). Lipases are present in several organs of the carp (91-93), in cyclostome and teleost fish (94, 95), in the digestive tract of goldfish and other fish (96) and of Tor tor (97), in eels (98), and in salmon serum (99). Several organs of the sea turtle and the saliva of land tortoises contain hpase (100, 101). 

Our first investigation of the possible participation of ADPRT activity in cell differentiation used a chick muscle primary cell culture system. When embryonic chick myoblasts are seeded onto collagen-coated petri dishes and grown in a medium containing horse-serum and embryo extract, the majority of the cells pass through an initial phase of proliferation (Fig. 4). The onset of terminal differentiation is marked by the fusion of myoblast cell membranes to form multi-nucleate syncytia of muscle fibres, the cessation of DNA biosynthesis and of cell division. There is also substantial increase in the activity of creatine phosphokinase, largely due to the de novo synthesis of a muscle specific isozyme of this protein. In this work we have made use of... 

Hepatocytes were prepared from livers of 14 day old chick-embryos [4]. The livers were cut into small pieces with scissors and incubated for 30 min at 37in Ca and Mg free Krebs-Hanselit buffer at pH 7.6, This was followed by a second 30 min. incubation in the presence of collagenase (0.5mg/ml) and hyaluronidase (Img/ml). At the end of the incubation period 3 volumes of M-199 medium were added and the free cells collected by centrifugation. The isolated cells were washed 3 times with M-199. They were finally suspended in M-199 in presence of 10% new-born calf serum and plated (Nunc 9 cm diameter plates 1.5 livers per plate). After 18 hr the medium and unattached cells were removed. The adherent cells were washed and incubated in fresh M-199 medium. or labelled precursors were... 

Chlorpyrifos is not mutagenic, as judged by mitotic recombination assays, and did not increase sister chromatid exchange above background in tests with chick (Gallus spp.) embryos and Chinese hamster (Cricetus spp.) ovary cells. Chlorpyrifos altered serum cortisol and decreased thyroxine concentrations in sheep given oral doses of 12.5 mg chlor-pyrifos g BW twice weekly for 43 days, indicting a need for more research on the role of chlorpyrifos in hormone metabolism. 

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