Section Methodology

In this section, methodological principles and reference values are given for several of the methods used to measure red cell enzymes. Methodological details of these methods are given in the Evolve site that accompanies this book at http //evolve.elsevier. com/Tietz/textbook. 

In this section, methodological variations of the various steps in SSCP analysis are discussed. 

The efficiency of the microencapsulation process for the above described formulation was not evaluated because for this new formulation other experiments on the self-healing efficiency were carried out. This analysis does not require a hard-working procedure if we use the infrared spectroscopy by way of example, hereafter, in the section "Methodologies" we report a procedure to analyze the efficiency of the microencapsulation process for microcapsules only filled with DCPD. 

This study used the PROSUITE endpoint impact assessment method in which all impacts are categorized into five main impacts, including impact on human health, impact on social being, impact on prosperity, impact on natural environment, and impact on exhaustible resources. These impacts are then combined with normalization and weighting to obtain a single sustainability score (see the section Methodologies for Assessing Life Cycle Sustainability). 

In this section, methodological aspects crucial to the art of MD will be described with a particular emphasis on structural and dynamical artifacts which appeared during the development of simulation protocols, as we believe that such discrepancies contain the. seeds, and point to directions, of future improvements. For additional aspects of nucleic acid MD... 

Where contradictory or inconsistent results are obtained by different studies, and different measures have been used, it remains a possibility that differences in the instruments or measures used are responsible for differences found. It is also true that a finding can be strengthened if it is replicated with a different measure. Similarly, as has been described in earlier sections, methodological differences may limit extrapolation from one situation to another. 

The study of quautum effects associated with nuclear motion is a distinct field of chemistry, known as quantum molecular dynamics. This section gives an overview of the methodology of the field for fiirtlier reading, consult [1, 2, 3, 4 and 5,]. 

Section VI shows the power of the modulus-phase formalism and is included in this chapter partly for methodological purposes. In this formalism, the equations of continuity and the Hamilton-Jacobi equations can be naturally derived in both the nonrelativistic and the relativistic (Dirac) theories of the electron. It is shown that in the four-component (spinor) theory of electrons, the two exha components in the spinor wave function will have only a minor effect on the topological phase, provided certain conditions are met (nearly nonrelativistic velocities and external fields that are not excessively large). 

A wider variety of reaction types involving reactions at bonds to oxygen atom bearing functional groups was investigated by the same kind of methodology [30]. Reaction classification is an essential step in knowledge extraction from reaction databases. This topic is discussed in Section 10.3.1 of this book. 

After 3 hours the stirring is stopped and the solution allowed to settle. By this time just about all the foil will have turned to dust, which is going to make the next step of vacuum filtration very difficult because it will plug up the filter paper in a second. So the chemist lets it settle, then pours off the liquid through the vacuum filtration setup (see methodology section). The flask is rinsed with lOOmL methanol, the methanol poured through the grey filter cake and the filter cake discarded. All of the filtrate is placed in a flask and vacuum distilled to remove all the methanol, isopropyl alcohol and water which will leave the chemist with oil and junk in the bottom of the flask. 

One route to o-nitrobenzyl ketones is by acylation of carbon nucleophiles by o-nitrophenylacetyl chloride. This reaction has been applied to such nucleophiles as diethyl malonatc[l], methyl acetoacetate[2], Meldrum s acid[3] and enamines[4]. The procedure given below for ethyl indole-2-acetate is a good example of this methodology. Acylation of u-nitrobenzyl anions, as illustrated by the reaction with diethyl oxalate in the classic Reissert procedure for preparing indolc-2-carboxylate esters[5], is another route to o-nitrobenzyl ketones. The o-nitrophenyl enamines generated in the first step of the Leimgruber-Batcho synthesis (see Section 2.1) are also potential substrates for C-acylation[6,7], Deformylation and reduction leads to 2-sub-stituted indoles. 

Introduction Theprevious sections dealt with techniques for the identification of hazards and methods for calculating the effects of accidental releases of hazardous materials. This section addresses the methodologies available to analyze and estimate risk, which is a function of both the consequences of an incident and its frequency. The apphcation of these methodologies in most instances is not trivial. A significant allocation of resources is necessary. Therefore, a selection process or risk prioritization process is advised before considering a risk analysis study. 

This section describes the basic methodology of normal mode analysis. Owing to its long history it has been described in detail in the context of many different fields. However, to aid in understanding subsequent sections of this chapter, it is described here in some detail. 

The CA methodology is useful in this respect. It is comprised of three sections the Component Manufacturing Variability Risks Analysis, the Component Assembly Variability Risks Analysis and the determination of the Effects of Non-conformance through the Conformability Map. 

An early version of MET methodology was applied in the Interim Reliability Evaluation Program (IREP) that analyzed the ( ill vert Cliffs and Arkansas Nuclear lessons learned in IREP and other applications. Although MET is an extension of the fault tree analysis (Section 3.4,4), it warrants a. separate discussion (see NUREG/ CR 3268). Objectives of MET are ... 

Sec. Ill is concerned with the description of models with directional associative forces, introduced by Wertheim. Singlet and pair theories for these models are presented. However, the main part of this section describes the density functional methodology and shows its application in the studies of adsorption of associating fluids on partially permeable walls. In addition, the application of the density functional method in investigations of wettability of associating fluids on solid surfaces and of capillary condensation in slit-like pores is presented. 

In subsequent chapters, the various theories, tools, and techniques required to turn the systems approach from a concept to a practical error reduction methodology will be described. The components of this methodology are described in Figure 1.7. Each of these components will now be described in turn, together with references to the appropriate sections of the book. 

Another link exists between the PIF concept and the sociotechnical assessment methods described in Section 2.7 The checklists used in the TRIPOD methodology are essentially binary questions which evaluate whether the sets of PIFs making up each of the general failure types are adequate or not. The hierarchical sets of factors in HRAM are essentially PIFs which are expressed at increasingly finer levels of definition, as required by the analyst. The audit tool which forms MANAGER also comprises items which can be regarded as PIFs which assess both management level and direct PIFs such as procedures. 

In subsequent sections the application of PIFs to various aspects of error reduction will be described. One of the most important of these applications is the use of comprehensive lists of PIFs as a means of auditing an existing plant to identify problem areas that will give rise to increased error potential. This is one aspect of the proactive approach to error reduction that forms a major theme of this book. This application of PIFs can be used by process workers as part of a participative error reduction program. This is an important feature of the human factors assessment methodology (HFAM) approach discussed in Section 2.7. 

The SPEAR framework to be described in subsequent sections is designed to be used either as a stand-alone methodology, to provide an evaluation of the human sources of risk in a plant, or in conjunction with hardware orientated analyses to provide an overall system safety assessment. The overall structure of the framework is set out in Figure 5.4. 

PROBLEM DEFINITION. This is achieved through plant visits and discussions with risk analysts. In the usual application of THERP, the scenarios of interest are defined by the hardware orientated risk analyst, who would specify critical tasks (such as performing emergency actions) in scenarios such as major fires or gas releases. Thus, the analysis is usually driven by the needs of the hardware assessment to consider specific human errors in predefined, potentially high-risk scenarios. This is in contrast to the qualitative error prediction methodology described in Section 5.5, where all interactions by the operator with critical systems are considered from the point of view of their risk potential. 

In the following sections, a number of methodologies for accident analysis will be presented. These focus primarily on the sequence and structure of an accident and the external causal factors involved. These methods provide valuable information for the interpretation process and the development of remedial measures. Because most of these techniques include a procedure for delineating the structure of an incident, and are therefore likely to be time consuming, they will usually be applied in the root cause analysis of incidents with severe consequences. 

---