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In vitro transcription

FIGURE 13.15 Expression vectors carrying the promoter recognized by the RNA polymerase of bacteriophage SPG are useful for making RNA transcripts in vitro. SPG RNA polymerase works efficiently in vitro and recognizes its specific promoter with high specificity. [Pg.413]

Technically, in vitro transcription is achieved from standard expression plasmids typically carrying SP6 or T7 promoters using marketed kits. Translation into the polypeptide may be either coupled directly to the transcription (in vitro TnT) or require isolation of the RNA. Again, a large number of suitable prokaryotic and eukaryotic cell extracts as well as complementation factors are freely available. [Pg.590]

Gamble MJ, Erdjument-Bromage H, Tempest P, Freedman LP, Fisher R P (2005) The histone chaperone TAF-l/SET/INHAT is required for transcription in vitro of chromatin templates. Mol.Cell.Biol 25 797-807... [Pg.123]

Promega Corporation. 1991. Plasmid cloning and transcription in vitro. Promega protocols and applications guide 2nd edn, Titus DE, editor. Promega Corporation USA. [Pg.438]

Early studies established that HMGBl and HMGB2 could stimulate transcription in vitro by RNA polymerases II and III [122-124]. Subsequently these proteins, together with their counterparts in other organisms, have also been implicated in the repression of transcription. [Pg.118]

Kaiso Contains POZ domain and three Zn-fingers binds methylated DNA in a sequence specific context via Zn-fingers Inhibits transcription in vitro and in vivo partner of pi20 cathenin when localized to the cytoplasm Cytoplasmic and nuclear expressed in somatic tissues and in ES cells... [Pg.320]

This approach includes the production and purification of antisense transcripts in vitro and then the introduction of the antisense RNA into cells by microinjection. Compared to the antisense gene approach described above, a major advantage of this method is that a much larger amount of antisense RNA can be introduced into cells. Also, antisense RNA can be injected at a specific time and can therefore result in the transient inhibition of gene expression, which can be used in studies of gene expression at a specific time within a particular window of development. However, as RNAs are extremely sensitive to nuclease degradation, the potential pharmacological uses of antisense RNA are limited. [Pg.33]

Yamamoto, R., Katahira, M., Nishikawa, S., Baba, T., Taira, K. and Kumar, P.K.R. (2000) A novel RNA motif that binds efficiently and specifically to the Tat protein of HIV and inhibits the trans-activation by Tat of transcription in vitro and in vivo. Genes Cells, 5, 371-388. [Pg.108]

Termination of transcription in vitro is classified as to its dependence on the protein factor, rho (p). Rho-independent terminators have a characteristic structure, which features (a) A strong G-C rich stem and loop, (b) a sequence of 4-6 U residues in the RNA, which are transcribed from a corresponding stretch of As in the template. Rho-factor-depen-dent terminators are less well defined, as shown in Figure 10-8. [Pg.203]

Accordingly, some effort has been devoted to studying the effects of cisplatin on transcription. In vitro experiments with RNA polymerases demonstrated that productive elongation activity was prematurely terminated by the whole spectrum of cisplatin-DNA adducts, but not by the /ran.y-DDP 1,3-intrastrand adducts [150-152], Selective bypass of trans-DDP adducts was also demonstrated in XPA cells, suggesting that repair of the DNA lesions did not contribute to differential transcription inhibition by the platinum compounds [153], In vivo, hormone-induced chromatin remodeling and subsequent transcription from the MMTV promoter was specifically inhibited by cisplatin [154], In this case, platinum adducts seemed to cause a decrease in the DNA binding of one of the transcription factors, NF1. Several chromatin-associated proteins, such as the linker histone protein HI or... [Pg.93]

Moule Y, Frayssinet C, Rousseau N. 1971. Effects of acrolein on transcription in vitro. Fed Eur Biochem Soc Lett 16 216-218. [Pg.132]

It is known that the first aldehyde metabolite of alcohol, acetaldehyde, stimulates collagen transcription in vitro in hepatic fibroblasts and lipocytes [85,86], suggesting a link with alcoholic liver fibrosis in vivo. Chojkier et al. [84] have shown that the lipid-peroxidation product malondialdehyde also increases collagen production 2-3-fold in cultured foetal fibroblasts. The way in which... [Pg.371]

Figure 9.6. Cation-71 interaction in the binding of acetylcholine to the nicotinic acetylcholine receptor, a Incorporation of flu-orinated tryptophan residues into NAR a chains in vivo. Individual tryptophan codons were replaced by amber stop codons (UAA). The mRNA was obtained by transcription in vitro and injected into Xenopus laevis (frog) oocytes, along with a suppressor tRNA that had been acylated with the synthetic fluorinated trp. b Oocytes expressing the modified NAR were analyzed by patch clamp. Currents are plotted as functions of acetylcholine concentration. The EC q increases monotonously with the extent of fluorination. c Replot of the EC q as a function of a derived parameter that describes the strength of the cation-71 interaction (the derivation is beyond me). Figure 9.6. Cation-71 interaction in the binding of acetylcholine to the nicotinic acetylcholine receptor, a Incorporation of flu-orinated tryptophan residues into NAR a chains in vivo. Individual tryptophan codons were replaced by amber stop codons (UAA). The mRNA was obtained by transcription in vitro and injected into Xenopus laevis (frog) oocytes, along with a suppressor tRNA that had been acylated with the synthetic fluorinated trp. b Oocytes expressing the modified NAR were analyzed by patch clamp. Currents are plotted as functions of acetylcholine concentration. The EC q increases monotonously with the extent of fluorination. c Replot of the EC q as a function of a derived parameter that describes the strength of the cation-71 interaction (the derivation is beyond me).
Fortunately, the complicated process of the assembly of the many components of PIC at transcription initiation sites can be simplified by substituting the multicomponent TFIID complex by TBP. The TBP—TATA-box complex represents a reduced PIC which can actually carry out transcription in vitro. [Pg.164]

CaMKI is located in cytoplasm and nuclei and is likely to participate in regulation of gene transcription. In vitro CaMKI phosphorylates substrates such as synapsin and the cystic fibrosis transmembrane regulator although its in vivo substrates are yet to be identified. CaMKI is activated by phosphorylation by CaMKK when both kinases are calcium and CaM bound. The 25-residue CaM-binding peptide of CaMKI forms an o -hehcal stmcture. Two hydrophobic residues are located at positions 1 and 14 (Trp-303 and Met-316). The peptide induces the bending of the central hehx with the interaction of both the N- and C-domains of CaM (pdb 1MXE). > 1... [Pg.559]

Baneijee, A. K., and Shatkin, A. J. (1970). Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase./. Virol. 6, 1-11. [Pg.249]

C.R. Wobbe and K. Struhl. Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro. Mol. Cell. Biol. 10 (1990) 3859-67. [Pg.406]

Dilworth FJ, Fromental-Ramain C, Rem-boutsika E, Benecke A, Chambon P. 1999. Ligand-dependent activation of transcription in vitro by retinoic acid receptor alpha and retinoid X receptor alpha heterodimers that mimics transactivation by retinoids in vivo. Proc. Natl. Acad. Sci. USA 96 1995-2000... [Pg.66]

Protasova lA, Semin YA, Adler VV, et al. 1982. Influence of formaldehyde and products of its interaction with amines on processes of DNA transcription in vitro. Biochemistry 47 1509-1521. [Pg.421]


See other pages where In vitro transcription is mentioned: [Pg.166]    [Pg.350]    [Pg.75]    [Pg.248]    [Pg.259]    [Pg.120]    [Pg.131]    [Pg.1004]    [Pg.68]    [Pg.75]    [Pg.162]    [Pg.60]    [Pg.114]    [Pg.104]    [Pg.329]    [Pg.383]    [Pg.500]    [Pg.319]    [Pg.268]   
See also in sourсe #XX -- [ Pg.207 ]

See also in sourсe #XX -- [ Pg.80 , Pg.81 , Pg.87 ]




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In vitro Transcription and Translation

In vitro coupled transcription/translation

In vitro transcription and

In-vitro transcription reaction

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