Purina chow diet

Male Wistar rats weighing 150-170 g were used throughout the experiment. The animals were maintained on a Purina chow diet and water ad libitum before use. Diabetes was produced by the intravenous injection of streptozotocin at a dose of 50 mg/kg body wt. 

All of the monkeys at the Oregon Primate Center receive Purina chow diet plus a daily supplement of fruit. Monkeys at other geographical locations within the United States receive either Purina chow or Wayne chow with fruit supplementation. The Purina chow diet contains 25% protein, 5 to 6% lipid, and approximately 55% carbohydrate, mainly in the form of starch. Analyses of the lipids show that triglyceride constitutes about 2.5% and cholesterol <0.01% of the total chow diet. 

Male Sprague Dawley rats, seven days post weanling (Bantin and Kingman, Inc., Fremont, CA) were fed pelleted rodent chow (5001, Ralston Purina Co., St. Louis, MO) or modified AIN-76 diets (BioServ, Inc., Frenchtown, NJ) with or without added sulfur amino acids. Chow, diets, and water were supplied to the rats ad libitum. The AIN-76 diet (16) was modified to contain 12% casein (normally 20% casein), and the weight difference made up with cornstarch. The normal methionine supplement was not included. The cornstarch and sucrose portions were held separately from the casein/vitamin/mineral (CVM) mixture for the purpose of mixing amino acids with the cornstarch and sucrose (CsS). Methionine (MET), methionine sulfoxide (MSO) and cysteine sulfinic acid (CSA) were obtained from Sigma Chemical Co., St. Louis, MO. Cysteine monoxide (CMO) was prepared by the method of Savige et al., (17). 

Ralston Purina complete guinea pig chow diet No. 5025. 

Baseline, chow diet (Purina 5001) Control, chow diet plus 0.25% cholesterol (w/w) and 5% soybean oil "Lovastatin," Control diet plus Lovastatin (50 mg/kg) "Unchole," Control diet plus "Unchole" (25 g/kg HDL-C was determined by the following formula (HDL-C) = TC — (LDL-C) — (VLDL-C). 

Everted intestinal sacs were prepared following the method developed by Wilson and Wiseman (25). Rats (mean weight of 210g, Slmonsen Laboratories, Gilroy, C ) which were fed a chow diet (Ralston Purina, Inc., St. Louis, MO), were killed by a blow to the head after a 13 hour fast. The small intestine was sectioned at a distance of 10% of the total length of the intestine from pylorus to the ileo-caecal junction. Any residual, undigested food was washed out with chilled 0.9% NaQ before the intestine... 

Microsomal desaturation of fatty acids. Two-month-old female Wistar rats fed on Purina chow ad libitum were used. When specified, some rats were fasted for 48 hr and then refed on a diet containing 35% protein (Peluffo and Brenner, 1974). Liver microsomes were separated by differential centrifugation at 100,000 xg in the cold in the way described by Castuma et al, 1972. 

The nematode, Nematospiroides dubius, was selected for the assay. Infected mice were fed for six days with milled Purina Lab Chow which had been mixed with the fermentation product to be tested. The mice were then fed a normal diet and, at 14 days postinfection, fecal pellets were examined for the presence of eggs. If eggs were absent on three successive days, the mice were sacrificed and their small intestines examined for the presence of worms. 

In these experiments adult, male Fischer-344 rats were fed a purified diet, AIN-76A, containing 5 or 10% citrus pectin replacing cornstarch or one of two cereal based diets, Purina Rodent Chow 5002 and NIH-07. After 28 days of dietary treatment rats were given a single oral dose of tritiated DNT (10 or 75 mgAg). Twelve hours after dosing, animals were killed and CVB was determined by exhaustive extraction. The cecum was also excised from these animals and microflora characterized by anaerobic culture techniques (10). 

Female white rats obtained from Holtzman (Madison, Wise.) were used. The animals were held in our animal quarters for 2 weeks after their arrival. They were maintained on a diet of Purina Micro-mixed Laboratory Chow, fed ad libitum, and weighed approximately 190 grams each at the beginning of an experiment. 

Control groups were fed commercial chow (Purina) or a complete diet prepared of purified components (8). The mineral mixture in this latter diet could be prepared without iron, copper or zinc so that these mineral elements could be added in controlled amounts as supplements in the form of ferrous sulfate, copper sulfate, or zinc carbonate. Under ether anesthesia, blood was removed from the abdominal aorta with a 1.5 inch, 22 gauge needle attached to a 10 ml syringe containing dried heparin. Aliquots were taken for hemoglobin, hematocrit, red cell counts and plasma analysis. The liver was removed, weighed, frozen and saved for analysis. 

We collected the odors of MHC-congenic rats ( PVG PVG.Rl) maintained on a standard lab chow (Purina) or on a test diet designed to prevent the growth of caecal bacteria (Teklad TD 69466). Rats tested in the habituation-dishabituation task could discriminate between the urinary odors of genetically identical rats after the change from Purina to... 

Diet groups based on defined diets from Ralston Purina. Each group value is the mean SEM for six rats. The diets are compared to the chow group using the Student s t test. NSD is not statistically significant (p > 0.05). The data and experimental detail are from Ref. 28. 

Table 1 gives the data for the NAD-NADH and NADP-NADPH analyses. From Table 1 it is apparent that the treatment with special diets and DMN had a profound effect on the hepatic concentrations of these coenzymes. The values for the L, N, and H groups were 45-65 % lower than the corresponding values for the control group (animals fed Purina Rat Chow). In the kidney the results were less dramatic. The only numbers that differed significantly (p < 0.02) from the results for the control group were the NAD-NADH values for the L group. 

Female Senear mice (3-4 weeks old) were purchased from Charles River Breeding Laboratories (Kingston, NY). The animals were kept in our animal facility for at least one week before use. The mice were given Purina Laboratory Chow 5001 diet and water ad libitum and they were kept at a 12 h light/12 h dark cycle during this adaptation period. 

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