Glass columns

Samples and calibration standards are prepared for analysis using a 10-mL syringe. Add 10.00 mL of each sample and standard to separate 14-mL screw-cap vials containing 2.00 mL of pentane. Shake vigorously for 1 min to effect the separation. Wait 60 s for the phases to separate. Inject 3.0-pL aliquots of the pentane layer into a GC equipped with a 2-mm internal diameter, 2-m long glass column packed with a stationary phase of 10% squalane on a packing material of 80/100 mesh Chromosorb WAW. Operate the column at 67 °C and a flow rate of 25 mL/min. 

Many forms of chromatography have been used to separate mixtures of quinoline and isoquinoline homologues. For example, alumina saturated with cobalt chloride, reversed-phase Hquid chromatography, and capillary gas chromatography (gc) with deactivated glass columns have all been employed (38,39). 

Trihalomethanes. Wherever chlorine is used as a disinfectant in drinking-water treatment, trihalomethanes (THMs) generaUy are present in the finished water. The THMs usuaUy formed are trichloromethane (chloroform), bromodichloromethane, dibromochloromethane, and tribromomethane (bromoform). There are four main techniques for the analysis of THMs headspace, Hquid— Hquid extraction (Ue), adsorption—elution (purge—trap), and direct aqueous injection. The final step in each technique involves separation by gas—Hquid chromatography with a 2 mm ID coUed glass column containing 10 wt % squalene on chromosorb-W-AW (149—177 p.m (80—100 mesh)) with detection generaUy by electron capture. 

Benzenesulfonyl chloride [98-09-9J M 176.6, m 14.5°, b 120°/10mm, 251.2°/760mm(dec), d 1.384. Distd, then treated with 3mole % each of toluene and AICI3, and allowed to stand overnight. The free benzenesulfonyl chloride was distd off at 1mm pressure, and then carefully fractionally distd at 10mm in an all-glass column. [Jensen and Brown J Am Chem Soc 80 4042 795[Pg.121]

Winogradsky column Glass column with an anaerobic lower zone and an aerobic upper zone, which allows growth of microorganisms under conditions simitar to those found in nutrient-rich water and sediment. 

The XK column system is a medium-pressure jacketed glass column system designed for operating pressures up to 5 bar (0.5 MPa). Column dead volumes are less than 0.1% of the total column volume. Wetted materials include EPDM, TEFZEL, superpolyoxymethylene, and flurorubber. Columns use nylon nets of 10-)Lim mesh size and may be used with most SEC media with particle diameters >20 /xm. Columns are intended for use with aqueous solutions and... 

FIGURE 7.17 Separation of a complex mixture on Fractogel EMD BioSEC (S) with a column dimension of 1000 X 50 mm (Superformance glass column). The sample contained ferritin (I), immunoglobulin G (2), transferrin (3), ovalbumin (4), myoglobin (5), aprotinin (6), and vitamin B, (7). Five milliliters of the mixture was injected onto the column at a flow rate of 3 ml/min (eluent 20 mAI sodium phosphate buffer, 0.1 M NaCI, pH 7.2). 

Degassed and preswelled Bio-Gel P-6 and Sephacryl S-200 were packed in self-made glass columns (70 X 1.5 cm 140 X 1.5 cm) and equilibrated for 20 hr with H20(dest.) -t- 0.002% NaN3 to prevent microbial growth. The mass of eluted fractions was detected with a differential refractive index detector (Waters 403 RI, sensitivity 8). 

Extract the residue with ethyl ether and filter. Concentrate the ethereal extract to a residue. Dissolve the residue in benzene and chromatograph on 300 g of alumina contained In a glass column 1.5 inches in diameter to give the crude product. Elute with benzene. Crystallize this product from acetone-petroleum ether to obtain the product. 

An alternative elution technique is to transfer the powder (e.g. for bromophenol blue) to a glass column fitted with a glass-wool plug or glass sinter, and elute the dye with ethanol containing a little ammonia. The eluted solution, made up to a fixed volume in a small graduated flask, may be used for colorimetric/ spectrophotometric analysis of the recovered dye (see Chapter 17). A calibration curve must, of course, be constructed for each of the individual compounds. 

Pure nitrogen (oxygen-free), at a flow rate of 40 mL min is used as carrier gas. The dimensions of the glass column are 1.6 m length and 6 mm o.d., and it is packed with 5 per cent by weight SE-30 on Chromosorb W as the stationary phase. The column is maintained at a temperature of 165 °C. 

In 1971, Hiatt et al. found that polyethylene oxide (PEO) of molecular weight about 100000 prevented the adsorption of rabies virus to porous glass with an average pore diameter of 1250 A. The support was modified by passage of one void volume of 0.4% solution of the polymer in water, followed by 5 or more volumes of distilled water or buffered salt solution. The virus was effectively purified from the admixtures of brain tissue fluid by means of size-exclusion chromatography on the modified glass column [28]. 

Fig. 1. Preparative separation of the components of the concentrated culture fluid on the PVT-porous glass column. (/) fraction of purified rotaviruses, (2, 3) other components of the culture fluid [32]...

Gas chromatographic analysis of the product (1.5 m. by 0.5 cm. glass column, KF-54 on Chromosorb W, 60-80 mesh) showed a single peak with a retention time of 2.60 minutes at 170°. 

The fractions may be analyzed by gas chromatography for absence of solvent. For gas chromatographic analysis a 300 cm. by 0.3 cm. glass column packed with XE-60 (1.5% vjjw) coated on Chromosorb G AW DCMS (80/100 mesh) was employed. 

Slagt et al. [134] have stated that because of their thermal instability and reactivity sultones could not be easily analyzed by gas chromatography. They studied the two methods published by Martinsson and Nilsson using a Carlo Erba Fractovap G1 equipped with a flame ionization detector and a glass column (length 0.65 m OD 1/4 in.) filled with 10% OV 1 on Chromosorb W-AW (80-100 mesh). The column temperature was 230°C and the injector/de-tector temperature 275°C. The gas flow rates were N2 25 ml/min (carrier gas), H2 25 ml/min, and air 250 ml/min. One microliter of sample was injected. 

Neuraminic Acid and its Relation to Chronic Bronchitis. V. Glass Column Electrophoresis of Sputum, M. Z. Atassi, S. A. Barker, and M. Stacey, Clin. Chim. Acta, 7 (1962) 706-709. 

Activity III alumina is prepared by adding 6% (w/w) of water to neutral alumina of activity grade I. The submitters used a 50x3 cm. glass column for the chromatography. 

The diacetate was judged to be virtually pure by the submitters on the basis of a gas chromatographic analysis carried out at 150° using a glass column packed with 3% OV 17 (1 1 methyl-phenyl silicone) supported on 70-80 mesh Chromosorb W. 

All 10 PF resins were produced with Ba(OH)2 catalyst for 300 min with varying F/P molar ratio, OH/P wt %, and reaction temperatureffable 1). The resins were stored frozen at -18H until analysis. Molecular species in resol were analyzed by GO after trimethylsilylation of sample with N,0-bis(trimethylsilyl)trifluoracetamide in pyridine[2]. A glass column (3 m x 2 mm I.D.) packed with 3% OV-1 on 100-120 mesh Chromosorh W HP was applied. Injection... 

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