Rat fibroblasts

Alexandrova, A.Y., Dugina, V.B., Paterson, H., Bershadsky, A.D., Vasiliev, J.M. (1993). Motility of intracellular particles in rat fibroblasts is greatly enhanced by photbol ester and by overexpression of normal p2l. Cell Mot. Cytoskel. 25, 254-266. 

The central unit of these peptidomimetics imitates a /1-turn and brings the NH2-terminus of the cysteine analogue and the CO OH terminus of the methionine in spatial proximity these can then complex the Zn2+ ion which is essential for activity of the FTase [26]. The free acid 7 inhibits the enzyme with an IC50 value of 1 nmol/1, whilst in intact cells the methyl ester 8, despite its weaker in vitro activity, is significantly more potent because it can penetrate the plasma membrane better due to its lower polarity. This property can be used to convert the morphology of H-Ras-transformed cells back to the normal form and to inhibit growth of these cells, whereas the substance shows no effect on Src-transformed and untransformed rat fibroblasts. The inhibitor therefore acts selectively on transformed cells and does not influence growth of normal cells. This result is noteworthy because farnesylation of the wild type H-Ras protein... 

Figure 9. Aflatoxin-induced UDS in primary rat fibroblasts (redrawn from Ref. 12)...

Cacace AM, Guadagno SN, Krauss RS, Fabbro D, Weinstein IB (1993) The epsilon isoform of protein kinase C is an oncogene when overexpressed in rat fibroblasts. Oncogene 8 2095-2104... 

Carnosine can affect gene expression. Ikeda et al. (1999) showed that carnosine markedly upregulates vimentin synthesis in cultured rat fibroblasts, while an association between carnosine and vimentin, a cytoskele-tal, intermediate filament protein has been noted in glial cells and neurons (Bonfanti et al., 1999). Interestingly, it has also been shown that the protease, oxidized protein hydrolase (OPH), is coexpressed with vimentin in COS cells (Shimizu et al., 2004). Thus, it is at least possible that carnosine could induce synthesis of OPH in the cultured human fibroblasts and thereby increase the cellular ability to eliminate oxidized... 

Ikeda, D., Wada, C., Yoneda, C., Abe, H., and Watabe, S. (1999). Carnosine stimulates vimentin expression in cultured rat fibroblasts. Cell Struct. Fund. 24, 79-87. 

Cytotoxic activity. Ethanol (90%) extract of the dried entire plant, in cell culture at a concentration of 0.25 mg/mL, was active on human lymphocytes Veto cells, effective dose (EDljo 0.36 mg/mL Chinese hamster ovary cells, EDjo 0.44 mg/mL and Dalton s lyphoma, EBj LZ mg/mL . Methylene chloride extract of the dried leaf, in cell culture, produced weak activity on CA-colon-SW 480, inhibitory concentration (IO506.I p,g/mL. A concentration of 500 ppm was inactive on CA-human-colon-CO-115 . Methylene chloride extract of the dried root, in cell culture at a concentration of 500 ppm, was inactive on CA-human-co-lon-CO-115 and active on CA-colon-SW 480, IC50 3.6 p-g/mL. Methanol extract of the dried root, in cell culture at a concentration of 500 ppm, was inactive on CA-colon-SW 480 and CA-human-colon-CO-115 . Ethanol (50%) extract of the seed, in cell culture, was inactive on CA-9KB, ED50 greater than 20 pg/mL 5 Water extract of the dried seed, in cell culture at a concentration of 500.0 pg/mL, produced weak activity on CA-mammary-microal-veolar . Water extract of the dried seed, in cell culture at a concentration of 500 pg/ mL, was inactive on CA-JTC-26 . Seed oil, in cell culture at concentrations of 0.01% and 0.1%, was inactive on the rat fibroblasts. A concentration of 1% produced weak activity " " . Seed oil, in cell culture at... 

GVA, Gene mutation, Fiseher 344 rat fibroblasts, hprt loeus in vivo - 100 ip X 1 Khan Heddle (1991)... 

Feeder cells for fusion cultures (see Note 6) essential for fusions employing rat myelomas. Quickly thaw irradiated rat fibroblasts, prepared as described in Note 6, just before commencing the cell fusion. Add the cells to 10 mL of serum-free DMEM, centrifuge, and wash once in serum-free DMEM Resuspend feeders in HAT medium just before addition of the fusion mixture. Alternatively, use thymocytes from spleen donors (mouse). 

Prepare a suspension of mouse thymocytes or irradiated rat fibroblasts as feeders (5 x 105/20 mL of HT or DMEM containing 10% FCS)... 

Cyclic RGDfK PEG Mouse osteoblasts and melanocytes, rat fibroblasts Electron beam patterning of nanopattemed Au-nanodots on PEGmodified substrates, backfill with peptide (with lysine anchor group) 2004 [149]... 

Tomida, M., Koyama, H., and Ono, T., A serum factor capable of stimulating hyaluronic acid synthesis in cultured rat fibroblasts, J. Cell Physiol., 91, 323, 1976. 

Innerarity, T. L., Pitas, R. E., and Mahley, R. W., Disparities in the interaction of rat and human lipoproteins with cultured rat fibroblasts and smooth muscle cells. Requirements for homology for receptor binding activity. J. Biol. Chem. 255, 11163-11172... 

The first evidence for this modification in mammalian cells was obtained when human Ha-Ras was transfected into rat fibroblasts and was found to be methylated at a C-terminal prenylated cysteine [38]. Based on the previous studies in yeast and jelly fungi, it was suggested this occurred by a third class of methyltransferase. Although Ras proteins have httle structural and sequence similarity to the fungi and yeast peptide substrates, they all have a unique characteristic a prenylated cysteine residue at the carboxyl terminus. The authors thus proposed that this motif is responsible for the recognition of this new class of methyltransf erase. 

Rat fibroblast cells Gene mutation lad) No data + Lezon-Geyda et al. 1996 CH... 

Feeder cells for fusion cultures ( eNote 5. Essential for fusions employing rat myelomas) Three hours to one day before fusion, 2—4 x 10 rat fibroblasts in 10 mL of DMEM and contained in a 30 mL plastic universal are irradiated with about 30 Gy (3000 rad) of X- or T rays. Dilute with 90 mL of HT (3 h before fusion) or DMEM containing 10% FCS (1 d before fusion) and plate 1-mL aliquots into four 24-well plates or 0.2-mL aliquots into five 96-welI plates. Alternatively, use thymocytes from spleen donors (mouse). 

Prepare a suspension of mouse thymocytes (2.5 x 10 /mL of DMEM-10% FCS) and use direcdy or seed irradiated rat fibroblasts in DMEM-10% FCS at 5 X 10 cells per well into 96-well plates for use the next day. 

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