Removal from media

To remove embryos before 2.5 dpf, incubate in Pronase (1-4 mg/ml in E3) for 5 min at room temperature, or until first embryos come out of chorions. Since prolonged treatment with pronase may damage embryos, immediately wash 3x in E3 ( 20 ml per wash), during which remaining embryos should come out of chorions, which should be removed from media. Pronase working solution can be reused for additional plates remove media from embryos and replace with... 

Amylase facilitates the removal of starch-containing stains such as those from pasta, potato, gravy, chocolate, and baby food. Dried-up starch is difficult to remove from medium- to low temperatures. Amylase adheres to the surface of laundry, acting as an adhesive for other stain components. Starch acts as a kind of glue which binds particulate soil to the surface. Amylase hydrolyzes the starch into dextrins and oligosaccharides the latter are readily dissolved in the washing liquor and thus successively diminish the stain. Likewise, dried-on food, in particular stains and films from starch-containing foods, may be difficult to remove in a dishwasher. 

Succinic acid was removed from medium on a packed column of sorbent. The resin XUS 40285 was tested in a packed column with simulated medium containing salts, succinic acid, acetic acid, and sugar. The packed column completely separated the fermentationbyproduct, acetate, from succinate. A simple hot water regeneration successfully concentrated succinate from 10 g/L (inlet) to 40-110 g/L in the effluent with a pH of about 5.0. The end pH indicates that succinate salt was sorbed from the "medium" and released in a partially acidic form by the hot water regeneration. If successful, this would lower separation costs by reducing the need for chemicals for the initial purification step. 

During the production of mineral oils from vacuum distillates, one of the process steps, dewaxing , removes the high melting point materials in order to improve the oil s pour point. Dewaixing produces paraffins and waxes, the first coming from light distillates, and the second from medium or heavy distillates. 

M ass Process. In the mass (or bulk) (83) ABS process the polymerization is conducted in a monomer medium rather than in water. This process usually consists of a series of two or more continuous reactors. The mbber used in this process is most commonly a solution-polymerized linear polybutadiene (or copolymer containing sytrene), although some mass processes utilize emulsion-polymerized ABS with a high mbber content for the mbber component (84). If a linear mbber is used, a solution of the mbber in the monomers is prepared for feeding to the reactor system. If emulsion ABS is used as the source of mbber, a dispersion of the ABS in the monomers is usually prepared after the water has been removed from the ABS latex. 

Hot product char carries heat into the entrained bed to obtain the high heat-transfer rates required. Feed coal must be dried and pulverized. A portion of the char recovered from the reactor product stream is cooled and discharged as product. The remainder is reheated to 650—870°C in a char heater blown with air. Gases from the reactor are cooled and scmbbed free of product tar. Hydrogen sulfide is removed from the gas, and a portion is recycled to serve as the entrainment medium. 

Traditionally, production of metallic glasses requites rapid heat removal from the material (Fig. 2) which normally involves a combination of a cooling process that has a high heat-transfer coefficient at the interface of the Hquid and quenching medium, and a thin cross section in at least one-dimension. Besides rapid cooling, a variety of techniques are available to produce metallic glasses. Processes not dependent on rapid solidification include plastic deformation (38), mechanical alloying (7,8), and diffusional transformations (10). 

Static bath mode. Feed enters at one end of the dmm and the floats exit from the other end. The sink product is removed continuously from the rotating dmm through the use of lifters attached to the dmm which empty into a launder as they move to the top. A modification of the simple dmm separator is the two-compartment dmm separator which allows a two-stage separation. In the cone-type separator (up to 6.1 m in dia and 450 t/h) feed is introduced at the top. The medium in the cone is kept in suspension by gentle agitation. The sink product is removed from the bottom of the cone either directly or by airlift in the center of the cone. The maximum particle size that can be separated is limited to 10 cm. Other separators include the Drewboy bath and the Norwaltbath (2). 

V-Trimethyl silyl diethyl amine (TMSDEA) is a stroagly basic silylatiag reageat and is particulady usehil for derivatiziag low molecular weight acids. The reaction by-product, diethylamine, is volatile enough to be easily removed from the reaction medium. 

The carbon monoxide product is removed from the top of the column and warmed against recycled high pressure product. The warm low pressure stream is compressed, and the bulk of it is recycled to the system for process use as a reboder medium and as the reflux to the carbon monoxide column the balance is removed as product. The main impurity in the stream is nitrogen from the feed gas. Carbon monoxide purities of 99.8% are commonly obtained from nitrogen-free feedstocks. 

Cocoa powder (cocoa) is prepared by pulverizing the remaining material after part of the fat (cocoa butter) is removed from chocolate Hquor. The U.S. chocolate standards define three types of cocoas based on their fat content. These are breakfast, or high fat cocoa, containing not less than 22% fat cocoa, or medium fat cocoa, containing less than 22% fat but more than 10% and low fat cocoa, containing less than 10% fat. 

Medium Boiling Esters. Esterificatioa of ethyl and propyl alcohols, ethylene glycol, and glycerol with various acids, eg, chloro- or bromoacetic, or pymvic, by the use of a third component such as bensene, toluene, hexane, cyclohexane, or carbon tetrachloride to remove the water produced is quite common. Bensene has been used as a co-solvent ia the preparatioa of methyl pymvate from pymvic acid (101). The preparatioa of ethyl lactate is described as an example of the general procedure (102). A mixture of 1 mol 80% lactic acid and 2.3 mol 95% ethyl alcohol is added to a volume of benzene equal to half that of the alcohol (ca 43 mL), and the resulting mixture is refluxed for several hours. When distilled, the overhead condensate separates iato layers. The lower layer is extracted to recover the benzene and alcohol, and the water is discarded. The upper layer is returned to the column for reflux. After all the water is removed from the reaction mixture, the excess of alcohol and benzene is removed by distillation, and the ester is fractionated to isolate the pure ester. 

Rotaiy diyers have been classified as direct, indirect-direct, indirect, and special types. The terms refer to the method of heat transfer, being direct wnen heat is added to or removed from the sohds by direct exchange between flowing gas and solids and being indirect when the heating medium is separated from physical contact with the solids by a metal wall or tube. 

The cleaning action of the pulse is so effective that the dust layer may be completely removed From the surface of the fabric. Consequently, the fabric itself must sei ve as the principal filter medium for at least a substantial part of the filtration cycle. Woven fabrics are unsuitable for such service, and felts of various types must be used. The bulk of the dust is still removed in a surface layer, but the felt ensures that an adequate collection efficiency is maintained until the dust layer has formed. 

In practice, cake filtration is used more often than filter-medium filtration. Upon achieving a certain thickness, the cake must be removed from the medium. This can be accomplished by the use of various mechanical devices or by reversing the flow of filtrate back through the medium (hence, the name baclflushing). 

Drying The process of fluid removal from a medium, either by heat or vacuum. 

Briefly stated, the production of chloramphenicol by the surface culture method involves inoculating a shallow layer, usually less than about 2 cm, of a sterile, aqueous nutrient medium with Streptomyces ver)ezuelae and incubating the mixture under aerobic conditions at a temperature between about 20° and 40°C, preferably at room temperature (about 25°C), for a period of about 10 to 15 days. The mycelium is then removed from the liquid and the culture liquid is then treated by methods described for Isolating therefrom tne desired chloramphenicol. 

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