Hanks’ solution

Radiolabeled [l- C]18 3n-3 was purchased from Perkin-Elmer Life Sciences (Boston, MA). The free acid form of 24 5n-3 and 24 6n-3 were generous gifts from A. Spector and H. Sprecher. Human skin fibroblasts from normal controls and patients with peroxisomal or mitochondrial disorders were received from the Mental Retardation Research Center of the Kennedy Krieger Institute. Cells at 90% confluence were incubated with 0.05 pCi albumin-bound [1- C] 18 3n-3 in Dul-becco s modified Eagle s minimum essential medium supplemented with 10% fetal bovine serum for 3 d. At harvest, cells were removed and washed with Hanks solution. An aliquot of cells was removed for protein determination, and the remainder was used for analysis of fatty acids after conversion to their methyl esters (17). Radiolabeled fatty acid methyl esters were separated by reversed-phase high performance liquid chromatography (18), and collected by a fraction collector. The radioactivity was counted by liquid scintillation counting. 

Hanks Solution. For tissue culture (sterile, phenol red added as pH (indicator) (Sigma H8389). This replaces the more expensive MEM (minimal essential medium). 

This method is based on the work of Rahn and Solari (1986). Avian ovaries are obtained as described in Section II,D, and kept in Hanks solution. The tissue... 

Electrochemical investigations were performed in Hanks solution. This is a mixture of salts and is normally used in combination with naturally occurring body substances. The ingredients of a Hanks solution are shown in Table 2.6. 

Long-term exposure tests in Hanks solution revealed that a coating of 2 p.m thickness can be successfully used for corrosion protection. However, in the presence of H2O2, which simulates an inflammatory response in the human body, a dramatic destruction of the protective coating takes place [124]. 

Peritoneal macrophages of male mice 8 h to 14 d after intraperitoneal injection of 10 mg glyceryl trioleate emulsified in Hanks solution had longer processes, more add phosphatase and more lysoso-mes than controls (Carr 1967). 

Shi P, Ng WF, Wong MH, Cheng FT. Improvement of corrosion resistance of pure magnesium in Hanks solution by microarc oxidation with sol—gel Ti02 sealing. J Alloys Compd 2009 469(l-2) 286-92. 

Potentiostatic measurements were made in Hanks solution. The solution was in a closed vessel and was not aerated or deaerated. Source A.C. Fraker etal.. Surface Preparation and Corrosion of Titanium Alloys for Surgical Implants... 

Figure 12.21 Polarization curves of stainless (d) electrodeposited PEG 400-IPTES followed steel plates in Hanks solution at a scan rate of by thermal treatment at 100 °C. (Reproduced 2 mVs (a) bare, (b) electrodeposited PEG with permission from Ref. [74].)...

Ogundele and White carried out a series of polarisation studies on surgical grade stainless steels in Hanks s physiological solution. Under... 

Ogundele, G. I. and White, W. E., Polarization Studies on Surgical-Grade Stainless Steels in Hanks Physiological Solution , in Corrosion and Degradation of Implant Materials, second symposium, (Eds) A. C. Fraker and C. D. Griffin, 117-135 ASTM Publication STP 859, Philadelphia (1985)... 

Fig. 35.—Assembled osmometer of Weissberg and Hanks." (1) Solution and reference capillary (2) solution cell (3) osmometer base (4) pressure ring (5) perforated plate (perforations not shown) (6) semipermeable membrane (7) mercury seal (8) solvent container (9) solvent level (10) cover plate ...

HBBS Hank s balanced salt solution HCA Hypertonic citrate H-CAM Hyaluronic acid cell adhesion molecule HDC Histidine decarboxylase... 

Fig. 6.10 Methods of preparation of bilayer lipid membranes. (A) A Teflon septum with a window of approximately 1mm2 area divides the solution into two compartments (a). A drop of a lipid-hexane solution is placed on the window (b). By capillary forces the lipid layer is thinned and a bilayer (black in appearance) is formed (c) (P. Mueller, D. O. Rudin, H. Ti Tien and W. D. Wescot). (B) The septum with a window is being immersed into the solution with a lipid monolayer on its surface (a). After immersion of the whole window a bilayer lipid membrane is formed (b) (M. Montal and P. Mueller). (C) A drop of lipid-hexane solution is placed at the orifice of a glass capillary (a). By slight sucking a bubble-formed BLM is shaped (b) (U. Wilmsen, C. Methfessel, W. Hanke and G. Boheim)...

Procedure A 0.002 ml aliquot of the sample solution in methanol was taken and 7 ml distilled water plus 0.1 ml Folin-Ciocalteu s phenol reagent was added and after 3 min 0.2 ml of 20% Na2C03 was included. After boiling at 90 °C (exactly 5 min) samples were cooled at room temperature and were diluted with HzO to 10 ml volume. Only distilled water and reagents were used as a blank. The absorbance of total phenolics was measured at 660 nm spectrophotometrically (a Shimadzu UV 160 spectrophotometer) as per Feldman and Hanks (1968), with a sensitivity of 0.05 pig/g d.w. A standard curve was constructed with different concentrations of ferulic acid (Serva, Germany). Concentrations of ferulic acid varied from 0.33-80jig/ml (Table 1). 

HBSS Hanks balanced salt solution (GibcoBRL, Eggenstein, Germany). 

Hank s balanced salt solution (Gibco-BRL, Grand Island, NY). Phosphate-buffered saline ([PBS] Gibco-BRL). 

Add Hank s balanced salt solution (room temperature) to the mixture to bring the vol to 50 mL, and centrifuge the cells for 10 min at 500g. 

Decant the supernatant, and wash the cells with Hank s balanced salt solution two more times. The cells should be relatively free of contaminating red blood cells and platelets after these three washing steps. 

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