Seed-specific

Shewmaker, C.K. et al.. Seed-specific overexpression of phytoene synthase increase in carotenoids and other metabolic effects. Plant J., 20, 401, 1999. 

The case of canola is extraordinary because of the very high level accumulations (50-fold) of leaf-type carotenoids in seeds when the gene was introduced under the seed-specific promoter, napin. The exalbuminous seeds of canola differ from those of genetically engineered rice cereal grains in that they have chloroplasts, which may explain the capacity for hyperaccumulation of carotenoids. 

Cereal expression systems are among the most advantageous for field-based recombinant protein production, since they combine intrinsic biosafety features (self-pollination in rice, barley and wheat, seed-specific protein expression) with practical ben-... 

T84.66 a CEA (scFv) Legumin A seed specific promo-ter/Q sequence TMV 5 UTR Light chain LP of mAb24, KDEL P. sativum (seed) Ougg-1 FW 22... 

Within each species, individual promoters resulted in distinct, tissue-dependent accumulation patterns. The cauliflower mosaic virus (CaMV) 35S promoter, for example, led to high-level accumulation in callus and leaves whereas the maize ubiqui-tin-1 promoter was the best choice for producing recombinant proteins in cereal seeds even though it is not in itself seed-specific [23]. The lack of such comparative studies for proteins other than rAbs makes it difficult to generalize an optimal expression strategy for all proteins. Tables 7.1 and 7.2 list recombinant proteins expressed in plants and provide details of the production system, promoters and other regulatory elements used in each case. 

Seed-specific production of LTB by Streatfield et al. [28] resulted in expression levels of up to 1.8% total soluble protein (TSP), and two separate maize breeding programs have increased antigen production by fivefold [28] and tenfold [27]. The investigations of Chikwamba et al. [27] regarding the expression of LTB in maize are among the first to include the use of particle bombardment transformation for the production of plant-derived vaccines. 

Based on previous experiments in tobacco and Arabidopsis, the USP promoter fragment we used was considered to be highly seed specific. However, thorough examination of the field-grown pea plants revealed some promoter activity in pollen, albeit 100-fold lower than the activity seen in seeds (Fig. 12.3). To test whether or not the pollen expression is restricted to pea, we also examined pollen from transgenic... 

Jones, E. M., and Surewicz, W. K. (2005). Fibril conformation as the basis of species- and strain-dependent seeding specificity of mammalian prion amyloids. Cell 121, 63-72. 

Jones, E. M., and Surewicz, W. K. (2005). Fibril conformation as the basis of species- and strain-dependent seeding specificity of mammalian prion amyloids. Cell 121, 63-72. Kad, N. M., Myers, S. L., Smith, D. P., Smith, D. A., Radford, S. E., and Thomson, N. H. (2003). Hierarchical assembly of beta2-microglobulin amyloid in vitro revealed by atomic force microscopy./. Mol. Biol. 330, 785-797. 

Leah, R. Mundy, J. (1989). The bifunctional a-amylase/subtilisin inhibitor of barley Nucleotide sequence and patterns of seed-specific expression. Plant Molecular Biology 12, 673-82. 

Seed-specific Fabales Lentil Lens culinaris Len c 2... 

Suh et al. (1999) studied the isoforms of acyl carrier protein involved in seed-specific fatty acid synthesis in coriander seed, ft produces unusual monoenoic fatty acids which constitute over 80% of the total fatty acids of the seed oil. The initial step in the formation of these fatty acids is the desaturation of palmitoyl-ACP (acyl carrier protein) at the DELTA4 or DELTA6 positions to produce DELTA4-hexadecenoic acid (16 1DELTA4) or DELTA6-hexadecenoic acid (16 1DELTA6), respectively. 

Mekhedov, S., Cahoon, E.B. and Ohlrogge, J. (2001) An unusual seed-specific 3-ketoacyl ACP synthase associated with the biosynthesis of petroselinic acid in coriander. Plant Molecular Biology 47(4), 507-518. 

Suh, M.C., Schultz, D.J. and Ohlrogge, J.B. (1 999) Isoforms of acyl carrier protein involved in seed specific fatty acid synthesis. Plant Journal 1 7(6), 679-688. 

OUWERKERK, P.B., MEMELINK, J., A G-box element from the Catharanthus rosues strictosidine synthase (Str) gene promoter confers seed-specific expression in transgenic tobacco plants. Mol. Gen. Genet., 1999,261, 635-643. 

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