Proteins in potatoes

Rosahl S, Schmidt R, Schell J, Willmitzer L (1986) Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein in potato tubers. Mol Gen Genet 203 214-220... 

Developmental promoters that have been commonly used for vaccine production include patatin and E8 promoters derived from potato and tomato, respectively. Patatin is one of the major soluble proteins in potato tubers and is encoded by a multigene family. The patatin promoter is tuber-specific the protein is expressed in tubers but not in leaves (Grierson et al., 1994). Patatin has been shown to express vaccine protein in potato tubers at levels greater than the CaMV 35S promoter (Rocha-Sosa et al., 1989). 

Ryan, C. A., Narvaez, J., Pearce, G. L., MeGurl, B. F. (2005). Methods for increasing the amount of protein in potato tubers. United States Patent Applieation 20050223441. 

Smeda, R.J., PM. Hasegawa, P.B. Goldsbrough, N.K. Singh, and S.C. Weller (1993). A serine-to-threonine substitution in the triazine herbicide-binding protein in potato cells results in atrazine resistance without impairing productivity. Plant Physiol., 103 911-917. 

The patatin family consists of various glycoproteins in plants making up more than 40% of the total soluble protein in potato tubers. Patatins serve as storage proteins and it has been demonstrated that they exhibit both lipid acyl hydrolase and acetyl transferase activities, which might be involved in tissue wounding responses. Further, recent studies report on antioxidant activities of the major potato allergen Sola t 1 (Seppala et al. 2000). 

Attempts to purify the potato lectin on ovomucoid- and N,N -diacetylchitobiose-substituted Sepharose failed the lectin was so strongly bound that it could not be removed from the adsorbent by displacement with urea.483 To circumvent this difficulty, Delmotte and coworkers483 employed p-aminobenzyl 2-acetamido-2-deoxy-l-thio-/3-D-glucoside-substituted Sepharose, and eluted the lectin with 0.1 M acetic acid (yield 58 mg from 128 g of the protein in potato-tuber extract). 

One of the most successful preparations to date is that of an edible vaccine for hepatitis B.66 Volunteers who had been previously immunized parenterally (by injection, not by mouth) with the licensed, recombinant hepatitis B vaccine in yeast were given three doses over a period of 28 days of the hepatitis B antigen (HBsAg) as a recombinant protein in potatoes. The doses consisted of 100 to 110 grams of the potato that were ingested by the volunteers. Nine of the 17 volunteers responded with significant antibody production over those values measured before they ingested the potatoes. The serum... 

Several examples of proteins involved in signal transduction pathways are reported to be encoded by auxin-induced mRNAs. These include the 3-subunit of a heterotrimeric G-protein (arcA) [105,106], cyclin-dependent protein kinases (cdc2s) [107-111], and calmodulin (PCM-1 and arCAM) [112,113]. Putative transcription factors are also represented in the list of auxin-induced mRNAs. Auxin-responsive cDNA clones for a G-box binding bZIP transcription factor (SGBF-1) [114] and a homeobox transcription factor (Athb-8) have been reported [115]. Another auxin-responsive mRNA, dbp, was proposed to be a lysine-rich nuclear protein similar to HI histone, and the recombinant protein was shown to bind nonspecilically to DNA [116]. The amino acid sequence of dbp is, however, highly similar (i.e., 67% identity and 80% similarity) to a potato plasma membrane-associated protein called remorin [117]. The remorin protein binds to both simple and complex galacturonides as well as DNA, but is not a nuclear protein in potato... 

TAYLOR, J. L., FRITZEMEIER, K. H., HAUSER, I., KOMBRINK, E., ROHWER, F., SCHRODER, M., STRITTMATTER, G., HAHLBROCK, K., Structural analysis and activation by fungal infection of a gene encoding a pathogenesis-related protein in potato. Mol. Plant Microbe. Interact., 1990, 3,12-11. 

Ryan CA, Kuo T, Pearce G, Kunkel R. Variability in the concentration of three heat stable proteinase inhibitor proteins in potato tubers. Am Potato J 443-455, 1976. 

Production of Spider Silk Proteins in Transgenic Tobacco and Potato... 

REIMHOLZ, R., GEIGER, M., HAAKE, V., DEITING, U., KRAUSE, K.P., SONNEWALD, U., STITT, M., Sucrose phosphate synthase is regulated by metabolites and protein phosphorylation in potato tubers, in a manner analogous to the enzyme in leaves, Planta, 1994,192, 480-488. 

Because composition and nutritional properties of the major food legumes and oilseeds have been reported in numerous technical journals and books (listed above), the section devoted to composition and chemistry highlights lesser-known but potentially important sources of plant protein that have not received the same attention. Some of these food crops have been cultivated for many years so that they are not "new" sources. Such crops as winged bean, sweet potato, tropical seeds, fruits and leaves, yams and cucurbits are potential sources of protein in areas where they are grown. These are discussed in greater detail in the remaining five chapters. 

The method was initially developed as an assay of purity of Leptinotarsin, a protein in the hemolymph of the Colorado Potato Beetle (7) based on an idea of Fraenkel and Hsiao (S). Details of the method are given elsewhere ( ). Enough flies for an experiment are chilled and remain immobile in a Petri dish on ice. A volume of the solution being assayed, usually 1.5 pi is injected into the fly with a fine syringe needle. The fly is left for 10 minutes at room... 

In the body retinol can also be made from the vitamin precursor carotene. Vegetables like carrots, broccoli, spinach and sweet potatoes are rich sources of carotene. Conversion to retinol can take place in the intestine after which retinyl esters are formed by esterifying retinol to long chain fats. These are then absorbed into chylomicrons. Some of the absorbed vitamin A is transported by chylomicrons to extra-hepatic tissues but most goes to the liver where the vitamin is stored as retinyl palmitate in stellate cells. Vitamin A is released from the liver coupled to the retinol-binding protein in plasma. 

NT070 Mason, H. S., J. M. Ball, J. J. Shi, X. Jiang, M. K. Estes, and C. J. Amtzen. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci USA 1996 93(11) 5335-5340. 

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