Transgenic tobacco plants

Yan J. Wang J. Tissue D. Holaday A. S. Allen R. Zhang H. (2003) Protection of photosynthesis and seed production under water-deficit conditions in transgenic tobacco plants that over-express Arabidopsis ascorbate peroxidase // Grop Sci. V. 43. P. 1477-483. 

Cell wall properties of transgenic tobacco plants that express a yeast derived acid invertase in their vacuole... 

As discussed above, Rubisco levels have been reduced by expressing antisense RNA in transgenic tobacco plants [26]. Plants expressing antisense rbcS RNA showed reduced levels of rbcS mRNA, normal levels of rbcL mRNA, and coordinately reduced levels of LSU and SSU proteins. 

Fig. 11.3 Purification ofSOl-lOOxELP-proteins from transgenic tobacco plants by inverse transition cycling and analysis by SDS-PAGE. 1 15 pg of total soluble leaf protein extracted in raw extract buffer 2 cleared supernatant of original 15 pg total soluble leaf protein after heat treatment (60 min, 95 °C) 3 cleared supernatant of original 300 pg leaf protein after heat treatment 4 cleared supernatant of original 300 pg leaf protein after heat treatment (60 min, 60 °C) with 2 M NaCI 5 redissolved spider silk-elastin protein pellet from original 300 pg of total soluble leaf protein after heat treatment (60 min, 60 °C) with 2 M NaCI.

BRISSON, L.F., ZELITCH, I., HAVIR, E.A., Manipulation of catalase levels produces altered photosynthesis in transgenic tobacco plants, Plant Physiol., 1998,116,259-269. 

XIAO, X.W., CHU, P.W.G., FRENKEL, M.J., TABE, L.M., SHUKLA, D.D., HANNA, P.J., HIGGINS, T.J.V., MULLER, W.J., WARD, C.W., Antibody-mediated improved resistance to C1YW and PVY infections in transgenic tobacco plants expressing a single-chain variable region antibody, Mol. Breed., 2000,6,421-431. 

De Borne, F. D., Elmayan, T., de Roton, C., de Hys, L. and Tepfer, M., 1998, Cadmium partitioning in transgenic tobacco plants expressing a mammalian metaUothionein gene, Mol. Breeding 4 83-90. 

Kawashima, C., G., Noji, M., Nakamura, M., Ogra, Y., Suzuki, K. T., and Saito, K., 2004, Heavy metal tolerance of transgenic tobacco plants over-expressing cysteine synthase, Biotechnol. Lett. 26 153-157. 

Li D, O Leary J, Huang Y, Huner NPA, Jevnikar AM, Ma S. (2006) Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants. Plant Cell Rep 25 417-424. 

NTOl 2 Huang, Y., W. Liang, A. Pan, Z. Zhou, C. Huang, J. Chen, and D. Zhang. Production of FaeG, the major subunit of K88 fimbriae, in transgenic tobacco plants and its immunogenicity in mice. Infect Immun 2003 71(9) 5436-5439. 

Polashock, J.J. et al., Cloning of a cDNA encoding the cranberry dihydrofiavonol-4-reductase (DFR) and expression in transgenic tobacco. Plant Scl, 163, 241, 2002. 

Tamagnone, L. et al.. The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco. Plant Cell, 10, 135, 1998. 

Aharoni, A. et al.. The strawberry FaMYBl transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J., 28, 319, 2001. 

The coding sequences of a- and P-globins of human hemoglobin have been fused to the sequence of the chloroplastic transit peptide of the small subunit of Rubisco. These proteins were then coexpressed in transgenic tobacco plants, resulting in the production of a functional form of tetra-meric hemoglobin. The results demonstrate that a complex multimeric protein such as recombinant human hemoglobin can be obtained from tobacco in a functional form. 

Gutierrez-Ortega, A., Avila-Moreno, R, Saucedo-Arias, L.J., Sanches-Torres, C., and G6mez-Lim, M.A. (2004). Expression of a single-chain human interleukin-12 gene in transgenic tobacco plants and functional studies. Biotechnol. Bioeng. 85(7) 734-740. 

In another study, Ko et al. (2003) expressed human antirabies MAb in transgenic tobacco plants and compared its A-glycosylation patterns... 

Cabanes-Macheteau, M., Fitchette-Laine, A.-C., Loutelier-Bourhis, C., Lange, C., Vine, N.D., Ma, J.K.C., Lerouge, R, and Faye, L. (1999). V-glycosylation of a mouse IgG expressed in transgenic tobacco plants. Glycobiology 9(4) 365-372. 

Schinkel, H., Schiermeyer, A., Soeur, R Fischer, R and Schillberg, S. (2005). Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells. Transgenic Res. 14(3) 251-259. 

Prodnction of FaeG, the major snbnnit of K88 fimbriae, in transgenic tobacco plants and its immnnogenicity in mice. Infect. Immun. 71(9) 5436-5439. 

A metabolic engineering approach has now provided direct evidence for the role of salicylic acid in systemic acquired resistance. Transgenic tobacco plants were produced expressing the "nah G" gene from Pseudomonas putida, which encodes a salicylate hydroxylase that converts salicylic acid... 

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