Transformation particle bombardement

Agrobacterium-mediated stable transformation -Plant Agrobacterium-mediated stable transformation -Cell culture Transient protoplast transformation Particle bombardment-based transient expression Agrobacterium- mediated transient expression... 

The calli used to generate cell lines can be transgenic and express the vaccine or therapeutic protein of interest. Transfer of foreign genes into calli takes place by Agrobacterium-va.Q6M.td transformation, particle bombardment, electroporation of protoplasts, or by viral vectors (Fischer... 

Seed-specific production of LTB by Streatfield et al. [28] resulted in expression levels of up to 1.8% total soluble protein (TSP), and two separate maize breeding programs have increased antigen production by fivefold [28] and tenfold [27]. The investigations of Chikwamba et al. [27] regarding the expression of LTB in maize are among the first to include the use of particle bombardment transformation for the production of plant-derived vaccines. 

Finer, J.J., Finer, K.R., and Ponappa, T. (1999). Particle bombardment mediated transformation. Curr. Top. Microbiol. Immunol. 240 59-80. 

Finally, we tested the particle bombardment method, a biolistic approach that was shown before to work when other approaches have failed. In particular, particle bombardment has allowed the generation of transgenic plants such as crop species, which are not susceptible to Agrobacterium tumefaciens or cannot be regenerated from protoplasts. Furthermore, particle bombardment has facilitated organelle transformation in intact cells and the genetic modification of cultured cells that were not accessible to other transfection techniques. 

When we started to elaborate the particle bombardment technique for S. mansoni, two questions were asked. First, is it generally possible to use the biolistic approach to genetically modify schistosomes Second, if yes, does the method allow the generation of reliable and reproducible results To approach these questions, we first designed test vectors for transformation experiments. These vectors... 

Heyers, O., Walduck, A.K., Brindley, P.J., BleiB, W., Lucius, R., Dorbic, T., Wittig, B. and Kalinna, B.H. (2004) Schistosoma mansoni miracidia transformed by particle bombardment infect Biomphalaria glabrata snails and develop into transgenic sporocysts. Experimental Parasitology 1 05, 1 74-1 78. 

Klein, T.M., Arebtzen, R., Lewis, P.A. and Fitzpatrick-McElligot, S. (1992) Transformation of microbes, plants and animals by particle bombardment. Biotechnology 10, 286-291. 

D. F. Hunt, J. Shabanowitz, R. T. Mclver, Jr., R. L. Hunter, and J. E. P. Syka, "Ionization and Mass Analysis of Nonvolatile Compounds by Particle Bombardment Tandem-Quadrupole Fourier Transform Mass Spectrometry," Anal. Chem., 57, 765-768 (1985). 

A number of different methods for stable or transient genetic transformation of plants or plant cells have been developed [13-15]. These comprise particle bombardment, Agro actermm-mediated transformation, floral dip transformation, agrodrench, viral vectors, protoplast transformation and ultrasound. These are the main techniques for the genetic transformation of plants, and many of them have also been applied for the transformation of secondary metabolite pathways in an attempt to alter the metabolic pathways of target... 

Twyman, R.M. Christou, P. Plant transformation technology particle bombardment. In Handbook of Plant Biotechnology, Christou, P., Klee, H., Eds. John Wiley Sons, Ltd. Chichester, U.K., 2004 263-289. 

Plant transformation techniques allow the delivery of the transforming DNA through the cell wall and plasma and nuclear membranes, without compromising the viability of the cell. Gene delivery can be performed either via a biological vector (plant viruses or bacteria) or by non-biological vector-free procedures (chemical methods, microinjection, particle bombardment, etc.) (Birch 1997). 

Nuclear transformations change one atom into another by particle bombardment. 

FINER, J.J., MCMULLEN, M.D., Transformation of soybean via particle bombardment of embryogenic suspension culture tissue. In Vitro Cell Dev. Biol., 1991,27, 175-182. 

Particle bombardment remains the most effective method for transforming the chloroplast of C. reinhardtii, and was first demonstrated in the 1980s [64]. 

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