Pseudomonas putida, and

L-Amino adds could be produced from D,L-aminonitriles with 50% conversion using Pseudomonas putida and Brembacterium sp respectively, the remainder being the corresponding D-amino add amide. However, this does not prove the presence of a stereoselective nitrilase. It is more likely that the nitrile hydratase converts the D,L-nitrile into the D,L-amino add amide, where upon a L-spedfic amidase converts the amide further into 50% L-amino add and 50% D-amino add amide. In this respect the method has no real advantage over the process of using a stereospecific L-aminopeptidase (vide supra). 

Harayama S, M Rekik, M Wubbolts, K Rose, RA Leppik, KN Timmis (1989) Characterization of five genes in the upper-pathway operon of TOE plasmid pPWWO from Pseudomonas putida and identification of the gene products. J Bacterial 171 5048-5055. 

Ackerley DF, CF Gonzalez, CH Park, R Blake, M Keyhan, A Matin (2004) Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli. Appl Environ Microbiol 70 873-882. 

Immobilization of neutral xenobiotics in soils by qnatemary ammoninm cations has been established, and its significance on the bioavailability of naphthalene to bacteria has been examined. Bioavailability was determined by the rates of desorption, and these differed between a strain of Pseudomonas putida and one of Alcaligenes sp. (Crocker et al. 1995). 

The degradation of toluene has been studied extensively in strains of Pseudomonas putida, and details of the three different pathways have been resolved. 

Ahmad D, M Sylvestre, M Sondossi (1991) Subcloning of bph genes from Pseudomonas testosteroni B-356 in Pseudomonas putida and Escherichia colt evidence for dehalogenation during initial attack on chloro-biphenyls. Appl Environ Microbiol 57 2880-2887. 

Guerin WF, SA Boyd (1992) Maintenance and induction of naphthalene degradation activity in Pseudomonas putida and an Alcaligenes sp under different culture conditions. Appl Environ Microbiol 61 4061-4068. 

The novel mechanism of transcriptional control of the ferric citrate transport system via transmembrane signalling is also observed in Pseudomonas putida and probably also occurs in Pseudomonas aeruginosa. Synthesis of the PupB outer... 

Thus a number of enzymes have been shown to be able to control the oxidation of sulfides to optically active sulfoxides most extensive investigations have concentrated on mono-oxygenases (e.g. from Acinetobacter sp., Pseudomonas putida) and haloperoxidases1 071 (from Caldariomyces fumago and Coral I ina officinalis). A comparison of the methodologies11081 led to the conclusion that the haloperoxidase method was more convenient since the catalysts are more readily available (from enzyme suppliers), the oxidant (H2O2) is cheap and no cofactor recycling is necessary with the haloperoxidases. Typical examples of haloperoxidase-catalysed reactions are described in Scheme 24. 

Khan, A. A. Walia, S. K. (1990). Identification and localization of 3-phenylcatechol dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase genes of Pseudomonas putida and expression in Escherichia coli. Applied and Environmental Microbiology, 56, 956-62. 

Eggink, G., Lageveen, R., Altenburg, B. Witholt, B. (1987a). Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli. Journal of Biological Chemistry, 262, 17 712-18. 

Methionine 7-lyase, in contrast, has been purified from Aeromonas sp., Pseudomonas putida and Clostridium sporogenes (361. This enzyme effects a a,y-elimination of methanethiol and ammonia from L-methionine with the direct formation of 2-ketobutyrate ... 

LC50) > 100 mg/L [Leuciscus idus], (LC50 96hr) > 50 mg/L [Fish], (EC50 3hr) >10 g/L [Bacteria], No effects observed in tests on Pseudomonas putida and e-coli. 

Metabolism of catechol (a) by catechol 2,3-dioxygenase in Pseudomonas putida and (b) by catechol 1,2-dioxygenase in Moraxella sp. 

Diniz, C.S., Voss, I., and Steinbuchel, A. (2006) Optimization of cyanophycin production in recombinant strains of Pseudomonas putida and Ralstonia eutropha employing elementary mode analysis and statistical experimental design. Biotechnol. Bioeng., 93,... 

The structure of the substrates is not necessarily restricted to monocyclic aromatic compounds such as those shown in Scheme 16.1-2. The dioxygenase activity of Pseudomonas putida and Beijerinckia species has been used exclusively for the synthesis of cis dihydrodiols from polycyclic 202 and heterocyclic 203 derivatives. Such products have been obtained from naphthalene, anthracene, phenanthrene, benz[a]pyrene, benz[a]anthracene, and methylsubstituted benz[a]anthracenes, and... 

The inhibitory effects of CTAB are reported in the literature with a high diversify of results depending on the concentration. CTAB presented inhibitory effects in concentrations above 0.01% mlv in a study with Pseudomonas putida and P. fluorescens [11, 12]. On the other hand, Fengjiao et al. [13] showed that the inhibitory effect on the growth of P. putida was pronounced in concentrations above 0.001% mlv. In this work, the concentration of 0.001% demonstrated an inhibitory effect in samples B and C. 

Koga, H., B. Rauchfrtss, and l.C. Gunsalus (1985). P450cam gene cloning and expression in Pseudomonas putida and Escherichia coli. Biochem. Biophys. Res. Commun. 130, 412-417. 

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