Staining, histological

Fig. 3. (A andB) Breast carcinoma, in a core biopsy of a patient, pretreatment. Hematoxylin-eosin (H E)-stained histological sections show a poorly differentiated ductal carcinoma with evident nuclear pleomorphism and numerous mitotic figures. (A) H E, magnification x200 (B) H E, magnification x400.

VIII. Multicontrast Nonlinear Microscopy of H E Stained Histological... 

VIII. MULTICONTRAST NONLINEAR MICROSCOPY OF H E STAINED HISTOLOGICAL SECTIONS... 

Beeker 1, Beeker KF, Rohrl MH et al. Laser-assisted preparation of single eells from stained histolog-ieal slides for gene analysis. Histochem Cell Biol 1997 108 447 51. 

Becker, I., Becker, K. F., and Roller, H. 1997. Laser-assisted preparation of single cells from stained histological slides for gene analysis. Histochem.. Cell. Biol. 708 447-451. 

Ringer, D. P., Schaeeee, F. V. (1992). Polymerase chain reaction microanalysis of tumors from stained histological slides. Oncogene 7, 2355-2361. 

Histology microscopy after Giemsa or silver staining Histology microscopy after immunohistochemical staining Direct urease test a biopsy incubated in urea/indicator solution visual endpoint... 

The precancerous lesions termed aberrant crypts (AC) have been developed as an early marker for colon carcinogenesis (21). Upon treatment with colon carcinogens mice and rats develop early preneoplastic changes in the crypts of the colon that are easily observable under light microscope after methylene blue staining. Histological examination of AC foci confirmed their dysplasia nature, and are considered precursors to colon cancer (22-25). 

Toluidine blue-stained histological section (b) MIcroradlographIc Image of the same sample (Helmann, 2006). 

Figure 1.2 This is a stained histological section showing a lymph node. Beyond the boundary of the lymph node is a separate island of caneer (arrowed).

For regional profihng experiments, relatively large matrix spots can be deposited directly on the sample by the use of either a low volume automatic pipette or an automatic syringe pump attached to a small capillary (Fig. 2B). Matrix solution is typically deposited in volumes ranging from 50 nL to 1 p,L. Stained histology... 

FIGURE 8.3 Teratoma formation demonstrating differentiation into cells from each of the three germ lineages. Hematoxylin and eosin-stained histological sections of a single teratoma from injection of mESCs in vivo result in the development of structures resembling mesoderm, ectoderm, and endoderm. 

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