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Fermentation conditions

Due to the high sugar contents involved in the mead production, fermentation tends to be slow and requires a yeast strain as well as pH, temperature, and growth conditions that are optimal. [Pg.111]

During mead fermentation, several problems are generally encountered. For example, the anticipated alcohol content may not be achieved within the time desired. There may also be a lack of uniformity in the final product, due to differences in water content of the honey used. In some situations, such as worts with high sugar contents, successive addition of honey is needed to avoid premature termination of fermentation. This likelihood of stuck fermentation is increased as most mead is made empirically, without adjustments. This can lead to subsequent yeast refermentation and secondary fermentations by lactic and acetic acid bacteria. These can undesirably increase acidity and the production of volatile esters (Casellas, 2005). The presence of these compounds alters [Pg.111]

The must is subsequently sterilized, boiling being the most commonly used method (McConnell and Schramm, 1995 Navratil et al., 2001 Ukpabi, 2006). Heat treatments also have the potential to alter the antioxidant capacity by changing their phenolic profiles (Wintersteen et al., 2005). However, other techniques are described in the literature. These include the use of metabisulfite (sodium or potassium salts or in commercial form as Campden tablets)—releases sulfur dioxide that either kills or inactivates most microbes (McConnell and Schramm, 1995 Roldan et al., 2011), sulfur dioxide gas (Pereira et al., 2009 Ukpabi, 2006), pasteurization (McConnell and Schramm, 1995 Mendes-Ferreira et al., 2010), and ultrafiltration, with a 50-kDa molecular weight cutoff (McConnell and Schramm, 1995). Some of these methods also promote the removal of proteins by denaturation and coagulation, resulting in more rapid clarification during maturation. [Pg.112]

After sterilization, yeast is added to initiate fermentation. McConnell and Schramm (1995) recommend inoculation with no less than 10% by volume. Moreover, as the pH of honey is naturally low and because it is poorly buffered, the pH of must may drop during fermentation to a point limiting yeast efficiency. pH reduction can result from the synthesis of acetic and succinic acids by the yeast cells (Sroka and Tuszynski, 2007). While a rapid decline in pH inhibits undesirable microbial activity (Sroka and Tuszynski, 2007), it also reduces the dissociation of fatty acids in the wort, potentially slowing yeast metabolic action. For this, addition of a buffer is important to maintain the pH within a range of 3.7-4.0 throughout fermentation (McConnell and Schramm, 1995). Calcium carbonate, potassium carbonate, potassium bicarbonate, and tartaric acid are potential candidates. However, as some of these salts can add a bitter-salty [Pg.112]

Because honey is the only source of organic flavorants and sugar, its composition clearly sets limits on the final quality of mead. For example, Pereira (2008) demonstrated that between a dark (heather) and light (rosemary) honey, the heather honey produced the more appreciated mead. This occurred, regardless of yeast strain or supplement. To date, yeast strains isolated from honey have not shown any advantage over easily obtained commercial strains. [Pg.113]


The complexity of the biochemical pathways, certainly for antibiotic synthesis, means that the careful and rigorous selection of the microorganism is the key to obtaining higher titers. However, most microorganisms only show thek tme potential when cultured under optimal fermentation conditions. Of... [Pg.179]

Biological—Biochemical Processes. Fermentation is a biological process in which a water slurry or solution of raw material interacts with microorganisms and is enzymatically converted to other products. Biomass can be subjected to fermentation conditions to form a variety of products. Two of the most common fermentation processes yield methane and ethanol. Biochemical processes include those that occur naturally within the biomass. [Pg.17]

Alcoholic Fermentation. Certain types of starchy biomass such as com and high sugar crops are readily converted to ethanol under anaerobic fermentation conditions ia the presence of specific yeasts Saccharomyces cerevisia and other organisms (Fig. 6). However, alcohoHc fermentation of other types of biomass, such as wood and municipal wastes that contain high concentrations of cellulose, can be performed ia high yield only after the ceUulosics are converted to sugar concentrates by acid- or enzyme-catalyzed hydrolysis ... [Pg.18]

Occurrence, Fermentation, and Biosynthesis. Although a large number of Streptomjces species have been shown to produce carbapenems, only S. cattkja (2) and S. penemfaciens (11) have been reported to give thienamycin (2). Generally the antibiotics occur as a mixture of analogues or isomers and are often co-produced with penicillin N and cephamycin C. Yields are low compared to other P-lactams produced by streptomycetes, and titres are of the order of 1—20 p-g sohdusmL despite, in many cases, a great deal of effort on the optimization of the media and fermentation conditions. The rather poor stabiUty of the compounds also contributes to a low recovery in the isolation procedures. The fermentation and isolation processes for thienamycin and the olivanic acids has been reviewed in some detail (12). [Pg.4]

Within each type of distilled spidts, wide vadations of flavor can be achieved by the type and amount of starting grains or other fermentable matedals, methods of preparation, types of yeasts, fermentation conditions, distillation process, maturation time and temperature, blending, and use of new technologies such as membrane separation. [Pg.81]

Your list could be extensive since any factor that influences the rate and/or amount of product formed, ie the fermentation conditions or the characteristics of the process organism could influence productivity, eg pH, temperature, solubility of substrate. [Pg.20]

It should be noted that, for some metabolites, the dass to which they belong depends on the fermentation conditions, for example the class to which certain antibiotics belong depends on the substrate(s) used for their production. [Pg.51]

Slight changes in the fermentation conditions can greatly affect amino add production. [Pg.245]

Measurements and control of the fermentation conditions are very important for bioprocess control as they provide knowledge and hence a better understanding of the operation. [Pg.69]

Fig. 2 Time course of lactic acid yield and its concentration in SSF with pretreatment using 0.1 mol/1 HCl at 121 C for 30 rain. Fermentation conditions temperature=37 C, pH=S.0, initial load of BCR=50 g, cellulase amount=2.5 g in 2L suspension. Fig. 2 Time course of lactic acid yield and its concentration in SSF with pretreatment using 0.1 mol/1 HCl at 121 C for 30 rain. Fermentation conditions temperature=37 C, pH=S.0, initial load of BCR=50 g, cellulase amount=2.5 g in 2L suspension.
The growth of Bacillus subtilis may take place under a variety of conditions (a) aerobic conditions, (b) using nitrate as electron acceptor, and (c) fermentative conditions with glucose provided pyruvate is available as an electron acceptor since the organism lacks pyruvate formate hydrogen lyase (Nakano and Zuber 1998). [Pg.204]

Biotransformation with flasks can be used to make gram quantities of a desired product, as shown for the 21 -hydroxylation of epothilone B [75]. In cases when greater quantities of a metabolite are needed, microbial biotransformations can be carried out in a fermentor, which will allow better monitoring and control of fermentation conditions (such as pH, oxygen and glucose levels, etc.) for reaction optimization [76]. [Pg.215]

Furthermore, via optimization of the medium composition and fermentation conditions, the maximum cell density was increased by twofold, yielding a final titer of 1.1 g L 1 of 6dEB [60]. In another approach, Murli et al. [61] evaluated the three pathways of (2,S )-methylmalonyl-CoA biosynthesis (1) Streptomyces coelicolor PCC, (2) Propionibacteria shermanii MCM/... [Pg.270]

The upstream processing element of the manufacture of a batch of biopharmaceutical product begins with the removal of a single ampoule of the working cell bank. This vial is used to inoculate a small volume of sterile media, with subsequent incubation under appropriate conditions. This describes the growth of laboratory-scale starter cultures of the producer cell line. This starter culture is, in turn, used to inoculate a production-scale starter culture that is used to inoculate the production-scale bioreactor (Figure 5.7). The media composition and fermentation conditions required to... [Pg.122]

Aldol and -Michael reactions can also proceed on the reaction products of retro-Aldol and -Michael reactions. The reverse (direct) Aldol and Michael reaction can also proceed on various intermediates. Hence, these few reactions can already form a very large variety of possible products. They, indeed, account for most of the reactivity of carbohydrates discussed below, being under pyrolysis, hydrolysis or fermentation conditions. [Pg.30]

Most of the studies05,20 271 show that a correlation between culture fluorescence and biomass concentration can be obtained mainly in the exponential growth phase. In addition, in order to obtain reproducible correlations, all of the fermentation conditions such as initial substrate concentration, pH, dissolved oxygen level, temperature, and agitation rate have to be the same. However, once the culture is past exponential growth, biomass measurement by following culture fluorescence is no longer accurate. [Pg.425]

C-terminal truncation of four amino acids had occurred. Again, different fermentation conditions (temperature, time, and serum) would cause more or less C-terminal truncation, indicating that cells expressed different levels of carboxypeptidases. [Pg.254]

The concept of impurity profiling is very important for antibiotics, since most of them are still produced by fermentation or by semisynthesis starting from fermentation products. Antibiotics are typically complex mixtures of several components and their composition depends on the fermentation conditions. Impurities due to degradation occur frequently. Commercial samples usually contain significant amounts of impurities with only minor structural differences among them, but differing widely in their pharmacological activities. These impurities can exhibit antibiotic activity, but in many cases they are inactive and sometimes even toxic. The applicability of CE in the analysis of antibiotics has been reviewed elsewhere. The use of CZE in impurity analysis of antibiotics is discussed in detail below. [Pg.261]

Much effort has been expended over the years on increasing enzyme production of T. reesei by isolation of high yielding mutants and optimizing media and fermentation conditions. Strains have been isolated that produce 2-6 times the cellulase productivity of the parent wild strain (QM 6a) in batch culture. The mutants produce higher levels of cellulase protein but the specie activity of the enzymes and the proportions of the individual components (ca. 30% endo- -glucanase, 70% o- -glucanase, and less 1% cellobiase) are similar to those of the parent. [Pg.338]


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See also in sourсe #XX -- [ Pg.84 ]

See also in sourсe #XX -- [ Pg.206 ]

See also in sourсe #XX -- [ Pg.39 , Pg.45 ]

See also in sourсe #XX -- [ Pg.256 , Pg.258 ]




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