Reverse-phase high-performance liquid research

A nonaqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with refractive index (RI) detection was described and used for palm olein and its fractions obtained at 12.5°C for 12-24 h by Swe et al. (101). The objective of their research was to find the optimum separation for analysis of palm olein triglycerides by NARP-HPLC, and to find a correction factor to be used in calculating CN and fatty acid composition (FAC). The NARP-HPLC method used to determine the triglyceride composition was modified from the method of Dong DiCesare (88). Palm olein was melted completely at 70°C in an oven for 30 min prior to crystal-... 

The recent popularity of HPLC as an analytical technique in biochemical and biomedical research can be attributed to the development and introduction of microparticulate reversed-phase packings in which the hydrocarbon chain (octadecyl-, ocyl-, or di-) moieties are chemically bonded to a silica base (K5). At the present time, it is estimated that approximately 80% of all HPLC separations are performed in the reversed-phase mode. Reversed-phase high-performance liquid chromatography (RPLC) has many advantages over other modes of HPLC ... 

Reversed-phase high-performance liquid chromatography is an analytical technique of tremendous importance for the separation and quantitative analysis of compounds with widely varying chemical properties. Due to the versatility, sensitivity, and reproducibility of the technique, high-performance liquid chromatographic separations have become routine in biochemical and biomedical research, especially in investigations involving nucleic acid constituents. 

Separation by reverse-phase high performance liquid chromatography and spectral characterization 35th Tobacco Chemists Research Conference, Program Booklet and Abstracts, Vol. 35, Paper No. 39, 1981, 3199. 

Reversed-phase high performance liquid chromatography (HPLC) is the preferred method of quantifying domoic acid for both regulatory and research applications. Detection can be accomplished using either UV absorbance or fluorescence, although the latter requires derivatization of the molecule but also confers greater sensitivity (Table 20.1). 

Radiolabeled [l- C]18 3n-3 was purchased from Perkin-Elmer Life Sciences (Boston, MA). The free acid form of 24 5n-3 and 24 6n-3 were generous gifts from A. Spector and H. Sprecher. Human skin fibroblasts from normal controls and patients with peroxisomal or mitochondrial disorders were received from the Mental Retardation Research Center of the Kennedy Krieger Institute. Cells at 90% confluence were incubated with 0.05 pCi albumin-bound [1- C] 18 3n-3 in Dul-becco s modified Eagle s minimum essential medium supplemented with 10% fetal bovine serum for 3 d. At harvest, cells were removed and washed with Hanks solution. An aliquot of cells was removed for protein determination, and the remainder was used for analysis of fatty acids after conversion to their methyl esters (17). Radiolabeled fatty acid methyl esters were separated by reversed-phase high performance liquid chromatography (18), and collected by a fraction collector. The radioactivity was counted by liquid scintillation counting. 

Jungalwala, EB., Hayssen, V, Pasquini, J.M., McCluer, R.H. (1979) Separation of molecular species of sphin-gomyehn by reversed-phase high-performance liquid chromatography. Journal of Lipid Research, 20, 519-587. 

As a method of research, has been used high-performance liquid chromatography in reversed - phase regime (RP HPLC). The advantage of the present method is the following the additional information about AIST and FAS composition (homologous distribution) simple preparation of samples (dilution of a CS sample of in a mobile phase). 

The rapid advancement in peptide research over the past 25 years must be attributed, in part, to the effectiveness of high-performance liquid chromatography (HPLC), particularly reversed-phase chromatography, in the separation and analysis of peptides. The resolution and selectivity of this technique allows peptides to be effectively isolated and purified from closely related substances. It also separates most or all of the components of complex biological mixtures such as tryptic digests of proteins. 

The discovery of anandamide (arachidonoyl ethanolamide, AEA) and of its manifold roles in the central nervous system and in the periphery (reviewed in refs. 1 and 2) prompted several researchers to develop analytical methods to assay and characterize the activity of the enzymes responsible for AEA metabolism in various cells and tissues. Fatty acid amide hydrolase (arachidonoyl ethanolamide amidohydrolase, EC 3.5.1.4 FAAH) has emerged as the key AEA hydrolase, showing a molecular mass of approx 64 kDa and an optimum pH of around 9.0 (3). Recently, FAAH has been crystallized,and its three dimensional structure has been determined at 2.8A resolution (4). This enzyme cleaves the amide bond and releases arachidonic acid and ethanolamine. High-performance liquid chromatography (HPLC) is the most widely used method to determine FAAH activity from different sources. We developed a new method (5) based on reversed-phase (RP)-HPLC and on-line scintillation counting, which combines the need for high resolution, reproducibility, and sensitivity... 

In 1991, a group of Freezing of Vegetable Products Department, Spanish Council for Scientific Research, Ciudad University, Madrid, Spain examined the carotenoids in four best known kiwifruit cultivars in Spain of Hayward, Abbott, Bruno and Monty by the retention times and absorption spectra of high performance liquid chromatography (HPLC) on a C-18 reversed-phase column (Table 6) (Figure 16a) (Figure 16b). 

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