Polymerase chain reaction technique

Pollen analysis in combination with other techniques is still an effective tool for the authentication of the botanical origin of honey (Persano Oddo et ah, 1995 Von der Ohe et ah, 2004). It can distinguish polyfloral and different types of unifloral honeys (Mateo and Bosch-Reig, 1998). It can also indicate the percentages of different nectar contributions in honey products. A polymerase chain reaction technique and an... 

Leung, D.W., Chen, E. and Goeddel, D.V. (1989) A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique, 1, 11-15. 

Ren J, Chen Z, Juan SJ, Yong XY, Pan BR, Fan DM. Detection of circulating gastric carcinoma-associated antigen MG7-Ag in human sera using an established single determinant immuno-polymerase chain reaction technique. Cancer 2000 ... 

Torres-Alves, R. Teia dos Santos, A. (2002) Detection of histamine-produdng bacteria using polymerase chain reaction techniques and DNA probes. Fur. Food Res. Technol., 214, 178-180. 

A further significant improvement in the analysis of the RFLP took place through the use of the PCR (Polymerase Chain Reaction) technique, where a certain region of DNA is amplified, producing millions of copies... 

ASTM Guide E1873-97 Standard guide for detection of nucleic acid sequences by the polymerase chain reaction technique. Guide E2048-99 Standard guide for detection of nucleic acids of the Mycobacterium tuberculosis complex and other pathogenic mycobacteria by the.. polymerase. chain. reaction technique. www.astm.org... 

Poms RE, Anklam E, Kuhn M (2004). Polymerase chain reaction techniques for food allergen detection. J. AOAC Int, 87(6) 1391-1397. 

The ability to obtain DNA fingerprints from exceedingly small tissue samples has become possible by using the polymerase chain reaction technique (Section 11.3). 

DNA fingerprinting was developed for individualization system in 1985. Analyses using DNA profiling with variable numbers of tandem repeat polymorphism has been carried out for identification of forensic samples such as bloodstains and sectional stains. Concerning forensic hairs, several DNA analyses of hair root sheath cells have been reported. However, DNA analysis of a hair shaft has not succeed to date, because DNA recovery from a hair shaft is in order of tens of picograms and only low molecular weight DNA (below 200 base pairs (bp)) is left in the hair shape. Recently, microsatellite DNA polymorphism has been detected by the polymerase chain reaction technique. This polymorphism can be applied to DNA analysis of hair shaft. If DNA analysis is combined with microscopic and instrumental analysis in forensic examination, hair individualization can be more accurate. 

The PCR (polymerase chain reaction) technique, developed in 1983, allows scientists to amplify DNA— that is, to make billions of copies—in a very short time. With PCR, sufficient DNA for analysis can be obtained from a single hair follicle or sperm. 

Subramanian D, Albrecht S, Gonzalez JM, et al. Primary pulmonary lymphoma. Diagnosis by immunoglobulin gene rearrangement study using a novel polymerase chain reaction technique. Am Rev Respir Dis 1993 148 222-226. 

Link KH, Kornmann M, Safi F, et al (1994) Response prediction in hepatic artery infusion with Fluoropyrimidines using cell culture and polymerase chain reaction techniques. Eur J Surg Oncol 20 317-318 Link KH, Kornmann M, Leder GH, et al (1996) Regional chemotherapy directed by individual chemosensitivity testing in vitro a prospective decision aiding trial. Clin Cancer Res 2 1469-1474... 

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