Quantitation gas chromatography

G. Guichon and C.L. Guillemin, Quantitative Gas Chromatography for Laboratory and On-Line Process Control, Elsevier, Amsterdam, 1988. ISBN 0444428577. 

It should be noted here that the difficulty of accurately injecting small quantities of liquids imposes a significant limitation on quantitative gas chromatography. For this reason, it is essential in quantitative GLC to use a procedure, such as the use of an internal standard, which allows for any variation in size of the sample and the effectiveness with which it is applied to the column (see Sections 9.4(5) and 9.7). 

Grob, K., Split and Splitless Injection for Quantitative Gas Chromatography, Wiley-VCH, Weinheim, 2001. 

Consider the quantitative gas chromatography analysis of alcohol-blended gasoline for ethyl alcohol by the internal standard method, using isopropyl alcohol as the internal standard. The peaks for these two substances are well resolved from each other and from other components. Assume there... 

Since the mass chromatograph provides molecular weight data, the instrument is ideally suited for quantitative gas chromatography. Considerable time and uncertainty are saved using the system compared with flame ionization and thermal conductivity detectors which require response factors for each and every compound. 

Campo, E., Ferreira, V., Escudero, A., Marques, J. C., and Cacho, J. (2006). Quantitative gas chromatography-olfactometry and chemical quantitative study of the aroma of four Madeira wines. Anal. Chim. Acta 563,180-187. 

Samuelsson, B., Hambert, M., and Sweeley, C. C. (1970). Quantitative gas chromatography of prostaglandin E1 at the nanogram level Use of deuterated carrier and multiple-ion analyzer. Anal. Biochem. 38, 301-304. 

Extracts of muscle tissue are usually sufficiently pure for quantitative gas chromatography at this point, but fat-and-viscera samples require further cleanup. The small sample requirement of the electron capture detector permits a close check on the purification without a significant expenditure of sample. Gas chromatography provides tentative identification at this stage, even in impure samples, and guides the ensuing chromatographic cleanup steps. 

Ferreira, V., Aznar, M., Lopez, R., and Cacho, J. (2001a). Quantitative gas chromatography-olfactometry carried out at different dilutions of an extract. Key differences in the odor profiles of four high-quality Spanish aged red wines. J. Agric. Food Chem., 49, 4818-4824. 

Spencer GF, Plattner RD, Powell RG. Quantitative gas chromatography and gas chromatography-mass spectrometry of Cephalotaxus alkaloids. J. Chromatogr. 1976 120 335-341. Grem JL, Cheson BD, King SA, Leyland-Jones B, Suffness M. Cephalotaxine esters antileukemic advance or therapeutic failure J. Natl. Cancer Inst. 1988 80 1095-1103. 

By use of different detectors trace amounts of metals ranging from 10 to 10 g can be determined (Tables l.l/II, 1.2). Polyfluorinated 3-diketones as ligands give more highly volatile derivatives and above all allow the use of the electron capture detector (ECD) which has low detection limits for fluorinated compounds. Nevertheless decompositions can also appear in this class of substances. In addition thioketones and amino derivatives are used as chelating agents for quantitative gas-chromatography. 

Grob K. Split and splitless injection for quantitative gas chromatography Concepts, processes, practical guidelines, sources of error, 4 ed. Weinheim, Germany ... 

Gough and Baker studied a number of conventional and modified stationary phases in order to find the best one for quantitative gas chromatography of heroin and structurally related compounds. Silanized 0V-210 was found to be the most suitable for the separation of heroin, codeine, acetylcodeine, morphine and 6-0-monoacetylmorphine. It gave the best reproducibility of retention times and less losses of the compounds by adsorption. For quantitative analysis 2.8 m by 4 mm I.D. glass columns and Diatomite CLQ, 80-100 mesh, as solid support were used at a column temperature of 225°C. Despite the fact that some of the compounds, particularly morphine, suffered adsorption losses during gas chromatography, these losses were reproducible, and satisfactory quantitative data could be obtained, as shown in Table 14.19. 

G. Guiochon and C. L. GuiUemin, Quantitative Gas Chromatography For Laboratory Analysis and On-Hne Process Control, Elsevier, New York, 1988. 

K7. Kittinger, G. W., Quantitative gas chromatography of adrenal cortical steroids. Steroids 11, 47-71 (1968). 

L.A. Kogan, Kolichestvennaya Gazovaya Khromatografia (Quantitative Gas Chromatography), Khimiya, Moscow, 1975. 

Rullkotter, j., Mackenzie, A. S., Welte, D. H., Leythaeuser, D. Radke, M. 1984. Quantitative gas chromatography-mass spectrometry analysis of geological samples. Organic Geochemistry, 6, 817-827. 

Wilson, R., Smith, R., Wilson, R, Shephard, M.J., and Riemersma, R.A. 1997. Quantitative gas chromatography-mass spectrometry isomer-specific measurement of hydroxy fatty acids in biological samples and food as a marker of hpid peroxidation, Analytical Biochemistry, 248, 76. 

Coutts R. T, Jones G. R, and Liu, S-F (1979) Quantitative gas chromatography/mass spectrometry of trace amounts of glutamic acid in water samples. / Chromatogr Set 17, 551-554. 

Guiochon G and Guilletnin GL (1988) Quantitative Gas Chromatography. Amsterdam Elsevier. 

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