Potato lectin

Lectin (carbohydratebinding proteins) Potato lectin Potato 50,000 50... 

Gal-P-Hyl Ara-a-Hyp GalT UDP-A Collagen repeats, X-Hyl-G Repetitive Hyp rich, KPhPhPV Collagen, core lectin Potato lectin... 

Pramod, S. N., Venkatesh, Y. P, Mahesh, P. A. (2007). Potato lectin activates basophils and mast cells of atopic subjects by its interaction with core chitobiose of cell-bound non-specific immunoglobulin E. Clin. Exp. Immunol., 748,391 01. 

The hydroxy-L-proline of the crude, mung-bean-wall preparation is not associated with the lectin259 this indicates a difference between the mung-bean-wall lectin and the lectin isolated from potato tubers. The latter is rich in hydroxy-L-proline, arabinose, and galactose260 in this respect, it shows features similar to those of the hydroxy-L-proline-rich glycoprotein ( extensin ) of the primary cell-wall, first reported by Lamport261 (see Section VII, 1). 

Ewen, S. W. B., and A. Pusztai. 1999. Effect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine. The Lancet 354 1353-1354. 

Down, R.E., Gatehouse, A.M.R., Hamilton, W.D.O., and Gatehouse, J.A., Snowdrop lectin inhibits development and decreases fecundity of the glasshouse potato aphid (Aulacorthum solani) when administered in vivo and via transgenic plants both in laboratory and glasshouse trials, J. Insect Physiol., 42, 1035-1045, 1996. 

Solanum tuberosum lectin Solanum tuberosum (potato) (GlcNAc)3... 

Although seeds are the common source of lectin activity, there are reports (some contradictory) of agglutinins occurring in leaves, stems, and roots.77,94 For example, black-locust (Robinia pseudoaccacia) lectin160 was isolated from its bark, potato (Solanum tuberosum) lectin161 from its tubers, and poke-weed (Phytolacca americanum) mitogen162 from its roots. A new class of plant cell-wall and membrane-bound lectins ( /3-lectins ) has been described.116,163,164 A treatise collected several schemes for the purification of lectins.165,1658... 

Pardoe and coworkers208 observed that treatment of a series of ten different, animal erythrocytes with pronase or neuraminidase had virtually no effect on the agglutination titer of the potato lectin. 

Attempts to purify the potato lectin on ovomucoid- and N,N -diacetylchitobiose-substituted Sepharose failed the lectin was so strongly bound that it could not be removed from the adsorbent by displacement with urea.483 To circumvent this difficulty, Delmotte and coworkers483 employed p-aminobenzyl 2-acetamido-2-deoxy-l-thio-/3-D-glucoside-substituted Sepharose, and eluted the lectin with 0.1 M acetic acid (yield 58 mg from 128 g of the protein in potato-tuber extract). 

The amino acid composition of the potato lectin is unusual in a number of respects the most abundant amino acid is hydroxy-L-proline (the first lectin reported to contain this amino acid207), 11.5% of the residues are half-cystine (compare wheat-germ agglutinin, with 20% of... 

Interestingly, Matsumoto and Osawa196 reported that p-nitrophenyl (and phenyl) 2-acetamido-2-deoxy-/3-D-glucopyranoside, at a concentration of 10 mg/ml, did not inhibit the agglutination reaction between the potato lectin and human erythrocytes, whereas both p-nitrophenyl 2-acetamido-2,6-dideoxy-/3-D-gIucopyranoside and phenyl 2-acet-amido-3-0-(D-l-carboxyethyl)-2-deoxy-j3-D-glucopyranoside, at a level of 5 mg/ml, completely inhibited the hemagglutinin reaction. The... 

In summary, the lectin from potato tubers (Solarium tuberosum) is a... 

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