1.3- Propanediol pathway engineering

Altaras, N. E. Cameron, D. C. Metabolic engineering of a 1,2-propanediol pathway in E.coli, Appl. Environ. Microbiol., 1999, 65, 1180-1185. (b) Enhanced production of... 

Metabolic engineering of a 1, 2-propanediol pathway in Escherichia coli. Appl. Environ. Microbiol, 65, 1180-1185. 

Niimi, S., Suzuki, N., Inui, M., and Yukawa, H. (2011) Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum. 

Boenigk R, Bowien S, Gottschalk G (1993) Fermentation of glycerol to PDO in continuous cultures of Citrobacter freundii. Appl Microbiol Biotechnol 38 453-457 Cameron DC, Altaras NE, Hoffman ML (1998) Metabolic engineering of propanediol pathways. Biotechnol Progr 14(116) 125... 

Cameron DC, Altaras NE, Hoffman ML, Shaw AJ. (1998). Metabolic engineering of propanediol pathways. Biotechnol Prog, 14,116-125. 

Siebert, D. and Wendisch, V.F, (2015) Metabolic pathway engineering for production of 1,2-propanediol and... 

Chotani G, Dodge T, Hsu A, Kumar M, LaDuca R, Trimbur D, Weyler W, Sanford K (2000) The commercial production of chemicals using pathway engineering. Biochim Biophys Acta 1543 434-455 Clark SW, Bennett GN, Rudolph FB (1989) Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A acetate/butyrate coenzyme A-transferase (EC 2.8.3.9) and in other solvent pathway enzymes. Appl Environ Microbiol 55 970-976 Cocks GT, Aguilar J, Lin EGG (1974) Evolution of L-1,2-propanediol catabolism in Escherichia coli by recruitment of enzymes for L-fucose and L-lactate metabolism. J Bacteriol 118 83-88... 

It is important that chemical engineers master an understanding of metabolic engineering, which uses genetically modified or selected organisms to manipulate the biochemical pathways in a cell to produce a new product, to eliminate unwanted reactions, or to increase the yield of a desired product. Mathematical models have the potential to enable major advances in metabolic control. An excellent example of industrial application of metabolic engineering is the DuPont process for the conversion of com sugar into 1,3-propanediol,... 

Cost-effective production of 1,3-propanediol, a new feedstock for polyesters, using a genetically engineered fermentation pathway (DuPont, 2003) ... 

The industrial focus on 1,3-propanediol has sparked interest in the microbial production of 1,2-propanediol. Some work has focused on the fermentation process of 1,2-propanediol as well as the metabolic engineering of pathways for its production. In early work with C. thermosaccharolyticum, various process conditions were examined such as temperature, pH, gas phase composition, and substrate concentration. This work was conducted in a volume of 2 1. The maximum cell concentration achieved was in the range of 1.0-1.3 g/1. The temperature range examined was 50-65 °C, and the optimal temperature for production was 60 °C. At higher temperatures, lactate decreased and ethanol increased. The pH range studied was from 6.0 to 7.2. At the optimal pH of 6.0, a concentration of 5.6 g/1 of 1,2-PD was obtained. Other fermentation conditions were examined such as... 

Zeng A-P (1996) Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum. Bioproc Engin 14 169-175... 

One of the first biobased products being commercialized exemplifying the promise of combining metabolic engineering with advanced process design to achieve a sustainable product with superior properties at a low cost is biobased 1,3-propanediol (PDO). Because biobased PDO is not yet produced in commercial quantities, this is a good time to take early measure of how well die biobased pathway meets the objectives of sustainable and environmentally benign production. 

Wu, Z., Wang, Z., Wang, G., Tan, T, 2013. Improved 1,3-propanediol production by engineering the 2,3-butane-diol and formic acid pathways in integrative recombinant Klebsiella pneumoniae. Journal of Biotechnology 168, 194-200. 

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